Abstract
Lactobacillus plantarum P5 grew aerobically in rich media at the expense of lactate; no growth was observed in the absence of aeration. The oxygen-dependent growth was accompanied by the conversion of lactate to acetate which accumulated in the growth medium. Utilization of oxygen with lactate as substrate was observed in buffered suspensions of washed whole cells and in cell-free extracts. A pathway which accounts for the generation of adenosine triphosphate during aerobic metabolism of lactate to acetate via pyruvate and acetyl phosphate is proposed. Each of the enzyme activities involved, nicotinamide adenine dinucleotide independent lactic dehydrogenase, nicotinamide adenine dinucleotide dependent lactic dehydrogenase, pyruvate oxidase, acetate kinase and NADH oxidase were demonstrated in cell-free extracts. The production of pyruvate, acetyl phosphate and acetate was demonstrated using cell-free extracts and cofactors for the enzymes of the proposed pathway.
Similar content being viewed by others
Abbreviations
- MRS:
-
Man, Rogosa and Sharpe (1960) medium modified as in Materials and methods
- TY:
-
Tryptone Yeast Extract broth
- OUL:
-
Oxygen uptake with lactate as substrate
- DCPIP:
-
2,6-Dichlorophenolindophenol
- LDH:
-
Lactic dehydrogenase
References
Brink B, Konings WN (1982) Electrochemical proton gradient and lactate concentration gradient in Streptococcus cremoris cells grown in batch culture. J Bacteriol 152:682–686
Czok R, Lamprecht W (1974) Pyruvate, phosphoenol pyruvate and d-glycerate-2 phosphate. In: Bergmeyer HU (ed) Methods in enzymatic analysis. Verlag Chemie, Weinheim, pp 1446–1451
Götz F, Sedewitz B, Elstner EF (1980) Oxygen utilization by Lactobacillus plantarum. 1. Oxygen consuming reactions. Arch Microbiol 125:209–214
Hager LP, Lipmann F (1955) Phosphate linked pyruvic acid oxidase from Lactobacillus delbruekii. In: Colowick S, Kaplan NO (eds) Methods in enzymology, vol 1. Academic Press, New York London, pp 482–486
Hager LP, Geller DM, Lipmann F (1954) Flavoprotein catalysed pyruvate oxidation in Lactobacillus delbruekii. Fed Proc 13:734–738
Lees GJ, Jago GR (1976) Acetaldehyde: an intermediate in the formation of ethanol from glucose by lactic acid bacteria. J Dairy Res 43:63–73
Lipmann F, Tuttle KC (1945) A specific method for the determination of acyl phosphates. J Biol Chem 159:21–28
Lockridge O, Massey V, Sullivan PA (1972) Mechanism of action of the flavoprotein lactate oxidase. J Biol Chem 247:8097–8106
London J (1968) Regulation and function of lactate oxidation in Streptococcus faecium. J Bacteriol 95:1380–1387
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Man JC de, Rogosa M, Sharpe ME (1960) A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135
Murphy MG (1982) Aerobic metabolism of Lactobacillus plantarum. Dissertation, National University of Ireland
Murphy MG, Condon S (1984a) Comparison of aerobic and anaerobic growth of Lactobacillus plantarum in a glucose medium. Arch Microbiol 138:49–53
Murphy MG, Condon S (1984b) Correlation of oxygen utilization and hydrogen peroxide accumulation with oxygen induced enzymes in Lactobacillus plantarum cultures. Arch Microbiol 138:44–48
Snoswell AM (1963) Oxidised nicotinamide-adenine dinucleotide-independent lactate dehydrogenase of Lactobacillus arabinosus 17.5. Biochim Biophys Acta 77:7–19
Strittmatter CF (1959a) Electron transport to oxygen in lactobacilli. J Biol Chem 234:2789–2793
Strittmatter CF (1959b) Flavin linked oxidative enzymes in Lactobacillus casei. J Biol Chem 234:2794–2800
Sutton WB (1957) Mechanism of action and crystallisation of lactate oxidative decarboxylase from Mycobacterium phlei. J Biol Chem 226:395–405
Sullivan PA (1968) Crystallisation and properties of l-lactate oxidase from Mycobacterium smegmatis. Biochem J 110:363–371
Thornhill PJ, Cogan TM (1984) The use of gas liquid chromatography in distinguishing lactic acid bacteria. Appl Environ Microbiol 47:1250
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murphy, M.G., O'Connor, L., Walsh, D. et al. Oxygen dependent lactate utilization by Lactobacillus plantarum . Arch. Microbiol. 141, 75–79 (1985). https://doi.org/10.1007/BF00446743
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00446743