Abstract
The synthesis of the luminous system of the marine luminous bacterium Photobacterium fischeri is subject to a complex, self-regulated control system called autoinduction. The bacteria produce an autoinducer which accumulates in the medium at a constant rate (as a function of cell growth). When autoinducer reaches a critical concentration it stimulates, at the level of transcription, the synthesis of the luminous system. Autoinduction is thus viewed as an environmental sensing mechanism, which curtails the synthesis of the luminous system under dilute conditions. For several isolates of P. fischeri it was found that variations in luminescence intensity could be accounted for by correlated variations in autoinducer production.
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Abbreviations
- SSS and LM:
-
are media (see text for details)
- LU:
-
light unit
- EDTA:
-
ethylene-diaminetetracetic acid
- FMNH2 :
-
reduced flavin mononucleotide
- OD:
-
optical density
References
Beijerinck, M. W.: Die Leuchtbakterien der Nordsee im August und September. Folia microbiol., Delft. 4, 15–40 (1916)
Cline, T. W., Hastings, J. W.: Bacterial bioluminescence in vivo: Control and synthesis of aldehyde factor in temperature-conditional luminescence mutants. J. Bact. 118, 1059–1066 (1974)
Eberhard, A.: Inhibition and activation of bacterial luciferase synthesis. J. Bact. 109, 1101–1105 (1972)
Farghaly, A. H.: Factors influencing the growth and light production of luminous bacteria. J. cell. comp. Physiol. 36, 165–183 (1950)
Harvey, E. N.: Bioluminescence. New York: Academic Press 1952
Hastings, J. W.: Light to hide by: Ventral luminescence to camouflage the silhouette. Science 173, 1016–1017 (1971)
Hastings, J. W., Gibson, Q. H.: Intermediates in the bioluminescent oxidation of reduced flavin mononucleotide. J. biol. Chem. 238, 2537–2554 (1963)
Hastings, J. W., Riley, W. H., Massa, J.: The purification properties and chemiluminescent quantum yield of bacterial luciferase. J. biol. Chem. 240, 1473–1481 (1965)
Hastings, J. W., Weber, G.: Total quantum flux of isotropic sources. J. Opt. Soc. Amer. 53, 1410–1415 (1963)
Henry, J. P., Michelson, A. M.: Etudes, de bioluminescence. Regulation de la bioluminescence bacterienne. C. R. Acad. Sci. (Paris) 270, 1947–1949 (1970)
Kempner, E. S., Hanson, F. E.: Aspects of light production by Photobacterium fischeri. J. Bact. 95, 975–979 (1968)
Keynan, A., Hastings, J. W.: The isolation and characterization of dark mutants of luminescent bacteria. Biol. Bull. 121, 375 (1961)
Kiefer, L., Jannasch, H., Nealson, K.: Observations of luminescent bacteria in continuous culture. Biol. Bull. 145, 455 (1973)
Morin, J. G., Harrington, A., Nealson, K. H., Krieger, N., Baldwin, T. O., Hastings, J. W.: A light for all reasons: Versatility in the behavioral repertoire of the flashlight fish. Science 190, 74–76 (1975)
Nealson, K.: Factors controlling the appearance of spontaneous dark mutants of luminous bacteria. Biol. Bull. 143, 471–472 (1972)
Nealson, K. H., Markovitz, A.: Mutant analysis and enzyme subunit complementation in bacterial bioluminescence in Photobacterium fischeri. J. Bact. 104, 300–312 (1970)
Nealson, K. H., Platt, T., Hastings, J. W.: Cellular control of the synthesis and activity of the bacterial luminescent system. J. Bact. 104, 313–322 (1970)
Reichelt, J. L., Baumann, P.: Taxonomy of the marine, luminous bacteria. Arch. Mikrobiol. 94, 283–330 (1971)
Reichelt, J. L., Baumann, P.: Photobacterium mandapamensis Hendrie et al., a later subjective synonym of P. leiognathi Boisvert et al. J. Bact. 25, 208–209 (1975)
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Nealson, K.H. Autoinduction of bacterial luciferase. Arch. Microbiol. 112, 73–79 (1977). https://doi.org/10.1007/BF00446657
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DOI: https://doi.org/10.1007/BF00446657