Abstract
A slime-free mutant was isolated from Corynebacterium autotrophicum strain 7 C. The mutant appeared in a substrate (fructose) limited chemostat culture. No significant differences between wildtype and mutant were detected with respect to pigmentation, to growth rate, and respiratory rate at varying substrate (fructose) concentrations. During growth in chemostat culture the wildtype/mutant ratio present in the inoculum was maintained for weeks. The results do not explain the selection of the mutant in the substrate limited chemostat culture.
The slime is a polysaccharide containing glucose, mannose and galactose; the uronic acid present was not identified. Copious amounts are produced from fructose, less from lactate or during autotrophic growth. The slime is not reutilized.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baker, K.: Low cost continuous culture apparatus. Lab. Pract. 17, 817–824 (1968)
Baumgarten, J., Reh, M., Schlegel, H. G.: Taxonomic studies on some grampositive coryneform hydrogen bacteria. Arch. Microbiol. 100, 207–217 (1974)
Blackkolb, F., Schlegel, H. G.: Regulation der Glucose-6-phosphat-Dehydrogenase aus Hydrogenomonas H 16 durch ATP und NADH2. Arch. Mikrobiol. 63, 177–196 (1968)
Gawehn, K.: d-Mannose und d-Mannose-6-phosphat. In: Methoden der enzymatischen Analyse, H. U. Bergmeyer, Bd. II, pp. 1225–1229. Weinheim: Verlag Chemie 1970
Gogotov, J. N., Schlegel, H. G.: N2-fixation by chemoautotrophic hydrogen bacteria. Arch. Microbiol. 97, 359–362 (1974)
Harada, T.: Succinoglucan and gel-forming Beta-1,3-Glucan. Proc. Biochem. 9, 21–25 (1974)
Kurz, G., Wallenfels, K.: d-Galactose: UV Test mit Galactose-Dehydrogenase. In: Methoden der enzymatischen Analyse, H. U. Bergmeyer, Bd. II, pp. 1241–1244. Weinheim: Verlag Chemie 1970
Leisinger, Th., Wiemken, A., Ettlinger, L.: Über cellulosefreie Mutanten von Acetobacter xylinus. Arch. Mikrobiol. 54, 21–36 (1966)
Lowry, C. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with folin-phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Roe, J. H.: Colorimetric method for the determination of fructose in blood and urine. J. biol. Chem. 107, 15–22 (1934)
Schlegel, H. G., Kaltwasser, H., Gottschalk, G.: Ein Submersverfahren zur Kultur wasserstoffoxydierender Bakterien: Wachstumsphysiologische Untersuchungen. Arch. Mikrobiol. 38, 209–222 (1961)
Senior, P. J., Beech, G. A., Ritchie, F. A. F., Dawes, E. A.: The role of oxygen limitation in the formation of poly-β-hydroxybutyrate during batch and continuous culture of Azotobacter beijerinckii. Biochem. J. 128, 1193–1201 (1972)
Siebert, D.: Über propanverwertende, wasserstoffoxidierende Bakterien und die Charakterisierung eines Förderungsfaktors. Dissertation, Universität Göttingen (1969)
Somogyi, M.: Notes on sugar determination. J. biol. Chem. 195, 19–23 (1952)
Trevelyan, W. E., Harrison, J. S.: Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem. J. 50, 298–303 (1952)
Tunail, N.: Charakterisierung des Wasserstoffbacteriums Arthrobacter 7 C und der Regulation des Kohlenhydratstoffwechels. Dissertation, Universität Göttingen (1973)
Tunail, N., Schlegel, H. G.: Phosphoenolpyruvate, a new inhibitor of glucose-6-phosphate dehydrogenase. Biochem. biophys. Res. Commun. 49, 1554–1560 (1972)
Tunail, N., Schlegel, H. G.: A new coryneform hydrogen bacterium: Corynebacterium autotrophicum strain 7 C. I. Characterization of the wild type strain. Arch. Microbiol. 100, 341–350 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Andreesen, M., Schlegel, H.G. A new coryneform hydrogen bacterium: Corynebacterium autotrophicum strain 7 C . Arch. Microbiol. 100, 351–361 (1974). https://doi.org/10.1007/BF00446328
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00446328