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Microbacterium kunmingensis sp. nov., an attached bacterium of Microcystis aeruginosa

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Abstract

A Gram-stain positive, aerobic, rod-shaped actinobacterial strain designated as JXJ CY 27-2T was isolated from the culture of Microcystis aeruginosa FACHB-905 (Maf) collected from Lake Kunming, southwest China. The isolate was catalase positive, oxidase negative, and able to grow at 10.0-44.0 °C, pH 5.0-10.0 and 0–5.0% NaCl. Based on the 16S rRNA gene sequences, JXJ CY 27-2T showed high similarities of 98.54–98.55% with Microbacterium invictum DSM 19600T, Microbacterium saccharophilum DSM 28107T, and Microbacterium aoyamense DSM 19461T, and less than 98.47% similarities with other members of the genus. Its major cellular fatty acids were anteiso-C17:0 and anteiso-C15:0. The predominant menaquinones were MK-11 and MK-12. The diagnostic diamino acid in the cell wall peptidoglycan was lysine. Whole cell sugars contained mannose, ribose, galactose, rhamnose and arabinose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids, and an unidentified lipid. The DNA G + C content was 69.8%. The digital DNA-DNA hybridization and average nucleotide identity values between strain JXJ CY 27-2T and its three closest similar strains were 18.4–20.3% and 74.9–75.7%, respectively. Based on the above data, strain JXJ CY 27-2T was identified as a new species of the genus Microbacterium, for which the name Microbacterium kunmingensis sp. nov. is proposed. The type strain is JXJ CY 27-2T (=CGMCC 1.17506T = KCTC 49382T). Strain JXJ CY 27-2T could promote the growth of Maf by providing it with available phosphorus, nitrogen and probably other nutrients such as vitamins and indole-3-acetate.

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Acknowledgements

This research was supported by funds from Natural Science Foundation of China (No. 31060010).

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  1. These authors contributed equally: Yao Xiao, Min Chen

Authors and Affiliations

  1. College of Pharmacy and Life Science, Jiujiang University, Jiujiang, 332000, China

    Yao Xiao, Min Chen, Jian Chen, Li-Na Mao, Yi-Ru Peng, Shan-Shan Gui & Bing-Huo Zhang

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Contributions

BZ designed the experiments; YX, MC, JC, LM, YP and SG performed the experiments; YX, MC and BZ analyzed the data; YX and MC drafted the manuscript and BZ revised the manuscript.

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Correspondence to Bing-Huo Zhang.

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Xiao, Y., Chen, M., Chen, J. et al. Microbacterium kunmingensis sp. nov., an attached bacterium of Microcystis aeruginosa. J Antibiot 75, 662–670 (2022). https://doi.org/10.1038/s41429-022-00568-w

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