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Production of lipase free of citrinin by Penicillium citrinum

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Abstract

Lipase (Glycerol ester hydrolase E.G. 3.1.1.3) from a Brazilian strain of Penicillium citrinum free of the mycotoxin citrinin has been investigated. Citrinin production was inhibited by using culture medium containing olive oil, soybean oil and corn oil as carbon sources. Potassium concentration and pH play an important role in citrinin production. Potassium concentration lower than 30 mM and pH below 4.5 inhibited the mycotoxin production. P. citrinum produced lipase free of extraneous proteins and citrinin when cultured using, as nitrogen source, ammonium sulphate (lipase activity of 7.88 U/mg) and yeast extract (lipase activity of 4.95 U/mg) with olive oil as carbon source. This data is relevant to the larger scale production of lipases for food technology applications, from Penicillium citrinum.

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Authors and Affiliations

  1. Department of Biochemistry, Immunopathology Keizo Asami Laboratory, LIKA, Universidade Federal de Pernambuco, CEP 50870-901, Recife PE, Brazil

    M. C. B. Pimentel, E. H. M. Melo & J. L. Lima Filho

  2. Instituto de Química, Biological Chemistry Laboratory, Universidade Estadual de Campinas, C.P. 6154 Campinas, CEP 13083-970, SP, Brazil

    N. Durán

Authors
  1. M. C. B. Pimentel
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  2. E. H. M. Melo
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  3. J. L. Lima Filho
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  4. N. Durán
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Pimentel, M.C.B., Melo, E.H.M., Lima Filho, J.L. et al. Production of lipase free of citrinin by Penicillium citrinum . Mycopathologia 133, 119–121 (1996). https://doi.org/10.1007/BF00439123

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  • DOI: https://doi.org/10.1007/BF00439123

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