Summary
An easy and sensitive way of measuring the proportion of E. coli cells which are lysogenized by lambda phage or lambda mutants has been devised. With this assay it was possible to analyse the lysogenic response as a function of the average phage input per cell. The results indicate that lysogenization of exponentially growing cells requires that the cells are infected by at least two phages able to replicate, or three or four phages unable to replicate.
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References
Appleyard, R. K.: Segregation of lambda lysogenicity during bacterial recombination in E. coli K12. Genetics 39, 429 (1954).
Brooks, K.: Studies in the physiological genetics of some suppressor sensitive mutants of bacteriophage lambda. Virology 26, 489 (1965).
Campbell, A.: Sensitive mutants of bacteriophage lambda. Virology 14, 22 (1961).
Dove, W. F.: The action of the lambda chromosome. I. The control of functions late in phage development. J. molec. Biol. 19, 187 (1966).
Echols, H.: Developmental pathways for the temperate phage: lysis versus lysogeny. Ann. Rev. Genet. (1972) (in the press).
Fry, B. A.: Conditions for the infection of the E. coli lambda phage and for the establishment of lysogeny. J. gen. Microbiol. 21, 676 (1959).
Fry, B. A., Gros, F.: The metabolic activities of E. coli during the establishment of lysogeny. J. gen. Microbiol. 21, 685 (1959).
Gottesman, M. E., Weisberg, R. A.: Prophage insertion and excision. In the bacteriophage lambda: p. 113 (A. D. Hershey, editor). Cold Spring Harbor 1971.
Grodzicker, T., Arditti, R. R., Eisen, H.: Establishment of repression by lambdoid phage in catabolic activator protein and adenylate cyclase mutants of E. coli. Proc. nat. Acad. Sci. (Wash.) 69, 366 (1972).
Hershey, A. D., Rotman, R.: Genetic recombination between host range and plaque type mutants of bacteriophage in single bacterial cells. Genetics 34, 44 (1949).
Hoffman, D. B., Rubenstein, I.: Physical studies of lysogeny. I. Properties of intracellular parental bacteriophage DNA from lambda infected sensitive bacteria. J. molec. Biol. 35, 375 (1968).
Kaiser, A. D.: Mutations in a temperate phage affecting its ability to lysogenize. Virology 3, 42 (1957).
Kellenberger, G., Zichichi, M., Weigle, J.: A mutation affecting the DNA content of bacteriophage lambda and its lysogening properties. J. molec. Biol. 3, 399 (1961).
Kourilsky, P.: Lysogenization by bacteriophage lambda and the regulation of repressor synthesis. Virology 45, 853 (1971).
Lieb, M.: The establishment of lysogenicity in E. coli. J. Bact. 65, 642 (1953).
Mackman, R. S., Sutherland, E. W.: Adenosine 3′–5′ phosphate in E. coli., J. biol. Chem. 240, 1309 (1965).
Ogawa, T., Tomizawa, J.: Replication of bacteriophage lambda DNA. I. Replication of DNA of lambda phage defective in early functions. J. molec. Biol. 38, 217 (1968).
Parkinson, J. S.: Deletion mutants of bacteriophage lambda. II. Genetic properties of att-defective mutants. J. molec. Biol. 56, 385 (1971).
Parkinson, J. S., Huskey, R.: Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization. J. molec. Biol. 56, 369 (1971).
Signer, E.: Plasmid formation: a new mode of lysogeny by phage lambda. Nature (Lond.) 223, 158 (1969).
Sussman, R., Jacob, F.: Sur un systéme de répression thermosensible chez le bactériophage lambda. C. R. Acad. Sci. (Paris) 254, 1517 (1962).
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Communicated by R. Devoret
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Kourilsky, P. Lysogenization by bacteriophage lambda. Molec. Gen. Genet. 122, 183–195 (1973). https://doi.org/10.1007/BF00435190
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DOI: https://doi.org/10.1007/BF00435190