Summary
The fine structural localization of acid phosphatase in the different cells in a benign giant cell tumor of bone has been studied. Stromal cells type 1 and 2 (fibroblast-like and macrophage-like, respectively) showed the presence of lead phosphate precipitate following incubation in a Gomori-type lead salt medium only in conventional lysosomes. In the multinucleated giant cells, the final product was deposited over lysosome-like organelles, and also over Golgi cisternae, vesicles, and vacuoles. Furthermore, evidence for presence of acid phosphatase was obtained in smooth-surfaced tubular, sausage-, horse-shoe-, and ring-shaped structures and over digestive vacuoles of autophagic or heterophagic origin. Finally, in these cells, many of the tubular and vacuolar elements located subjacent to areas of the plasma membrane with microvillous specializations (abortive brush borders?) were shown to carry acid phosphatase.
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Aparisi, T., Arborgh, B., Ericsson, J.L.E.: Giant cell tumor of bone. Detailed fine structural analysis of different cell components. Virchows Arch. A. Path. Anat. and Histol. 376, 273–298 (1977)
Arborgh, B., Ericsson, J.L.E., Helminen, H.: Inhibition of renal acid phosphatase and aryl sulfatase activity by glutaraldehyde fixation. J. Histochem. Cytochem. 19, 449–451 (1971)
Berthet, J., de Duve, C.: Tissue fractionation studies. I. The existence of a mitochondria-linked, enzymically inactive form of acid phosphatase in rat liver tissue. Biochem. J. 50, 174 (1952)
Bowers, W.E., Finkenstaedt, I.T., de Duve, C.: Lysosomes in lymphoid tissue. I. The measurement of hydrolytic activities in whole homogenates. J. Cell Biol. 32, 325–337 (1967)
Essner, E.: Phosphatases. In: Electron Microscopy of Enzymes, (M.A. Hayat, Ed.). pp. 44–76. New York: van Noghaud Reinhold Co. 1973
Göthlin, G., Ericsson, J.L.E.: Fine structure localization of acid phosphomonesterase in the osteoblasts and osteocytes of fracture callus. Histochemie 35, 81–91 (1973a)
Göthlin, G., Ericsson, J.L.E.: Studies on the ultrastructural localization of adenosine triphosphatase activity in the fracture callus. Histochemie 35, 111–126 (1973b)
Göthlin, G., Ericsson, J.L.E.: Fine structural localization of alkaline phosphatase in the fracture callus of the rat. Histochemie 36, 225–236 (1973c)
Hamberg, H., Brunk, U., Ericsson, J.L.E., Jung, B.: Cytoplasmic effects of X-irradiation on cultured cells in a non-dividing stage. II. Alterations in lysosomes, plasma membrane, Golgi apparatus, and related structures. Acta Path. Microbiol. Scand. Section A Pathology (1977) (in press)
Helminen, H., Ericsson, J.L.E.: On the mechanisms of lysosomal enzyme secretion. Electron microscopic and histochemical studies on epithelial cells of the rat's ventral prostate lobe. J. Ultrastruct. Res. 33, 528–549 (1970)
Jeffree, G.M., Price, C.H.G.: Bone tumours and their enzymes. Jour. of Bone and Joint Surgery, 47-B, 123–136 (1965)
Oliva, H., Navas, J., Navarro, V., Ferrer, M.: Histogenesis ultrastructural de un tumor de celulas gigantes recidivantes de hueso. Rev. Ortoped. Traum. 18, 3–18 (1974)
Ores, R., Rosen, P., Ortiz, J.: Localization of acid phosphatase activity in a giant cell tumor of bone. Arch. Path. 88, 54–57 (1969)
Schajowicz, F.: Giant cell tumor of bone. A histochemical study. J. Bone Joint Surg. 43 A, 1–29 (1961)
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Aparisi, T., Arborgh, B. & Ericsson, J.L.E. Giant cell tumor of bone. Virchows Arch. A Path. Anat. and Histol. 376, 299–308 (1977). https://doi.org/10.1007/BF00432300
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DOI: https://doi.org/10.1007/BF00432300