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A diagnosis of giant cell-rich tumour of bone is supported by p63 immunohistochemistry, when more than 50 % of cells is stained

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Abstract

Some primary malignant or benign tumours of bone contain numerous multinucleated cells. These “giant cell-rich tumours of bone” have overlapping features and clinical and radiological data are needed to reach an accurate pathological diagnosis. We studied the potential contribution of p63 immunohistochemistry to the reliability of the histological diagnosis. We performed a multicentric retrospective study of 291 giant cell-rich tumours of bone which included 119 giant cell tumours of bone (GCTB), 76 aneurysmal bone cysts (ABC), 49 chondroblastomas (CB), 15 nonossifying fibromas (NOF), 10 giant cell reparative granulomas (RG) of jaws, 1 giant cell lesion of small bones, 2 hyperparathyroidism-related brown tumours (BT), 17 bone sarcomas with numerous osteoclasts and 2 malignant giant cell tumours of bone. p63 is expressed in ABC, CB, NOF, RG, BT and GCTB, but its expression in more than 50 % of mononuclear cells is strongly suggestive of a diagnosis of GCTB. In contrast, malignant GCTB were mostly negative. Our results show that p63 is expressed in a broad range of benign giant cell-rich tumours of bone, consistent with data in the recent literature, while infrequent in malignant tumours. With a cut-off 50 %, the presence of p63 positive cells is useful in supporting a diagnosis of giant cell-rich tumour of bone. However, a final diagnosis cannot be made without due consideration of all clinical/radiological and pathological data.

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Acknowledgments

The tumour specimens in Marseille were retrieved from the pathological files of the APHM Biobank (authorized number AC-2013-1786).

This work was supported by institutional grants from the Department of Pathology of the Timone Hospital and UMR 911 (AMU).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Service d’Anatomie Pathologique, Hôpital la Timone, UMR911 AMU, Marseille, France

    André Maues De Paula

  2. Groupement hospitalier EST, Centre de Pathologie et Neuropathologie EST, Bron, France

    Alexandre Vasiljevic & Corinne Bouvier

  3. Service de Santé Publique et d’Information Médicale, Hôpital la Timone, Marseille, France

    Roch Giorgi

  4. Service d’Anatomie Pathologique, Hôpital Rangueil, Toulouse, France

    Anne Gomez-Brouchet

  5. Pôle Biologie-Pathologie, parc Eurasanté, CHRU, Lille, France

    Sébastien Aubert & Xavier Leroy

  6. CHU Pontchaillou, Département d’Anatomie et de Cytologie Pathologiques, Rennes, France

    Hélène Duval

  7. Service d’Anatomie Pathologique, Hôpital Trousseau, Tours, France

    Gonzague de Pinieux

  8. Service d’Anatomie Pathologique et Neuropathologie du Pr Figarella-Branger, Centre Hospitalier Universitaire La Timone, 264 Rue Saint Pierre, 13005, Marseille, CEDEX 05, France

    André Maues De Paula

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  1. André Maues De Paula
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  2. Alexandre Vasiljevic
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Correspondence to André Maues De Paula.

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André Maues De Paula and Alexandre Vasiljevic equally contributed to the work

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Maues De Paula, A., Vasiljevic, A., Giorgi, R. et al. A diagnosis of giant cell-rich tumour of bone is supported by p63 immunohistochemistry, when more than 50 % of cells is stained. Virchows Arch 465, 487–494 (2014). https://doi.org/10.1007/s00428-014-1637-z

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  • DOI: https://doi.org/10.1007/s00428-014-1637-z

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