Abstract
One of the obligate thermophilic bacteria, Bacillus stearothermophilus, was unable to grow at temperatures below 35° C. About 80% of the population in the bacterial culture died at the temperatures, and the same extent of loss in either of the activities of oxygen consumption or synthesis of protein or nucleic acid of the organisms was observed. With the progress of death of the organisms, reduced nicotinamide-adenine dinucleotide came to be oxidized by the organisms, enzymes such as fructose-1,6-diphosphate aldolase, when the organisms were washed with phosphate buffer, were leaked out of the organisms, and an increasing amount of ribonucleoprotein was released into the culture medium. The change of the membrane state was then suggested to be one of the possible causes for the death of the organisms at the temperatures.
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References
Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315–323 (1956)
Garen, A., Levinthal, C.: A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochim. biophys. Acta (Amst.) 38, 470–483 (1960)
Gornall, A. G., Bardawill, C. J., Davis, M. M.: Determination of serum proteins by means of the biuret reaction. J. biol. Chem. 177, 751–766 (1949)
Hachimori, A., Muramatsu, N., Nosoh, Y.: Studies of an ATPase of thermophilic bacteria. I. Purification and properties. Biochim. biophys. Acta (Amst.) 206, 426–437 (1970)
Hirano, J., Mutoh, Y., Kitamura, M., Asai, S., Nakajima, I., Takamiya, A.: Effect of change of temperature upon the viability of a thermophilic bacterium. J. gen. appl. Microbiol. 4, 188–199 (1958)
Ingraham, J. L.: Temperature relationship. In: The bacteria, Vol. 4, I. G. Gunsalus, R. Y. Stanier, eds., pp. 265–296. New York-London: Academic Press 1962
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Mejbaum, W.: Estimation of small amounts of pentose especially in derivatives of adenylic acid. Hoppe-Seylers Z. physiol. Chem. 258, 117–120 (1939)
Muramatsu, N., Nosoh, Y.: Purification and characterization of glucose-6-phosphate isomerase from Bacillus stearothermophilus. Arch. Biochem. Biophys. 144, 245–252 (1971)
Oshima, M., Miyagawa, A.: Comparative studies of the fatty acid composition on moderate and extremely thermophilic bacteria. Lipids 9, 476–480 (1974)
Singer, S. J., Nicolson, G. L.: The fluid mosaic model of the structure of cell membranes. Science 175, 720–731 (1972)
Sugimoto, S., Nosoh, Y.: Thermal properties of fructose-1,6-diphosphare aldolase from thermophilic bacteria. Biochim. biophys. Acta (Amst.) 235, 210–221 (1971)
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Hashizume, S., Sekiguchi, T. & Nosoh, Y. Effect of temperature on the viability of Bacillus stearothermophilus . Arch. Microbiol. 107, 75–80 (1976). https://doi.org/10.1007/BF00427870
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DOI: https://doi.org/10.1007/BF00427870