Abstract
The dehydrogenase activity of Escherichia coli BB cell extracts was studied at different growth stages in the presence of different substrates and triphenyl tetrazolium chloride as an electron acceptor. It was shown that the highest degree of reduction of triphenyl tetrazolium chloride was observed during exponential growth of the bacteria when potassium isocitrate was used as a substrate. It was found that extracts of the bacteria during the exponential phase of growth on an inert glassy carbon electrode in a three-electrode liquid electrochemical cell manifested electrochemical activity in the presence of potassium citrate and methylene blue or potassium hexacyanoferrate(III) as redox mediators.
Similar content being viewed by others
References
Barton, C., Gallaway, S., and Atanassov, J., Chem. Rev., 2004, vol. 104, no. 10, pp. 4867–4886.
Kim, J., Jia, H., and Wang, P., Biotechnol. Adv., 2006, vol. 24, no. 3, pp. 296–308.
Willner, I., Yan, Y.M., Willner, B., and TelVered, R., Fuel Cells, 2009, vol. 9, no. 1, pp. 7–24.
Morozov, S.V., Karyakina, E.E., Zorin, N.A., Varfolomeyev, S.D., Cosnier, S., and Karyakina, A.A., Bioelectrochem. Bioenerg., 2002, vol. 55, no. 1-2, pp. 169–171.
Karyakin, A.A., Vinogradova, D.V., Morozov, S.V., and Karyakina, E.E., Electrochim. Acta, 2009, vol. 55, no. 26, pp. 7696–7700.
Kuwahara, T., Yamazaki, H., Kondo, M., and Shimomura, M., Appl. Surf. Sci., 2012, vol. 258, no. 17, pp. 6321–6325.
Milton, R.D., Lim, K., Hickey, D.P., and Minteer, S.D., Bioelectrochem. Bioenerg., 2015, vol. 106, pt. A, pp. 56–63.
Osadebe, I., Conghaile, P.O., Kavanagh, P., and Leech, D., Electrochim. Acta, 2015, vol. 182, pp. 320–326.
Scherbahn, V., Putze, M.T., Dietzel, B., Heinlein, T., Schneider, J.J., and Lisdat, F., Biosens. Bioelectron, 2014, vol. 61, pp. 631–638.
Zhu, Z., Sun, F., Zhang, X., and Zhang, Y.-H.P., Biosens. Bioelectron., 2012, vol. 36, no. 1, pp. 110–115.
Neto, S.A., Suda, E.L., Xu, S., Meredith, M.T., De Andrade, A.R., and Minteer, S.D., Electrochim. Acta, 2012, vol. 87, pp. 323–329.
Neto, S.A., Forti, J.C., Zucolotto, V., Ciancaglini, P., and De Andrade, A.R., Biosens. Bioelectron., 2011, vol. 26, no. 6, pp. 2922–2926.
Gutiérrez-Domínguez, D.E., Pacheco-Catalán, D.E., Patiño-Diaz, R., Canto-Canché, B., and Smit, M.A., Int. J. Hydrogen Energy, 2012, vol. 38, no. 28, pp. 12610–12616.
Neto, S.A., Suda, E.L., Xu, S., Meredith, M.T., De Andrade, A.R., and Minteer, S.D., Electrochim. Acta, 2012, vol. 87, pp. 323–329.
Zhang, X., Ranta, A., and Halme, A., Biosens. Bioelectron., 2006, vol. 21, no. 11, pp. 2052–2057.
Das, M., Barbora, L., Das, P., and Goswami, P., Biosens. Bioelectron., 2014, vol. 59, pp. 184–191.
Stoica, L., Ruzgas, T., Ludwig, R., Haltrich, D., and Gorton, L., Langmuir, 2006, vol. 22, no. 25, pp. 10801–10806.
Kuz’micheva, E.V., Reshetov, V.A., Kazarinov, I.A., and Ignatov, O.V., Elektrokhim. Energet., 2007, vol. 7, no. 1, pp. 33–37.
Nandy, A., Kumar, V., and Kundu, P.P., Biosens. Bioelectron., 2016, vol. 79, pp. 796–801.
Miroliaei, M.R., Samimi, A., Mohebbi-Kalhori, D., and Khorram, M., J. Ind. Eng. Chem., 2014, vol. 25, pp. 42–50.
Harreither, W., Coman, V., Ludwig, R., Haltrich, D., and Gorton, L., Electroanalysis, 2007, vol. 19, nos. 2–3, pp. 172–180.
Babkina, E., Chigrinova, E., Ponamoreva, O., Alferov, V., and Reshetilov, A., Electroanalysis, 2006, vol. 18, nos. 19–20, pp. 2023–2029.
Ramanavicius, A., Habermüller, K., Csöregi, E., Laurinavicius, V., and Schuhmann, W., Anal. Chim., 1999, vol. 71, no. 16, pp. 3581–3586.
Trashin, S.A., Haltrich, D., Ludwig, R., Gorton, L., and Karyakin, A.A., Bioelectrochem. Bioenerg., 2009, vol. 76, nos. 1–2, pp. 87–92.
Neto, S.A., Hickey, D.P., Milton, R.D., De Andrade, A.R., Shelley, D., and Minteer, S.D., Biosens. Bioelectron., 2015, vol. 72, pp. 247–254.
Karyakin, A.A., Ivanova, Y.N., and Karyakina, E.E., Bioelectrochem. Bioenerg., 2003, vol. 5, no. 8, pp. 677–680.
Shcherbakov, V.P. and Kudryashova, E.A., DNA Repair, 2014, vol. 21, pp. 120–130.
Brown, R.E., Jarvis, K.L., and Hyland, K.J., Anal. Biochem., 1989, vol. 180, no. 1, pp. 136–139.
Pleshkov, B.P., Praktikum po biokhimii rastenii (A Practical Course in Plant Biochemistry), Moscow Kolos, 1976.
Tsyperovich, A.S., Fermenty (Enzymes), Kiev Tekhnika, 1971.
Impert, O., Katafias, A., Kita, P., Mills, A., Pietkiewicz-Graczyk, A., and Wrzeszcz, G., Dalton Trans., 2003, no. 3, pp. 348–353.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © M.V. Dmitrieva, E.V. Zolotukhina, E.V. Gerasimova, A.A. Terent’ev, Yu.A. Dobrovol’skii, 2017, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 4, pp. 406–412.
Rights and permissions
About this article
Cite this article
Dmitrieva, M.V., Zolotukhina, E.V., Gerasimova, E.V. et al. Dehydrogenase and electrochemical activity of Escherichia coli extracts. Appl Biochem Microbiol 53, 458–463 (2017). https://doi.org/10.1134/S0003683817040032
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683817040032