Summary
Genetic analysis of histidine independent (His4) revertants induced by ultraviolet light in the his-4 E. coli strain AB1157 was carried out: 83% carried ochre (UAA) suppressor mutations and 17% carried back mutations to his + or (intragenic?) suppressors not detectably separable from his-4. Using the specialized transducing λpsu 2int − phage, which carries supE-supB, it was determined that 87% of the ochre suppressors mapped in the supE-supB region. We were able to deduce that 56% of these affected tRNA Gln1 by a CAA→TAA change in the tRNA gene while 31% affected tRNA Gln2 by TAG→TAA change. Although we were unable to deduce the base substitution of the remaining 13%, the results indicated that most of the suppressor mutations are caused by a G:C to A:T transition.
These results suggest that the high incidence of supE-supB region suppressor mutation in E. coli by UV would be a reflection of the general feature of UV mutagenesis; i.e. preferential induction of G:C to A:T transition in repairing nonparing DNA lesions.
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Communicated by B.A. Bridges
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Kato, T., Shinoura, Y., Templin, A. et al. Analysis of ultraviolet light-induced suppressor mutations in the strain of Escherichia coli K-12 AB1157: An implication for molecular mechanisms of UV mutagenesis. Molec. Gen. Genet. 180, 283–291 (1980). https://doi.org/10.1007/BF00425840
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DOI: https://doi.org/10.1007/BF00425840