Summary
The transcriptional organization and sequence of the Aspergillus nidulans argB gene, encoding ornithine carbamoyl transferase (OCTase; E.C. 2.1.3.3.), was determined. Transcription of the gene begins within a methionine-initiated open translation reading frame, indicating that a second methionine codon of the open reading frame is used for translation initiation. The predicted length of the OCTase precursor peptide is 359 amino acids, and it contains a highly basic amino terminus that is probably involved in mitochondrial targeting. There is extensive homology between Aspergillus OCTase and mammalian and bacterial OCTases and weaker homology between the Aspergillus polypeptide and bacterial arginine carbamoyl transferase.
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Benoist C, O'Hare K, Breathnach R, Chambon P (1980) Nucleic Acids Res 8:27–142
Berget S (1984) Nature 309:179–182
Berse B, Dmochowska A, Skrzypek M, Weglenski P, Bates MA, Weiss RJ (1983) Gene 25:107–117
Boel E, Hjort I, Svensson B, Borris K, Norris FE, Fiil NP (1984) EMBO J 3:1097–1102
Buxton FP, Gwynne DI, Davies RW (1985) Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans. Gene 37:207–214
Clewell DB, Helinski DR (1969) Proc Natl Acad Sci USA 62:1159–1166
Corden J, Wasylyk B, Buchwalder A, Sassone-Corsi P, Kedinger C, Chambon P (1980) Science 209:1406–1414
Crabeel M, Huygen R, Cunin R, Glansdorff N (1983) EMBO J 2:205–212
Davis RH (1983) Arginine synthesis in eukaryotes in amino acids — biosynthesis and genetic regulation. In: Herrmann K, Somerville R (eds) Adison-Wesley, Reading, Mass, pp 81–101
Horwich AL, Fenton WA, Williams KR, Kalousek F, Kraus JP, Doolittle RF, Konigsberg W, Rosenberg LE (1984) Science 224:1068–1074
Hu N, Messing J (1982) Gene 17:271–277
Innes MA, Holland MJ, McCabe PC, Cole GE, Wittman VP, Tal R, Watt WK, Gelfand DH, Holland JP, Meade JH (1985) Science 228:21–26
John MA, Peberdy J (1984) Enzyme and microbial technology 6:386–389
Johnstone IL, Hughes SG, Clutterbuck AJ (1985) EMBO J 5:1307–1311
Kinnaird JH, Fincham JRS (1983) Gene 26:253–260
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbour Laboratory, Cold Spring Harbour, New York, p 86
McClauchlen JD, Gaffney D, Whitton JL, Clements JB (1985) Nucleic Acids Res 13:1347–1368
McIntyre P, Graf L, Mercer JFB, Wake SA, Hudson P, Hoogenraad N (1985) DNA 4:147–156
McKnight GL, Kato H, Upshall A, Parker MD, Saari G, O'Hara PJ (1985) EMBO J 4:2093–2099
Messing J (1983) Enzymol 100B:20–78
Miller BL, Miller KY, Timberlake WE (1985) Mol Cell Biol 5:1714–1721
Monaco HL, Crawford JL, Lipscomb WN (1978) Proc Natl Acad Sci USA 75:5276–5280
Mullaney EJ, Hamer JE, Roberti KA, Yelton MM, Timberlake WE (1985) Mol Gen Genet 199:37–45
Nunberg JH, Meade JH, Cole G, Lawyer FC, McCabe P, Schweikhardt V, Tal R, Wittman VP, Flatgaard JE, Innes MA (1984) Mol Cell Biol 11:2306–2315
Proudfoot NJ, Brownlee GG (1976) Nature 263:211–214
Rigby PWJ, Dieckman M, Rhodes C, Berg P (1977) J Mol Biol 113:237–251
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schechtman MG, Yanofsky C (1983) J Molec Appl Genet 2:83–99
Shoemaker S, Schweikhardt V, Ladner M, Gelfand D, Kwok S, Myambo K, Innes MA (1983) Biotechnology 1:691–696
Timberlake WE, Barnard EL (1981) Cell 26:29–37
Upshall A (1986) Curr Genet 10:593–599
van Vliet F, Cunin R, Jacobs A, Piette J, Gigot D, Lauwereys M, Pierard A, Glansdorff N (1984) Nucleic Acids Res 12:6277–6289
Ward M, Wilkinson B, Turner G (1986) Mol Gen Genet 202:265–270
Weiss RL, Puetz D, Cybis J (1985) Expression of Aspergillus genes in Neurospona in gene manipulations in fungi. In: Bennett, Lasure (eds) Academic Press, New York, pp 280–291
Yelton ME, Hamer JE, Timberlake WE (1984) Proc Natl Acad Sci USA 81:1470–1474
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Communicated by R.B. Goldberg
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Upshall, A., Gilbert, T., Saari, G. et al. Molecular analysis of the argB gene of Aspergillus nidulans . Molec. Gen. Genet. 204, 349–354 (1986). https://doi.org/10.1007/BF00425521
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DOI: https://doi.org/10.1007/BF00425521