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The coupling effects of some thiol and other sulfur-containing compounds on the circadian rhythm of cell division in photosynthetic mutants of Euglena

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Abstract

Previous work has demonstrated a persisting, free-running, circadian rhythm of cell division in the P4ZUL photosynthetic mutant of the alga Euglena gracilis Klebs (Strain Z) Pringsheim grown organotrophically in continuous light or darkness at 19° C following prior synchronization by a repetitive LD: 10,14 light cycle. A similar circadian rhythmicity has been recently discovered in the W6ZHL heat-bleached and the Y9ZNalL naladixic acid-induced mutants of Euglena grown under comparable conditions. Over extended timespans, however, these mutants appear to gradually lose first their ability to display persisting overt rhythms, and then even their capability of being entrained by imposed LD cycles. These properties can be restored by the addition of certain sulfur-containing compounds to the medium including cysteine, methionine, dithiothreital, sodium monosulfide, sodium sulfite, and sodium thiosulfate, as well as thioglycolic [mercaptoacetic] acid. The implications of these findings toward biological clock mechanisms are discussed: It appears that some sort of coupling process is operating as opposed to the initiation of an underlying oscillation.

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Abbreviations

LL:

continuous illumination

DD:

continuous darkness

LD:

repetitive light-dark cycle

SS:

stepsize

τ:

period of biological rhythm

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Authors and Affiliations

  1. Department of Cellular and Comparative Biology, Division of Biological Sciences, State University of New York, 11794, Stony Brook, New York, USA

    Leland N. Edmunds Jr., M. E. Jay, A. Kohlmann, S. C. Liu, V. H. Merriam & H. Sternberg

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  1. Leland N. Edmunds Jr.
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  2. M. E. Jay
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  3. A. Kohlmann
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  4. S. C. Liu
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  5. V. H. Merriam
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  6. H. Sternberg
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Supported by research grants (GB-36287, GB-43543) from the National Science Foundation.

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Edmunds, L.N., Jay, M.E., Kohlmann, A. et al. The coupling effects of some thiol and other sulfur-containing compounds on the circadian rhythm of cell division in photosynthetic mutants of Euglena . Arch. Microbiol. 108, 1–8 (1976). https://doi.org/10.1007/BF00425086

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  • DOI: https://doi.org/10.1007/BF00425086

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