Abstract
Nitrosomonas europaea and Nitrosovibrio sp. produced NO and N2O during nitrification of ammonium. Less then 15% of the produced NO was due to chemical decomposition of nitrite. Production of NO and especially of N2O increased when the bacteria were incubated under anaerobic conditions at decreasing flow rates of air, or at increasing cell densities. Low concentrations of chlorite (10 μM) inhibited the production of NO and N2, but not of nitrite indicating that NO and N2O were not produced during the oxidative conversion of ammonium to nitrite. NO and N2O were produced during reduction of nitrite with hydrazine as electron donor in almost stoichiometric quantities indicating that reduction of nitrite was the main source of NO and N2O.
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References
Aleem MIH, Lees H (1963) Autotrophic enzyme systems. I. Electron transport systems concerned with hydroxylamine oxidation in Nitrosomonas. Can J Biochem Physiol 41:763–778
Anderson IC, Levine JS (1986) Relative rates of nitric oxide and nitrous oxide production by nitrifiers, denitrifiers, and nitrate respirers. Appl Environ Microbiol 51:938–945
Anderson JH (1964) Estimation of nitric oxide formed from hydroxylamine by Nitrosomonas. Biochim Biophys Acta 97:337–339
Baumgärtner M, Remde A, Bock E, Conrad R (1990) Release of nitric oxide from building stones into the atmosphere. Atmos Environ 24B:87–92
Betlach MR, Tiedje JM (1981) Kinetic explanation for accumulation of nitrite, nitric oxide, and nitrous oxide during bacterial denitrification. Appl Environ Microbiol 42:1074–1084
Chalk PM, Smith CJ (1983) Chemodenitrification. Dev Plant Soil Sci 9:65–89
Conrad R (1990) Flux of NOx between soil and atmosphere: importance and soil microbial metabolism. In: Soerensen J, Revsbech NP (eds) Denitrification in soil and sediment. Plenum Press, New York
Conrad R, Seiler W (1980) Field measurements of the loss of fertilizer nitrogen into the atmosphere as nitrous oxide. Atmos Environ 14:555–558
Crutzen PJ (1979) The role of NO and NO2 in the chemistry of the troposphere and stratosphere. Annu Rev Earth Planet Sci 7:443–472
DiSpirito AA, Taafe LR, Lipscomb JD, Hooper AB (1985) A “blue” copper oxidase from Nitrosomonas europaea. Biochim Biophys Acta 827:320–326
Ehhalt DM, Drummond JW (1982) The tropospheric cycle of NOx. In: Georgii HW, Jaeschke W (eds) Chemistry of the unpolluted and polluted troposphere. Reidel, Dordrecht, pp 219–251
Firestone MK, Davidson EA (1989) Microbiological basis of NO and N2O production and consumption. In: Andreae MO, Schimel DS (eds) Exchange of trace gases between terrestrial ecosystems and the atmosphere. Dahlem Konferenzen. Wiley, Chichester, pp 7–21
Goreau TJ, Kaplan WA, Wofsy SC, McElroy MB, Valois FW, Watson SW (1980) Production of NO sup-inf2 and N2O by nitrifying bacteria at reduced concentrations of oxygen. Appl Environ Microbiol 40:526–532
Hooper AB (1968) A nitrite-reducing enzyme from Nitrosomonas europaea. Preliminary characterization with hydroxylamine as electron donor. Biochim Biophys Acta 162:49–65
Hooper AB (1989) Biochemistry of the nitrifying lithoautotrophic bacteria. In: Schlegel HG, Bowien B (eds) Autotrophic bacteria. Science Tech Publishers, Madison, pp 239–265
Hooper AB, Terry KR (1979) Hydroxylamine oxidoreductase of Nitrosomonas. Production of nitric oxide from hydroxylamine. Biochim Biophys Acta 571:12–20
Hynes RK, Knowles R (1983) Inhibition of chemoautotrophic nitrification by sodium chlorate and sodium chlorite: a reexamination. Appl Environ Microbiol 45:1178–1182
Hynes RK, Knowles R (1984) Production of nitrous oxide by Nitrosomonas europaea: effects of acetylene, pH and oxygen. Can J Microbiol 30:1397–1404
Koops HP, Harms H (1985) Deoxyribonucleic acid homologies among 96 strains of ammonia-oxidizing bacteria. Arch Microbiol 141:214–218
L'Air liquide (ed) (1976) Gas encyclopaedia. Elsevier, Amsterdam
Lipschultz F, Zafiriou OC, Wofsy SC, McElroy MB, Valois FW, Watson SW (1981) Production of NO and N2O by soil nitrifying bacteria. Nature 294:641–643
Logan JA (1983) Nitrogen oxides in the troposphere: global and regional budgets. J Geophys Res 88:10785–10807
Meincke M, Krieg E, Bock E (1989) Nitrosovibrio spp., the dominant ammonia-oxidizing bacteria in building sandstone. Appl Environ Microbiol 55:2108–2110
Miller DJ, Wood PM (1983) The soluble cytochrome oxidase of Nitrosomonas europaea. J Gen Microbiol 129:1645–1650
Nicholas DJD, Jones OTG (1960) Oxidation of hydroxylamine in cell-free extracts of Nitrosomonas europaea. Nature 185:512–514
Poth M (1986) Dinitrogen production from nitrite by a Nitrosomonas isolate. Appl Environ Microbiol 52:957–959
Poth M, Focht DD (1985) 15N kinetic analysis of N2O production by Nitrosomonas europaea: an examination of nitrifier denitrification. Appl Environ Microbiol 49:1134–1141
Remde A, Slemr F, Conrad R (1989) Microbial production and uptake of nitric oxide in soil. FEMS Microbiol Ecol 62:221–230
Ritchie GAF, Nicholas DJD (1972) identification of the source of nitrous oxide produced by oxidative and reductive processes in Nitrosomonas europaea. Biochem J 126:1181–1191
Schlichting E, Blume HO (1966) Bodenkundliches Praktikum. Paul Parey, Hamburg
Van Cleemput O, Beart L, (1976) Theoretical considerations on nitrite self-decomposition reactions in soils. Soil Sci Soc Am J 40:322–324
Van Cleemput O, Baert L (1984) Nitrite: a key compound in N loss processes under acid conditions. Plant Soil 76:233–241
Ward BB, Zafiriou OC (1988) Nitrification and nitric oxide in the oxygen minimum of the easter tropical North Pacific. Deep-Sea Res 35:1127–1142
Zafiriou OC, Hanley Q, Snyder G (1989) Nitric oxide and nitrous oxide production and cycling during dissimilatory nitrite reduction by Pseudomonas perfectomarina. J Biol Chem 264: 5694–5699
Zumft WG, Viebrock A, Körner H (1988) Biochemical and physiological aspects of denitrification. In: Cole JA, Ferguson S (eds) The nitrogen and sulphur cycles. Cambridge University Press, Cambridge, pp 245–279
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Remde, A., Conrad, R. Production of nitric oxide in Nitrosomonas europaea by reduction of nitrite. Arch. Microbiol. 154, 187–191 (1990). https://doi.org/10.1007/BF00423331
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DOI: https://doi.org/10.1007/BF00423331