Summary
GLP-1(7–36)amide is an intestinal posttranslational proglucagon product released mainly after carbohydrate ingestion, the glucose dependent insulinotropic and antidiabetogenic actions of which have been documented. In this work, by exploring whether GLP-1(7–36)amide has any effect on the glucose metabolism of the muscle, we have observed that this peptide, at physiological concentrations, exerts in this tissue an increment of the d-[U-14C] glucose incorporated into glycogen, which is accompanied by an increase in the glycogen synthase a activity; also, it stimulates both glucose oxidation and lactate formation. These data indicate that the skeletal muscle is one of the target tissues for GLP-1(7–36)amide, where its insulin-like effect explains, at least in part, its plasma glucose lowering action; thus, GLP-1(7–36)amide may well be implicated in the physiological control of glucose homeostasis after meals, not only by acting as an incretin, but also by directly promoting glucose disposal.
Article PDF
Similar content being viewed by others
Abbreviations
- KRB:
-
Krebs-Ringer bicarbonate buffer, pH 7.4
- EDTA:
-
ethylenedinitrilo tetraacetic acid, disodium salt dihydrate
- BSA:
-
bovine serum albumin
References
Orskov C (1992) Glucagon-like peptide-1, a new hormone of the entero-insular axis. Diabetologia 35: 701–711
Gutniak M, Orskov C, Holst JJ, Ahrén B, Efendic S (1992) Antidiabetogenic effects of glucagon-like peptide-1(7–36)amide in normal subjects and patients with diabetes mellitus. N Engl J Med 326: 1316–1322
Marchand-Brustel YM, Freychet P (1981) Regulation of glycogen synthase activity in the isolated mouse soleus muscle. Effect of insulin, epinephrine, glucose and anti-insulin receptor antibodies. Biochim Biophys Acta 677: 13–22
Cuendet GS, Ernest G, Loten BJ, Renold A (1976) Decreased basal, noninsulin-stimulated glucose uptake and metabolism by skeletal soleus muscle isolated from obesehyperglycemic (ob/ob) mice. J Clin Invest 58: 1078–1088
Cámara J, Galera C, Valverde I, Malaisse WJ (1991) Relationship between d-glucose oxidation and glycolysis in tumoral pancreatic islet cells with either rapid or decreased cell growth. Diabetes 17: 67–71
Hue L, Bontemps F, Hers HG (1975) The effect of glucose and of potassium ions on the interconversion of glycogen phosphorylase and of glycogen synthetase in isolated rat liver preparation. Biochem J 152: 105–114
Fleig WE, Noether-Fleig G, Fussgaenger R, Ditschuneit H (1984) Modulation by a sulfonylurea of insulin-dependent glycogenesis, but not of insulin binding, in cultured rat hepatocytes. Diabetes 33: 285–290
Wheeler MB, Lu M, Dillon JS, Leng XH, Chen C, Boyd AE III (1993) Functional expression of the rat glucagonlike peptide-1 receptor, evidence for coupling to both adenylyl cyclase and phospholipase-C. Endocrinology 133: 57–62
Delgado E, Alcántara A, Trapote MA, Valverde I, Villanueva-Peñacarrillo ML (1993) Identification and characterization of GLP-1(7–36)amide receptors in rat skeletal muscle. Diabetologia 36: A39 (Abstract)
Thorens B (1992) Expression cloning of the pancreatic beta-cell receptor for the gluco-incretion hormone glucagon-like peptide 1. Proc Natl Acad Sci USA 89: 8641–8645
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Villanueva-Peñacarrillo, M.L., Alcántara, A.I., Clemente, F. et al. Potent glycogenic effect of GLP-1(7–36)amide in rat skeletal muscle. Diabetologia 37, 1163–1166 (1994). https://doi.org/10.1007/BF00418382
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00418382