Abstract
A mutant in Saccharomyces cerevisiae required one hundred times more K+ than wild type for the same half maximal growth rate. Mutant cells and wild type cells grown at millimolar K+ did not show significant differences in Rb+ transport. In the mutant, a rapid K+ loss induced by azide or incubation (4 h) in K+-free medium decreased the Rb+ transport K m by one half; in the wild type, those treatments decreased the Rb+ K m twenty and one hundred times, respectively. Mutant and wild type did not show significant differences in Na+ transport and in the Na+ inhibition of Rb+ transport, either in normal-K+ cells or in K+-starved cells. The results suggest that either two systems or one system with two interacting sites mediate K+ transport in S. cerevisiae.
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Abbreviations
- YPD:
-
yeast-peptone-dextrose medium
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Ramos, J., Contreras, P. & Rodríguez-Navarro, A. A potassium transport mutant of Saccharomyces cerevisiae . Arch. Microbiol. 143, 88–93 (1985). https://doi.org/10.1007/BF00414774
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DOI: https://doi.org/10.1007/BF00414774