Abstract
The cell wall teichoic acid structures of 22 staphylococci including 13 type strains were determined. Most of the strains contain a poly(polyolphosphate) teichoic acid with glycerol and/or ribitol as polyol component. The polyolphosphate backbone is partially substituted with various combinations of sugars and/or amino sugars. Most of the substituents occur in a monomeric form but some strains also contain dimers of N-acetylglucosamine as substituents. Staphylococcus hyicus subsp. hyicus NCTC 10350 and S. sciuri DSM 20352 revealed rather complex cell wall teichoic acids. They consist of repeating sequences of phosphate-glycerol-phosphate-N-acetylglucosamine. The amino sugar component is present in this case as a monomer or an oligomer (n≤3). Moreover, the glycerol residues are partially substituted with N-acetylglucosamine. The cell wall teichoic acid of S. auricularis is a poly(N-acetylglucosaminylphosphate) polymer similar to that found in S. caseolyticus ATCC 29750. The cell wall teichoic acid structures for type strains of S. auricularis, S. capitis, S. cohnii, S. haemolyticus, S. hominis, S. hyicus subsp. hyicus, S. sciuri, S. xylosus and S. warneri were determined for the first time in detail. The structures of some of the previously described teichoic acids had to be revised (S. epidermidis, S. simulans, S. aureus phage type 187).
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Abbreviations
- GalN:
-
galactosamine
- GlcN:
-
glucosamine
- Gro:
-
glycerol
- Rit:
-
ribitol
References
Aasen J, Oeding P (1971) Antigenic studies on Staphylococcus epidermidis. Acta Path Microbiol Scand Sect B 79:827–834
Albersheim P, Nevins DJ, English PD, Karr A (1967) A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography. Carbohyd Res 5:340–345
Ames BW (1966) Assay of inorganic phosphate, total phosphate and phosphatases. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol VIII. Academic Press, New York, pp 115–118
Archibald AR, Baddiley J, Button D (1968a) The glycerol teichoic acid of walls of Staphylococcus lactis I3. Biochem J 110:543–557
Archibald AR, Baddiley J, Button D, Heptinstall S, Stafford GH (1968b) Occurrence of polymers containing N-acetylglucosamine 1-phosphate in bacterial walls. Nature (London) 219:855–856
Archibald AR, Baddiley J, Shaukat GA (1968c) The glycerol teichoic acid from walls of Staphylococcus epidermidis I2. Biochem J 110:583–588
Archibald AR, Baddiley J, Heckels JE, Heptinstall S (1971) Further studies on the glycerol teichoic acid of walls of Staphylococcus lactis I3. Biochem J 125:353–355
Archibald AR (1972) The chemistry of staphylococcal cell walls. In: Cohen JO (ed) The staphylococci Wiley-Interscience, New York, pp 75–109
Archibald AR, Stafford GH (1972) A polymer of N-acetylglucosamine 1-phosphate in the wall of Staphylococcus lactis 2102. Biochem J 130:681–690
Baddiley J, Buchanan JG, Hardy FE, Martin RO, RajBhandry UL, Sanderson AR (1961) The structure of the ribitol teichoic acid of S. aureus H. Biochim Biophys Acta 52:406–407
Baddiley J, Buchanan JG, RajBhandary UL, Sanderson AR (1962) Teichoic acids from the walls of Staphylococcus aureus H. 1. Structure of the N-acetylglucosaminylribitol residues. Biochem J 82:439–448
Bergmeyer HU (1974) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim
Davison AL, Baddiley J (1964) Glycerol teichoic acid in walls of Staphylococcus epidermidis. Nature 202:874
Endresen C, Grov A, Oeding P (1974) Immunochemical studies of three cell wall polysaccharides from animal coaglulase-positive staphylococci. Acta Path Microbiol Scand Sect B 82:382–386
Enghofer E, Kress H (1979) A evaluation of the Morgan-Elson assay for 2-amino-2-deoxy sugars. Carbohyd Res 70:233–238
Fiedler F, Schäffler MJ, Stackebrandt E (1981) Biochemical and nucleic acid hybridization studies on Brevibacterium linens and related strains. Arch Microbiol 129:85–93
Ghuysen JM, Strominger JL (1963) Structure of the cell wall of Staphylococcus aureus, strain Copenhagen. I. Preparation of fragments by enzymatic hydrolysis. Biochemistry 2:1110–1119
Hanes CS, Isherwood FA (1949) Separation of phosphoric esters on the filter paper chromatogram. Nature 164:1107–1112
Hajek V (1976) Staphylococcus intermedius, a new species from animals. Int J Syst Bacteriol 26:401–408
Hoffmann J, Lindberg B, Svensson S (1972) Determination of the anomeric configuration of sugar residues in acetylated oligo- and polysaccharides by oxidation with chromium trioxide in acetic acid. Acta Chem Scand 26:661–666
Jeanloz RW, Trémège M (1956) Colour reaction of N-acetylamino sugar derivatives with p-dimethylaminobenzaldehyde. Fed Proc 15:282
Karakawa WW, Kane JA (1971) Immunochemical analysis of a galactosamine-rich teichoic acid of Staphylococcus aureus, phage type 187. J Immunol 106:900–906
Kidby DK, Davidson DJ (1973) A convenient ferricyanide estimation of reducing sugars in the nanomol range. Anal Biochem 55:321–325
Kloos WE, Schleifer KH (1975) Isolation and characterization of staphylococci from human skin. II. Description of four new species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus hominis, and Staphylococcus simulans. Int J Syst Bacteriol 25:62–79
Kloos WE, Schleifer KH (1983) Description of Staphylococcus auricularis sp nov: An inhabitant of the human external ear. Int J Syst Bacteriol 33:9–14
Morse SJ (1963) Isolation and immunological properties of a group antigen of Staphylococcus albus. J Exp Med 117:19–26
Oeding P, Myklested B, Davison AL (1967) Serological investigation on teichoic acids from the wall of S. epidermidis and Micrococcus. Acta Patho Microbiol Scand 69:458–464
Oeding P (1978) Oenus Staphylococcus. In: Bergan T, Norris JR (eds) Methods in microbiology, vol 12. Academic Press, London, pp 127–176
Osland A, Grov A, Oeding P (1979) Immunochemical analysis of the teichoic acid from Staphylococcus hyicus. Acta Path Microbiol Scand Sect B 87:165–169
Osland A, Grov A, Oeding P (1980) Immunochemical analysis of the teichoic acid from Staphylococcus simulans. Acta Path Microbiol Scand Sect B 88:121–123
Schleifer KH, Kandler O (1967) Zur chemischen Zusammensetzung der Zellwände der Streptokokken. I. Zur Aminosäuresequenz des Mureins von Str. thermophilus and Str. faecalis. Arch Mikrobiol 57:335–365
Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
Schleifer KH, Kloos WE (1975) Isolation and characterization of staphylococci from human skin. I. Amended descriptions of Staphylococcus epidermidis and Staphylococcus saprophyticus and description of three new species: Staphylococcus cohnii, Staphylococcus haemolyticus, and Staphylococcus xylosus. Int J Syst Bacteriol 25:50–61
Schleifer KH, Kocur M (1973) Classification of staphylococci based on chemical and biochemical properties. Arch Mikrobiol 93:65–85
Schleifer KH, Schumacher-Perdreau F, Götz F, Popp B (1976) Chemical and biochemical studies for the differentiation of coagulase-positive staphylococci. Arch Microbiol 110:263–270
Shapira J (1969) Identification of sugars as their trifluoracetyl polyol derivatives. Nature 22:792–793
Trevelyan WE, Procter DD, Harrison JS (1950) Detection of sugars on paperchromatograms. Nature 116:444–445
Weissmann B, Rowin G, Marshall J, Friedeici D (1967) Mammalian α-acetylglucosaminidase. Enzymic properties, tissue distribution, and intracellular localization. Biochemistry 6:207–214
Yamaguchi H, Mukumoto T (1977) GLC analysis of the hydroxy and oxo compounds produced by the Smith degradation of reducing di-and trisaccharides. J Biochem 82:1673–1680
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Endl, J., Seidl, H.P., Fiedler, F. et al. Chemical composition and structure of cell wall teichoic acids of staphylococci. Arch Microbiol 135, 215–223 (1983). https://doi.org/10.1007/BF00414483
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DOI: https://doi.org/10.1007/BF00414483