Abstract
Chromatophores from Rhodopseudomonas capsulata grown photoheterotrophically with malate as both reductant and carbon source contain at least six iron sulphur centres. These are a High Potential Iron Sulphur Protein (HiPIP, probably centre S-3 of the succinic-dehydrogenase), a Rieske centre (centered at g=1.90) and four ferredoxins centered at g=1.94. Previous studies on mutants (Zannoni and Ingledew (1983) FEMS Lett 17, 331–334) have shown that two of these ferredoxins may be assigned to the NADH-dehydrogenase and two to the succinic-dehydrogenase. In this paper we report a complete analysis of the wild-type strain and attempt a functional characterization of the iron sulphur centres by studying their oxidation-reduction behaviour during various steady and inhibited states in the light or the dark. We conclude that the Rieske centre is involved in both respiratory and photosynthetic electron transport and is located on the oxygen side of the antimycin A-block. Conversely the HiPIP centre seems to be located on the succinate-cyt. b/c 1 oxido-reductase pathway although apparently in equilibrium with photosynthetic electron transport. We confirm from steady state studies in the wild type our previous analysis of the g=1.94 ferredoxins using mutant strains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baccarini-Melandri A, Zannoni D, Melandri BA (1973) Energy transduction in photosynthetic bacteria. VI. Respiratory sites of energy conservation in membranes from dark-grown cells of Rhodopseudomonas capsulata. Biochem Biophys Acta 314:298–311
Bevington PR (1969) Data reduction and error analysis for the physical sciences. McGraw Hill, New York
Carithers RP, Yoch DC, Arnon DI (1977) Isolation and characterization of bound iron sulphur proteins from bacterial photosynthetic membranes. II. Succinate dehydrogenase from Rhodospirillum rubrum chromatophores. J Biol Chem 252:7461–7467
Cramer WA, Crofts AR (1982) Electron and proton transport. In: Govindjee (ed) Photosynthesis, vol 1. Academic Press, pp 387–467
Dutton PL, Leigh JS (1973) Electron spin resonance characterization of Chromatium D hemes, non-heme irons and the components involved in primary photochemistry. Biochim Biophys Acta 314:178–190
Dutton PL, Wilson DF, Lee CP (1970) Oxidation-reduction potentials of cytochromes in mitochondria. Biochemistry 9: 5077–5082
Evans MCW, Lord AV, Reeves SG (1974) The detection and characterization by electron paramagnetic resonance spectroscopy of iron-sulphur proteins and other electron transport components in chromatophores from the purple bacterium Chromatium. Biochem J 138:177–183
La Monica RF, Marrs BL (1976) The branched respiratory system of photosynthetically grown Rhodopseudomonas capsulata. Biochim Biophys Acta 423:431–439
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
Malkin R, Chain RK, Kraichoke S, Knaff DB (1981) Studies on the function of the membrane bound iron-sulphur centres of the photosynthetic bacterium Chromatium vinosum. Biochim Biophys Acta 637:88–95
Marrs BL, Stahl CL, Lien S, Gest H (1972) Biochemical physiology of a respiratory deficient mutant of the photosynthetic bacterium Rhodopseudomonas capsulata. Proc Natl Acad Sci USA 69: 916–920
Nelson BD, Norling B, Persson B, Ernster L (1971) Effect of thenoyltri-fluoroacetone on the interaction of succinate dehydrogenase and cytochrome b in ubiquinone-depleted submitochondrial particles. Biochim Biophys Res Comm 44:1312–1320
Ohnishi T (1976) Thermodynamic and EPR characteristics of a HiPIP-type iron-sulphur centre in the succinic dehydrogenase of the respiratory chain. J Biol Chem 251:2105–2109
Prince RC, Ingledew JW (1977) Thermodynamic resolution of the iron-sulphur centres of the succinic-dehydrogenase of Rhodopseudomonas sphaeroides. Arch Biochem Biophys 178:303–307
Prince rC, Lindsay JG, Dutton PL (1975) The Rieske iron-sulphur centre in mitochondrial and photosynthetic systems: Em/pH relationships. FEBS Lett 51:108–111
Zannoni D (1982) ATP-synthesis coupled to light-dependent non-cyclic electron flow in chromatophores of Rhodopseudomonas capsulata. Biochim Biophys Acta 680:1–7
Zannoni D, Ingledew JW (1983) Rhodopseudomonas capsulata respiratory dehydrogenase mutants: an electron paramagnetic responance study. FEMS Lett 17:331–334
Zannoni D, Baccarini-Melandri A, Melandri BA, Evans EH, Prince RC, Crofts AR (1974) Energy transduction in photosynthetic bacteria. The nature of cytochrome c oxidase in the respiratory chain of Rhodopseudomonas capsulata. FEBS Lett 48:152–155
Zannoni D, Melandri BA, Baccarini-Melandri A (1976) Energy transduction in photosynthetic bacteria. X. Composition and function of the branched oxidase system in wild type and respiratory deficient mutants of Rhodopseudomonas capsulata. Biochim Biophys Acta 423:413–430
Zannoni D, Jasper P, Marrs BL (1978) Light-induced oxygen uptake as a probe of electron transport between respiratory and photosynthetic components in membranes of Rhodopseudomonas capsulata. Archiv Biochem Biophys 191:625–631
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zannoni, D., Ingledew, W.J. A functional characterization of the membrane bound iron sulphur centres of Rhodopseudomonas capsulata . Arch Microbiol 135, 176–181 (1983). https://doi.org/10.1007/BF00414475
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00414475