Summary
It is well known that retinoids are effective in the treatment of various dermatological disorders. It has been reported that retinoids have inhibitory effects on the generation of superoxide (O -2 ) by stimulated polymorphonuclear leukocytes (PMNs). In the present study, we investigated the effects of retinoids on the generation of O -2 and other reactive oxygen species (ROS), including OH•, H2O2 and chemiluminescence, by zymosan-stimulated PMNs and in the xanthinexanthine oxidase system, because these potent ROS may play an important role in PMN-mediated skin inflammation. It was found that some retinoids had anti-oxidant effects in the PMN system; however, apart from the effect of tretinoin and Ro 10-1670 on OH• generation, none of the retinoids studied inhibited ROS generation in the xanthine-xanthine oxidase system. On the basis of these results, we discuss a possible mechanism connected with the role of ROS by which retinoids have favourable effects on several inflammatory skin disorders.
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References
Allen RC, Loose LD (1976) Phagocytic activation of a luminol-dependent chemiluminescence in rat alveolar and peritoneal macrophages. Biochem Biophys Res Commun 69:245–252
Baden HP, Buxman MM, Weinstein GD, Yoder FW (1982) Treatment of ichthyosis with isotretinoin. J Am Acad Dermatol 6:716–720
Camisa C, Eisenstat B, Ragaz A, Weissmann G (1982) The effects of retinoids on neutrophil functions in vitro. J Am Acad Dermatol 6:620–629
Caudy AL, Rouchouse B, Boucheron S, Leptit JC (1983) Treatment of cutaneous T cell lymphoma with etretinate. Br J Dermatol 109:49–56
Cheeseman KH (1984) General mechanisms of toxic liver injury with special reference to free radical reactions. Adv Inflam Res 6:179–188
Cohen PP (1957) Suspending media for normal tissues. In: Umbreit WW, Burris RH, Stauffer JF (eds) Monocytometric technique and tissue metabolism, 3rd edn. Burgess Publishing, Minneapolis, pp 149–150
Deluca LM, Sakak W, Adamo S, Bhat PV, Akalovsky J, Silverman-Jones CS, Maestri N (1980) Retinoid metabolism and mode of action. Environ Health Perspect 35:147
Dicken CH, Bauer EA, Hazen PG, Krueger GG, Marks JG Jr, McGuire JS, Schachner LA (1982) Treatment of Darie's disease. J Am Acad Dermatol 6:721–726
Dicken CH, Rochester MN (1984) Retinoids: A review. J Am Acad Dermatol 11:541–552
Fuchs E, Green H (1981) Regulation of terminal differentiation of cultured human keratinocytes by vitamin A. Cell 25:617–625
Goldsmith LA, Weinrich AE, Shupack J (1982) Pityriasis rubra pilaris response to 13-cis retinoic acid (isotretinoin). J Am Acad Dermatol 6:710–715
Haydey RP, Reed ML, Dzubow LM, Shupack JL (1980) Treatment of keratoacanthomas with oral 13-cis retinoic acid. N Engl J Med 303:560–562
Jetten AM, Jetten MER, Shapiro SS, Poon JP (1979) Characterization of the action of retinoids of mouse fibroblast cell lines. Exp Cell Res 119:283–289
Johnston RB Jr, Lehmeyer JE (1976) Elaboration of toxic oxygen by-products by neutrophils in a model of immune complex disease. J Clin Invest 57:836–841
Kessler JF, Meyskens FL Jr, Levine N, Lynch PJ, Jones SE (1983) Treatment of cutaneous T cell lymphoma (mycosis fungoides) with 13-cis-retinoic acid. Lancet 1:1345–1347
Miyachi Y, Niwa Y (1982) Effects of potassium iodide, colchicine, and dapsone on the generation of polymorphonuclear leukocyte-derived oxygen intermediates. Br J Dermatol 107:209–214
Miyachi Y, Niwa Y (1983) Effects of psoriatic sera on the generation of oxygen intermediates by normal polymorphonuclear leukocytes. Arch Dermatol Res 275: 23–26
Miyachi Y, Yanase K, Imamura S, Niwa Y (1982) Increased hydroxyl radical generation by normal polymorphonuclear leukocytes incubated in sera from patients with leukocytoclastic vasculitis. Arch Dermatol Res 274:65–71
Miyachi Y, Horio T, Imamura S (1983) Sunburn cell formation is prevented by scavenging oxygen intermediates. Clin Exp Dermatol 8:305–310
Miyachi Y, Uchida K, Komura J, Asada Y, Niwa Y (1985) Autooxidative damage in cement dermatitis. Arch Dermatol Res 277:288–292
Niwa Y, Sakane T, Miyachi Y (1984) Dissociation of inhibitory effects of dapsone on the generation of oxygen intermediates in comparison with that of colchicine and various scavengers. Biochem Pharmacol 33:2355–2360
Orfanos CE, Bauer E (1983) Evidence for anti-inflammatory activities of oral synthetic retinoids: Experimental findings and clinical experience. Br J Dermatol 109 (suppl 25):55–60
Organos CE, Landes E, Bloch PH (1978) Traitment du psoriasis pustuleux par un nouveau retinoide aromatique (Ro 10-9359) à propos de 9 cas generalises et 8 cas localises. Ann Dermatol Venereol 105:807–811
Peck RP, Olsen TG, Brutkus D, Pandya M, Arnaud-Battandier J, Yoder F, Levis WR (1979) Treatment of basal cell carcinoma with 13-cis retinoic acid. Proc Am Assoc Cancer Res Am Soc Clin Oncol 20:56
Sedwick JB, Bergstresser PR, Hurd ER (1981) Increased superoxide generation by normal granulocytes incubated in sera from patients with psoriasis. J Invest Dermatol 76:158–163
Skosey JL, Chow DC, Damgaard E, Sorensen LB (1973) Effects of cytochalasin B on the response of human polymorphonuclear leukocytes to zymosan. J Cell Biol 57:237–240
Witz G, Goldstein BD, Amruso M, Stone DS, Troll W (1980) Retinoid inhibition of superoxide anion radical production by human polymorphonuclear leukocytes stimulated with tumor promoters. Biochem Biophys Res Commun 97:883–888
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Yoshioka, A., Miyachi, Y., Imamura, S. et al. Anti-oxidant effects of retinoids on inflammatory skin diseases. Arch Dermatol Res 278, 177–183 (1986). https://doi.org/10.1007/BF00412920
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DOI: https://doi.org/10.1007/BF00412920