Abstract
Ethanol grown Acetobacter aceti differed from acetate grown. In ethanol grown cells, acetate uptake, caused by the oxidation of acetate, was completely inhibited by ethanol, in acetate grown cells only to 20%. This was correlated with a 65-fold higher specific activity of the membrane bound NAD(P)-independent alcohol dehydrogenase in ethanol grown than in acetate grown cells. In comparison with ethanol grown cells, acetate grown cells showed a 3-fold higher acetate respiration rate and 3-fold higher specific activities of some tricarboxylic acid cycle enzymes tested. Both adaptations were due to induction by the homologous and not to repression by the heterologous growth substrate.
A. aceti showed a membrane bound NAD(P)-independent malate dehydrogenase and no activity of a soluble NAD(P)-dependent one, as was known before from A. xylinum. A hypothesis was proposed explaining the observed inhibition of malate dehydrogenase and of functioning of the tricarboxylic acid cycle in the presence of ethanol or butanol or glucose by a competition of two electron currents for a common link in the convergent electron transport chains. The electrons coming from the quinoproteins, alcohol dehydrogenase and glucose dehydrogenase on the one side and those coming from the flavoproteins, malate dehydrogenase and succinate dehydrogenase via ubiquinonecytochrome c reductase on the other side are meeting at cytochrome c. Here the quinoproteins may be favoured by higher affinity and so inhibit the flavoproteins. Inhibition could be alleviated in the cell free system by increasing the oxygen supply.
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References
Adachi O, Miyagawa E, Shinagawa E, Matsushita K, Ameyama M (1978) Purification and properties of particulate alcohol dehydrogenase from Acetobacter aceti. Agric Biol Chem 42:2331–2340
Adachi O, Matsushita K, Shinagawa E, Ameyama M (1980) Crystallization and properties of NADP-dependent aldehyde dehydrogenase from Gluconobacter melanogenus. Agric Biol Chem 44:155–164
Adachi O, Shinagawa E, Matsushita K, Ameyama M (1982) Crystallization of membrane-bound alcohol dehydrogenase of acetic acid bacteria. Agric Biol Chem 46:2859–2863
Alpes I, Stürzl E, Scherer S, Böger P (1984) Interaction of photosynthetic and respiratory electron transport in blue-green algae: Effect of a cytochrome c-533 specific antibody. Z Naturforsch 39c:623–626
Ameyama M, Adachi O (1982a) Alcohol dehydrogenase from acetic acid bacteria, membrane bound. In: Wood WA (ed) Methods in enzymology, vol 89. Academic Press, Inc., London New York, pp 450–457
Ameyama M, Adachi O (1982b) Aldehyde dehydrogenase from acetic acid bacteria, membrane bound. In: Wood WA (ed) Methods in enzymology, vol 89. Academic Press, Inc., London New York, pp 491–497
Ameyama M, Osada K, Shinagawa E, Matsushita K, Adachi O (1981a) Purification and characterization of aldehyde dehydrogenase of Acetobacter aceti. Agric Biol Chem 45:1889–1890
Ameyama M, Shinagawa E, Matsushita K, Adachi O (1981b) d-Glucose dehydrogenase of Gluconobacter suboxydans: Solubilization, purification and characterization. Agric Biol Chem 45:851–861
Bailey JE, Ollis DF (1977) Biochemical engineering fundamentals. Mc Graw Hill Kogakusha, Tokyo, p 753
Benziman M (1969) Malate dehydrogenase (FAD-linked) from Acetobacter xylinum. In: Lowenstein JM (ed) Methods in enzymology, vol 13. Academic Press, Inc., London New York, pp 129–134
Binder A (1982) Respiration and photosynthesis in energy transducing membranes of cyanobacteria. J Bioenerget Biomembr 14:271–286
Boehringer Mannheim GmbH (1973) Acetate kinase (AK). In: Biochemica information I. Boehringer Mannheim GmbH, p 10
De Ley J, Gillis M, Swings J (1984) Family VI. Acetobacteraceae. In: Buchanan RE, Gibbons NE (eds) Bergey's manual of systematic bacteriology, vol 1. The Williams & Wilkins Co., Baltimore London, pp 267–278
Divies C (1972) Action de l'ethanol sur l'oxidation de l'acetate par Acetobacter rancens. Biochimie 54:1073–1083
Duine JA, Frank J (1981) Quinoproteins: A novel class of dehydrogenases. Trends Biochem Sci 6:278–280
Duine JA, Frank J, Van der Meer R (1982) Dufferent forms of quinoprotein aldose-(glucose-)dehydrogenase in Acinobacter calcoaceticus. Arch Microbiol 131:27–31
Flückiger J, Ettlinger L (1977) Glucose metabolism in Acetobacter aceti. Arch Microbiol 114:183–187
Greenfield S, Claus GW (1972) Nonfunctional tricarboxylic acid cycle and the mechanism of glutamate biosynthesis in Acetobacter suboxydans. J Bacteriol 112:1295–1302
Holms WH, Bennett PM (1971) Regulation of isocitrate dehydrogease activity in Escherichia coli on adaptation to acetate. J Gen Microbiol 65:57–68
Huber J (1982) Beiträge zum Glucose-Stoffwechsel in Acetobacter aceti. Dissertation ETH Zürich, Nr. 7009
Jones ME, Lipmann F (1955) Aceto-CoA-kinase. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 1. Academic Press, Inc., London New York, pp 585–591
Kashiwagura T, Wilson DF, Erecińska M (1984) Oxygen dependence of cellular metabolism: The effect of O2 tension on gluconeogenesis and urea synthesis in isolated rat hepatocytes. J Cell Physiol 120:13–18
Koch M (1980) Untersuchungen zum Kohlenstoff-Metabolismus in Acetobacter aceti. Dissertation ETH Zürich, Nr. 6537
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Muraoka H, Watanabe Y, Ogasawara N, Takahashi H (1982) Purification and properties of coenzyme independent alcohol dehydrogenase from the membrane fraction of Acetobacter aceti. J Ferm Technol 60:41–50
O'Sullivan J, Ettlinger L (1976) Characterization of the acetyl-CoA synthetase of Acetobacter aceti. Biochim Biophys Acta 450:410–417
Reed LJ, Mukherjee BB (1969) α-Ketoglutarate dehydrogenase complex from Escherichia coli. In: Lowenstein J (ed) Methods in enzymology, vol 13. Academic Press, Inc., London New York, pp 55–61
Sandmann G, Malkin R (1984) Light inhibition of respiration is due to a dual function of the cytochrome b6-f complex and the plastocyanin/cytochrome c-553 pool in Aphanocapsa. Arch Biochem Biophys 234:105–111
Scherer S, Stürzl E, Böger P (1982) Interaction of respiratory and photosynthetic electron transport in Anabaena variabilis Kütz. Arch Microbiol 132:333–337
Shimizu H, Miyai K, Matsuhisa H, Iwasaki E, Tomoyeda M (1977) Effect of ethanol on acetate oxidation by Acetobacter aceti. Eur J Appl Microbiol 3:303–311
Stürzl E, Scherer S, Böger P (1984) Interaction of respiratory and photosynthetic electron transport, and evidence for membrane bound pyridine-nucleotide dehydrogenases in Anabaena variabilis. Physiol Plant 60:479–483
Wang CH (1972) Radiorespirometric methods. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 6B. Academic Press, Inc., London New York, pp 185–230
Weitzman PDJ (1969) Citrate synthase from Escherichia coli. In: Lowenstein JM (ed) Methods in enzymology, vol 13. Academic Press, Inc., London New York, pp 22–26
Yamada Y, Aida K, Uemura T (1969) Enzymatic studies on the oxidation of sugar and sugar alcohol. V. Ubiquinone of acetic acid bacteria and its relation to classification of genera Gluconobacter and Acetobacter especially of the so-called intermediate strains. J Gen Appl Microbiol 15:181–196
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Dedicated to Professor Carl Martius on the occasion of his 80th birthday, March 1st 1986
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Jucker, W., Ettlinger, L. The inhibition of acetate oxidation by ethanol in Acetobacter aceti . Arch. Microbiol. 143, 283–289 (1985). https://doi.org/10.1007/BF00411251
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DOI: https://doi.org/10.1007/BF00411251