Abstract
The synthesis of methanol dehydrogenase, formaldehyde dehydrogenase, and formate dehydrogenase by pink pigmented facultative methylotrophs (PPFM) has been studied during growth on C1 and multicarbon substrates. In batch cultures, the methanol dehydrogenase activities were higher during slow growth on non-C1-compounds than during fast growth on methanol. Derepression of this enzyme also occurred at slow growth in methanol-limited chemostat culture. Formaldehyde dehydrogenase and formate dehydrogenase remained largely repressed during growth on multicarbon substrates.
Similar content being viewed by others
References
Anthony C (1982) The biochemistry of methylotrophs. Academic Press, London New York Paris
Babel W, Mothes G (1980) Rolle der Formiat-Dehydrogenase in “Serinweg-Bakterien”. Z Allg Mikrobiol 20:167–175
Eggeling L, Sahm H (1978) Derepression and partial insensitivity to carbon catabolite repression of the methanol dissimilating enzymes in Hansenula polymorpha. Europ J Appl Microbiol Biotechnol 5:197–202
Egli T, Harder W (1984) Growth of methylotrophs on mixed substrates. In: Crawford RL, Hanson RS (eds) Microbial growth on C1 compounds. American Society for Microbiology, Washington, pp 330–337
Harder W, Dijkhuizen L (1982) Strategies of mixed substrates utilization in microorganism. Phil Trans R Soc Lond B 297:459–480
Harder W, Dijkhuizen L (1983) Physiological responses to nutrient limitation. Ann Rev Microbiol 37:1–23
Harder W, Atwood M, Quayle JR (1973) Methanol assimilation by Hyphomicrobium sp. J Gen Microbiol 78:155–163
Large PJ (1983) Methylotrophy and methanogenesis. Chapter 3, Physiology and biochemistry of methylotrophe bacteria. Van Nostrand Reinhold Co., Ltd, Wokingham, pp 25–56
Large PJ, Quayle JR (1963) Microbial growth on C1 compounds. 5. Enzyme activities in extracts of Pseudomonas AM1. Biochem J 87:386–396
Levering PR, Dijken JP van, Veenhuis M, Harder W (1981) Arthrobacter P1, a fast growing versatile methylotroph with amine oxidase as a key enzyme in the metabolism of methylated amines. Arch Microbiol 129:72–80
Lynch M, Wopat A, O'Connor ML (1980) Characterization of two new facultative methanotrophs. Appl Env Microbiol 40:400–407
Matin A (1981) Regulation of enzyme synthesis as studied in continuous culture In: Calcott PH (ed) Continuous cultures of cells. CRC-Press, Boca Raton, Florida, pp 69–97
McNerney T, O'Connor ML (1980) Regulation of enzymes associated with C-1 metabolism in three facultative methylotrophs. Appl Environ Microbiol 40:370–375
O'Connor ML (1981a) Regulation and genetics in facultative methylotrophic bacteria. In: Dalton H (ed) Microbial growth on C1-compounds. Heyden, London Philadelphia Rheine, pp 294–300
O'Connor ML (1981b) Extension of the model concerning linkage of genes coding for C-1-related functions in Methylobacterium organophilum. Appl Env Microbiol 41:437–441
O'Connor ML, Hanson RS (1977) Enzyme regulation in Methylobacterium organophilum. J Gen Microbiol 101:327–332
Quayle JR (1980) Aspects of the regulation of methylotropic metabolism. FEBS Lett 117 (Suppl): K16-K27
Roitsch T, Stolp H (1985a) Overproduction of methanol dehydrogenase in glucose grown cells of a restricted RuMP type methylotroph. Arch Microbiol 142:34–39
Roitsch T, Stolp H (1985b) Distribution of dissimilatory enzymes in methane and methanol oxidizing bacteria. Arch Microbiol 143:233–236
Weaver CA, Lidstrom ME (1985) Methanol dissimilation in Xanthobacter H4–14: activities, induction and comparison to Pseudomonas AM1 and Paracoccus denitrificans. J Gen Microbiol 131:2183–2197
Wolfrum T, Gruner G, Stolp H (1986) Nucleic acid hybridization of pink pigmented facultative methylotrophs and pseudomonads. Int J Syst Bacteriol 36:24–28
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Roitsch, T., Stolp, H. Synthesis of dissimilatory enzymes of serine type methylotrophs under different growth conditions. Arch. Microbiol. 144, 245–247 (1986). https://doi.org/10.1007/BF00410956
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00410956