Skip to main content
SpringerLink
Log in

Energetics of glucose uptake in Salmonella typhimurium

  • Original Papers
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

We have studied the energetics of glucose uptake in Salmonella typhimurium. Strain PP418 transprots glucose via the phosphoenolpyruvate: glucose phosphotransferase system, while strain PP1705 lacks this system and can only use the galactose permease for glucose uptake. These two strains were cultured anaerobically in glucose-limited chemostats. Both strains produced ethanol and acetate in equimolar amounts but a significant difference was observed in the molar growth yield on glucose (Y Glc). It is suggested that this difference is due to a difference in the energetics of the glucose uptake systems in the two strains.

Assuming an equal Y ATP for both strains, we could calculate that uptake of 1 mole of glucose via the galactose permease consumes the equivalent of 0.5 mole of ATP. With the additional assumption that one proton is transported in symport with one glucose molecule, these results imply a stoichiometry of two protons per ATP hydrolysed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

PTS:

Phosphoenolpyruvate: carbohydrate phosphotransferase system

D :

dilution rate (h-1

DW:

dry weight

GalP:

galactose permease

EtOH:

ethanol

HAc:

acetate

Lact:

lactate

Suc:

succinate

HFo:

formate

Glc:

Glucose

Y Glc, Y ATP :

yield of cells per glucose or ATP

q :

specific production rate

References

  • Bauchop T, Elsden SR (1960) The growth of microorganisms in relation to their energy supply. J Gen Microbiol 23:457–469

    Google Scholar 

  • Evans CTG, Herbert D, Tempest DW (1970) The continuous cultivation of micro-organisms. 2. Construction of a chemostat. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 2. Academic Press, New York, pp 277–327

    Google Scholar 

  • Gottschalk G (1979) Bacterial fermentations. In: Starr MP (ed) Bacterial metabolism Springer, Berlin Heidelberg New York, pp 167–224

    Google Scholar 

  • Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 5B. Academic Press New York, pp 209–344

    Google Scholar 

  • Hernandez E, Johnson MJ (1967) Anaerobic growth yields of Aerobacter cloacae and Escherichia coli. J Bacteriol 94:991–995

    Google Scholar 

  • Muir M, Williams L, Ferenci T (1985) Influence of transport energetization on the growth yield of Escherichia coli. J Bacteriol 163:1237–1242

    Google Scholar 

  • Nagelkerke F, Postma PW (1978) 2-Deoxygalactose, a specific substrate of the Salmonella typhimurium galactose permease: its use for the isolation of galP mutants. J Bacteriol 133:607–613

    Google Scholar 

  • Perlin SP, San Francisco MJD, Slayman CW, Rosen BP (1986) H+/ATP stoichiometry of proton pumps from Neurospora crassa and Escherichia coli. Arch Biochem Biophys 248:53–61

    Google Scholar 

  • Postma PW (1977) Galactose transport in Salmonella typhimurium. J Bacteriol 129:630–639

    Google Scholar 

  • Postma PW, Lengeler JW (1985) Phosphoenolpyruvate: carbohydrate phosphotransferase system of bacteria. Microbiol Rev 49:232–269

    Google Scholar 

  • Saier Jr, MH, Bromberg FG, Roseman S (1973) Characterization of constitutive galactose permease mutants in Salmonella typhimurium. J Bacteriol 113:512–514

    Google Scholar 

  • Teixeira de Mattos MJ (1984) The metabolic response of Klebsiella aerogenes to anaerobic nutrient-limited environments. A chemostat study. PhD Thesis, Elinkwijk, Utrecht

    Google Scholar 

  • Teixeira de Mattos MJ, Tempest DW (1983) Metabolic and energetic aspects of the growth of Klebsiella aerogenes NCTC418 on glucose in anaerobic chemostat culture. Arch Microbiol 134:80–85

    Google Scholar 

  • Tempest DW, Neijssel OM (1984) The status of Y ATP and maintenance energy as biologically interpretable phenomena. Ann Rev Microbiol 38:459–486

    Google Scholar 

  • Thienen GM van, Postma PW, Dam K van (1979) Proton movements coupled to sugar transport via the galactose transport system in Salmonella typhimurium. Eur J Biochem 73:521–527

    Google Scholar 

  • West I, Mitchell P (1973) Stoichiometry of lactose-proton symport across the plasma membrane of Escherichia coli. Biochem J 132:587–592

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Biochemistry, University of Amsterdam, P.O. Box 20151, NL-1000 HD, Amsterdam, The Netherlands

    M. Driessen, P. W. Postma & K. van Dam

Authors
  1. M. Driessen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. W. Postma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. van Dam
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Driessen, M., Postma, P.W. & van Dam, K. Energetics of glucose uptake in Salmonella typhimurium . Arch. Microbiol. 146, 358–361 (1987). https://doi.org/10.1007/BF00410936

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00410936

Key words

Search

Navigation