Abstract
Acetobacter aceti NCIB 8554 grows on a minimal medium with ethanol but not with glucose as carbon and energy source. Addition of glucose to a wild type culture on ethanol has no influence on growth of the organism. Growth of a glucose sensitive mutant A5 is inhibited by the addition of glucose until all glucose has disappeared from the medium. In order to determine the routes by which glucose is metabolised in wild type and mutant, radiorespirometric, enzymatic, and uptake experiments have been performed. For the radiorespirometric experiments of the “continuous substrate feeding” type an apparatus has been constructed.
Of the glucose entering the cells about 30% is excreted as gluconate and 6% metabolised with liberation of C-1 as CO2. The rest is accumulated intracellularly. No differences were found between wild type and mutant.
Under different growth conditions and with different enzymatic assay methods no pyruvate kinase activity (EC 2.7.1.40) could be detected. This might explain the inability of A. aceti to grow on glucose.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PPO:
-
2,5-diphenyloxazole
- DM-POPOP:
-
2,2′-p-phenylene bis(4-methyl-5-phenyloxazole)
- TCA:
-
trichloroacetic acid
References
Bächi, B., Ettlinger, L.: Influence of glucose on adenine nucleotide levels and energy charge in Acetobacter aceti. Arch. Mikrobiol. 93, 155–164 (1973)
Banauch, D., Brümmer, W., Ebeling, W., Metz, H., Rindfrey, H., Lang, H., Leybold, K., Rick, W.: Eine Glucose-Dehydrogenase für die Glucose-Bestimmung in Körperflüssigkeiten. Z. klin. Chem. klin. Biochem. 13, 101–107 (1975)
Benziman, M., Palgi, A.: Characterization and properties of the pyruvate phosphorylation system of Acetobacter xylinum. J. Bact. 104, 211–218 (1970)
Bergmeyer, H. U., Bernt, E., Schmidt, F., Stork, H.: D-Glucose. In: Methoden der enzymatischen Analyse, Vol. 2 (H. U. Bergmeyer, ed.), pp. 1241–1246, 3rd ed. Weinheim: Verlag Chemie 1974
Brand, K.: Transaldolase. In: Methoden der enzymatischen Analyse, Vol. 1 (H. U. Bergmeyer, ed.), pp. 752–756, 3rd ed. Weinheim: Verlag Chemie 1974
Brendel, K., Meezan, E.: A simple apparatus for the continuous monitoring of 14CO2 production from several small reaction mixtures. Anal. Biochem. 60, 88–101 (1974)
De Ley, J., Frateur, J.: Genus Acetobacter Beijerinck 1898. In: Bergey's manual of determinative bacteriology (R. E. Buchanan, N. E. Gibbons, eds.), pp. 276–278, 8th ed. Baltimore, Williams & Wilkins 1974
Flückiger, J.: Glucose-Stoffwechsel bei Acetobacter aceti. Dissertation, ETH Zürich, Nr. 5814 (1976)
Leisinger, Th.: Untersuchungen zu Systematik und Stoffwechsel der Essigsäurebakterien. Zbl. Bakt. 2. Abt. 119, 329–376 (1965)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin-phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Lutz, R. A., Flückiger, J.: Kinetic determination of glucose with the GEMSAEC (ENI) centrifugal analyzer by the glucose dehydrogenase reaction, and comparison with two commonly used procedures. Clin. Chem. 21, 1372–1377 (1975)
Maeba, P., Sanwal, B. D.: The regulation of pyruvate kinase of Escherichia coli by fructose diphosphate and adenylic acid. J. biol. Chem. 243, 448–449 (1968)
Möllering, H., Bergmeyer, H. U.: D-Gluconat. In: Methoden der enzymatischen Analyse, Vol. 2 (H. U. Bergmeyer, ed.), pp. 1288–1292, 3rd ed. Weinheim: Verlag Chemie 1974
Pon, N. G., Bondar, R. J. L.: A direct spectrophotometric assay for pyruvate kinase. Anal. Biochem. 19, 272–279 (1967)
Sanwal, B. D.: Allosteric controls of amphibolic pathways in bacteria. Bacteriol. Revs. 34, 20–39 (1970)
Wang, C. H.: Radiorespirometric methods. In: Methods in microbiology, Vol. 6B (J. R. Norris, D. W. Ribbons, eds.), pp. 185–230. London-New York: Academic Press 1972
Weber, P.: Der Einfluss von Glucose auf den Aethanolstoffwechsel eines Stammes von Acetobacter aceti. Dissertation, ETH Zürich, Nr. 4794 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Flückiger, J., Ettlinger, L. Glucose metabolism in Acetobacter aceti . Arch. Microbiol. 114, 183–187 (1977). https://doi.org/10.1007/BF00410782
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00410782