Abstract
Enumerations of tartrate-fermenting anaerobic bacteria with l-, d-, and m-tartrate as substrates revealed that l-tartrate fermenters outnumbered d- and m-tartrate fermenters by one to three orders of magnitude in all three anoxic environments studied.
Highest numbers of tartrate-fermenting bacteria were found in freshwater creek sediments, less in polluted marine channels, and lowest numbers in anoxic sewage digestor sludge. Prevailing bacteria were isolated on every tartrate enantiomer. They all degraded tartrates via oxaloacetate.
d- and m-tartrate-fermenting anaerobes were able to ferment l-tartrate as well, and were assigned to the genera Bacteroides, Acetivibrio, and Ilyobacter. l-Tartrate-fermenting anaerobes only utilized this enantiomer, and were characterized in more detail. Fermentation products on tartrate, citrate, pyruvate, and oxaloacetate were acetate, formate, and carbon dioxide. On fructose and glucose, also ethanol was formed. Freshwater isolates were Gram-positive cocci with large slime capsules, and were described as a new species, Ruminococcus pasteurii. Saltwater isolates were Gram-negative short rods, and were also described as a new species, Ilyobacter tartaricus. The guanosine-plus-cytosine content of the DNA was 45.2% and 33.1%, respectively.
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References
American Public Health Association Inc (ed) (1969) Standard methods for the examination of water and wastewater including bottom sediments and sludge. New York, pp 604–609
Barker HA (1936) On the fermentation of some dibasic C4-acids by Aerobacter aerogenes. Proc Acad Sci Amst 39:674
Bergmeyer HU (1974) Methoden der enzymatischen Analyse, 3rd edn. Verlag Chmie, Weinheim
Buchanan RE, Gibbons NE (1974) Bergey's manual of determinative bacteriology, 8th edn. Williams and Wilkins Co, Baltimore
Dagley S, Trudgill PW (1963) The metabolism of tartaric acid by a Pseudomonas. A new pathway. Biochem J 89:22–31
De Vries W, Rietveld-Struijck TRM, Stouthamer AG (1977) ATP formation associated with fumarate and nitrate reduction in growing cultures of Veillonella alcalescens. Antonie van Leeuwenhoek 43:153–167
Hartree EF (1972) Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem 48:422–427
Hurlbert RE, Jakoby WB (1965) Tartaric acid metabolism. I. Subunits of l(+)-tartaric acid dehydrase. J Biol Chem 240:2772–2777
Krampitz LO, Lynen F (1956) Formation of oxaloacetate from d-tartrate. Fed Proc 15:292–293
Krieg NR, Holt JG (1984) Bergey's manual of systematic bacteriology, 9th edn, vol 1. Williams and Wilkins, Baltimore London
Kun E (1956) Enzymatic mechanism of oxidation of tartrate. J Biol Chem 221:223–230
Lang E, Lang H (1972) Spezifische Farbreaktion zum direkten Nachweis der Ameisensäure. Z Analyt Chem 260:8–10
La Rivière HWM (1956) Intermediate products in tartrate decomposition by cell-free extracts of Pseudomonas putida under anaerobic conditions. Biochim Biophys Acta 21:190–191
Mercer WA, Vaughn RH (1951) The characteristics of some thermophilic tartrate-fermenting anaerobes. J Bacteriol 62: 27–37
Nomura M, Sakaguchi K (1955) The decomposition of l-(+)-tartrate by microorganisms. J Gen Appl Microbiol 1:77–98
Pasteur L (1858) Mémoire sur la fermentation de l'acide tartrique. Compt Rend Acad Sci Paris 46:615–618
Pfennig N (1978) Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 28:283–288
Rode H, Giffhorn F (1982) Ferrous-or cobalt ion-dependent d-(-)-tartrate dehydratase of pseudomonads: purification and properties. J Bacteriol 151:1602–1604
Schink B, Pfennig N (1982) Fermentation of trihydroxybenzenes by Pelobacter acidigallici gen. nov. sp. nov., a new strictly anaerobic, non-sporeforming bacterium. Arch Microbiol 133:195–201
Shilo M (1957) The enzymic conversion of the tartaric acids to oxaloacetic acids. J Gen Microbiol 16:472–481
Shilo M, Stanier RY (1957) The utilization of the tartaric acids by pseudomonads. J Gen Microbiol 16:482–490
Stieb M, Schink B (1984) Ilyobacter polytropus gen. nov. sp. nov., a new non-sporing strict anaerobe exhibiting a very versatile fermentation metabolism Arch Microbiol (submitted)
Stouthamer AH (1979) The search for correlation between theoretical and experimental growth yields. In: Quayle JR (ed) International review of biochemistry, microbial biochemistry, vol 21. University Park Press, Baltimore, pp 1–47
Tabachnick J, Vaughn RH (1948) Characteristics of tartratefermenting species of Clostridium. J Bacteriol 56:435–443
Van Niel CB (1944) The culture, general physiology, morphology, and classification of the nonsulfar purple and brown bacteria. Bacteriol Rev 8:1–118
Vaughn RH, Marsh GL, Stadtman TC, Cantino BC (1946) Decomposition of tartrates by the coliform bacteria. J Bacteriol 52:311–325
Widdel F, Pfennig N (1981) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from salive environments. Description of Desulfobacter postgatei gen. nov. sp. nov. Arch Microbiol 129:395–400
Windholz M, Budavari S, Stroumtsos LY, Fertig MN (1976) The Merck Index. Merck & Co Inc, Rahway, New Jersey
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Dedicated to Prof. Dr. H. G. Schlegel on occasion of his 60. birthday
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Schink, B. Fermentation of tartrate enantiomers by anaerobic bacteria, and description of two new species of strict anaerobes, Ruminococcus pasteurii and Ilyobacter tartaricus . Arch. Microbiol. 139, 409–414 (1984). https://doi.org/10.1007/BF00408388
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DOI: https://doi.org/10.1007/BF00408388