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Inactivation of Hill reaction by long-wavelength ultraviolet radiation (UV-A) and its photoreactivation by visible light in the cyanobacterium, Anacystis nidulans

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Abstract

The dose effect curve for the inhibition of p-benzoquinone Hill reaction revealed that the long-wavelength ultraviolet radiation (320–390 nm, UV-A) cannot completely inactivate this reaction in the cyanobacterium, Anacystis nidulans. The inactivated Hill reaction is photoreactivated by visible light. Relative quantum responsivity curve for photoreactivation shows peaks at around 440, 630 nm and a minimum at around 520 nm. The reactivation is completely inhibited by 10 μM of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). It is suggested that most part of the photoreactivation is dependent on photosynthetic process. Possible, simultaneous inactivation and reactivation by long-wavelength ultraviolet radiation is suggested as the possible mechanism for inability to cause complete inactivation by the radiation.

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Abbreviations

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

p-BQ:

p-benzoquinone

TES:

50 mM N-tris (hydroxymethyl)-methyl-2 aminoethane sulfonic acid

References

  • Allen MB, Arnon DI (1955) Studies on nitrogen-fixing blue-green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiol 30:366–372

    Google Scholar 

  • Asato Y (1972) Isolation and characterization of ultraviolet lightsensitive mutants of blue-green alga Anacystis nidulans. J Bacteriol 110:1058–1064

    PubMed  Google Scholar 

  • Brandle JR, Campbell WF, Sisson WB, Caldwell MM (1977) Net photosynthesis, electron transport capacity, and ultrastructure of Pisum sativum L. exposed to ultraviolet-B radiation. Plant Physiol 60:165–169

    Google Scholar 

  • Cheniae GM, Martin IF (1967) Photoreactivation of manganese catalyst in photosynthetic oxygen evolution. Biochem Biophys Res Comm 28:89–95

    PubMed  Google Scholar 

  • Cheniae GM, Martin IF (1971) Photoreactivation of the manganese catalyst of O2 evolution. Biochim Biophys Acta 253:167–181

    PubMed  Google Scholar 

  • Delaney SF, Herdman M, Carr NG (1976) Genetics of blue-green algae. In: Lewis RA (ed) Genetics of algae. University of California Press, Berkley Los Angeles, pp 7–28

    Google Scholar 

  • Dulbecco R (1949) Reactivation of ultraviolet inactivated bacteriophage by visible light. Nature (London) 163:949–950

    Google Scholar 

  • Gerhardt B, Wiessner W (1967) On the light-dependent reactivation of photosynthetic activity by manganese. Biochem Biophys Res Comm 28:958–964

    PubMed  Google Scholar 

  • Halldall P (1967) Ultraviolet action spectra in algology. A review. Photochem Photobiol 6:445–460

    PubMed  Google Scholar 

  • Hirosawa T, Miyachi S (1983) Effects of long-wavelength ultraviolet (UV-A) radiation on the growth of Anacystis nidulans. Plant Sci Lett 28:291–298

    Google Scholar 

  • Jones LW, Kok B (1966) Photoinhibition of chloroplast reaction. I. Kinetics and action spectra. Plant Physiol 41:1037–1043

    Google Scholar 

  • Jones LW, Myers J (1964) Enhancement in the blue-green alga, Anacystis nidulans. Plant Physiol 39:938–946

    Google Scholar 

  • Kelner A (1949) Photoreactivation of UV-irradiated Escherichia coli with special reference to the dose reduction principle and to UV-induced mutation. J Bacteriol 58:511–522

    Google Scholar 

  • Klein RM (1978) Plant and near ultra-violet radiation. Bot Rev 44:1–127

    Google Scholar 

  • Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322

    Google Scholar 

  • Malkin S, Jones LW (1968) Photoinhibition and excitation quenching in photosystem II of photosynthesis, from fluorescence induction measurements. Biochim Biophys Acta 162:297–299

    PubMed  Google Scholar 

  • Mantai KE, Wong J, Bishop NI (1970) Comparison studies on the effects of ultraviolet irradiation on photosynthesis. Biochim Biophys Acta 197:257–266

    PubMed  Google Scholar 

  • Okada M, Kitajima M, Butler WL (1976) Inhibition of photosystem I and photosystem II in chloroplasts by UV radiation. Plant Cell Physiol 17:35–43

    Google Scholar 

  • Saito N, Werbin H (1970) Purification of a blue-green algal DNA-photoreactivating enzyme. An enzyme requiring light as a physical cofactor to perform its catalytic function. Biochemistry 9:2610–2620

    PubMed  Google Scholar 

  • Singh PK (1975) Photoreactivation of UV-irradiated blue-green algae and algal virus LPP-1. Arch Microbiol 103:297–302

    PubMed  Google Scholar 

  • Sisson WB, Caldwell MM (1976) Photosynthesis, dark-respiration, and growth of Rumex patientia L. exposed to ultraviolet irradiance (288 to 315 nanometers) simulating a reduced atmospheric ozone column. Plant Physiol 58:563–568

    Google Scholar 

  • Sisson WB (1981) Photosynthesis, growth, and ultraviolet irradiance absorbance of Cucurbita pepe L. leaves exposed to ultraviolet-B radiation (280–315 nm). Plant Physiol 67:120–124

    Google Scholar 

  • Teramura AH (1980) Effects of ultraviolet-B irradiance on soybean. I. Importance of photosynthetically active radiation in evaluating ultraviolet-B irradiance effects on soybean and wheat growth. Physiol Plant 48:333–339

    Google Scholar 

  • Van Baalen C (1968) The effects of ultraviolet irradiation on a coccoid blue-green alga: survival, photosynthesis, and photoreactivation. Plant Physiol 43:1689–1695

    Google Scholar 

  • Van Baalen C, O'Donnell R (1972) Action spectra for ultraviolet killing and photoreactivation in the blue-green alga Agmenellum quadruplicatum. Photochem Photobiol 15:269–274

    PubMed  Google Scholar 

  • Watanabe M, Furuya M, Miyoshi Y, Inoue Y, Iwahashi I, Matsumoto K (1982) Design and performance of the Okazaki Large Spectrograph for photobiological research. Photochem Photobiol 36:491–498

    Google Scholar 

  • Wu JH, Lewin RA, Werbin H (1967) Photoreactivation of UV-irradiated blue-green algal virus LPP-1. Virology 31:657–664

    Article  PubMed  Google Scholar 

  • Yamashita T, Butler W (1968) Inhibition of chloroplast by UV-irradiation and heat-treatment. Plant Physiol 43:2037–2040

    PubMed  Google Scholar 

  • Yamashita T, Tomita G (1976) Light-reactivation of (Tris-washed)-DPIP-treated chloroplasts: manganese incorporation, chlorophyll fluorescence, action spectrum and oxygen evolution. Plant Cell Physiol 17:571–582

    Google Scholar 

  • Yamashita T (1982) Effect of uncouplers on photoreactivation of Tris (pH 8.8) and 2,6-dichlorophenol indophenol-treated chloroplasts. Plant Cell Physiol 23:833–841

    Google Scholar 

  • Zhevner VD, Shestakov SV (1972) Studies on the ultraviolet-sensitive mutant of blue-green alga Synechocystis squilis Snav. Arch Microbiol 86:349–360

    Google Scholar 

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  1. Institute of Applied Microbiology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan

    Takayasu Hirosawa & Shigetoh Miyachi

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  1. Takayasu Hirosawa
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  2. Shigetoh Miyachi
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Hirosawa, T., Miyachi, S. Inactivation of Hill reaction by long-wavelength ultraviolet radiation (UV-A) and its photoreactivation by visible light in the cyanobacterium, Anacystis nidulans . Arch. Microbiol. 135, 98–102 (1983). https://doi.org/10.1007/BF00408016

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  • DOI: https://doi.org/10.1007/BF00408016

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