Abstract
Considerable changes in pigment composition accur during a period of 10 h when dark-grown cultures of PG1, a ζ-carotenic strain of Scenedesmus obliquus, are illuminated. These changes are consistent with a biosynthetic pathway in which 15-cis-phytoene is converted via 15-cis-phytofluene and 15-cis-ζ-carotene into all-trans-gz-carotene and trans-bicyclic carotenoids.
The findings also support the view that the xanthophylls lutein and zeaxanthin are formed from the corresponding carotenes and are especially important in the development of a normal chloroplast structure.
Similar content being viewed by others
Abbreviations
- TLC:
-
thin-layer chromatography
References
Aung Than, Bramley, P. M., Davies, B. H., Rees, A. F.: Stereochemistry of phytoene. Phytochemistry 11, 3187–3192 (1972)
Britton, G.: Later reactions of carotenoid biosynthesis. Pure Appl. Chem. 47, 223–236 (1976)
Britton, G., Powls, R.: Phytoene, phytofluene and ζ-carotene isomers from a Scenedesmus obliquus mutant. Phytochemistry 16, 1253–1255 (1977)
Britton, G., Powls, R., Schulze, R. M.: The effect of illumination on the pigment composition of the ζ-carotenic mutant, PG1, of Scenedesmus obliquus. Arch. Microbiol. 113, 281–284 (1977a)
Britton, G., Lockley, W. J. S., Powls, R., Goodwin, T. W., Heyes, L. M.: Carotenoid transformations during chloroplast development in Scenedesmus obliquus PG1, demonstrated by deuterium labelling. Nature (London) 268, 81–82 (1977b)
Brown, D. J., Britton, G., Goodwin, T. W.: Carotenoid biosynthesis by a cell-free preparation from a Flavobacterium species. Biochem. Soc. Trans. 3, 741–742 (1975)
Claes, H.: Biosynthese von Carotenoiden bei Chlorella. 4. Die Carotinsynthese einer Chlorella-Mutante bei anaerober Belichtung. Z. Naturforsch. 13B, 222–224 (1958)
Claes, H.: Biosynthese von Carotinoiden bei Chlorella. 5. Die Trennung von Licht und Dunkelreaktionen bei der lichtabhängigen Xanthophyllsynthese von Chlorella. Z. Naturforsch. 14B, 4–7 (1959)
Davies, B. H.: Carotene biosynthesis in fungi. Pure Appl. Chem. 35, 1–28 (1973)
Davies, B. H., Taylor, R. F.: Carotenoid biosynthesis—the early steps. Pure Appl. Chem. 47, 211–221 (1976)
Gregonis, D. E., Rilling, H. C.: The stereochemistry of trans-phytoene synthesis. Some observations on lycopersene as a carotene precursor and a mechanism for the synthesis of cis-and trans-phytoene. Biochemistry 13, 1538–1542 (1974)
Hager, A.: Die reversiblen, lichtabhängigen Xanthophyllumwandlungen im Chloroplasten. Ber. Dtsch. Bot. Ges. 88, 27–44 (1975)
Krinsky, N. I.: Function. In: Carotenoids (O. Isler, ed.), pp. 669–716. Basel: Birkhäuser 1971
Kushwaha, S. C., Kates, M., Porter, J. W.: Enzymatic synthesis of C40 carotenes by cell-free preparation from Halobacterium cutirubrum. Canad. J. Biochem. 54, 816–823 (1976)
Powls, R.: A carotenoid-deficient mutant strain of Scenedesmus obliquus with defective chloroplast lamellae. Proc. 2nd Int. Congr. Photosynth. Res. 3, 2611–2618 (1972)
Powls, R., Britton, G.: A series of mutant strains of Scenedesmus obliquus with abnormal carotenoid compositions. Arch. Microbiol. 113, 275–280 (1977)
Qureshi, A. A., Andrewes, A. G., Qureshi, N., Porter, J. W.: The enzymatic conversion of cis-[14C]phytofluene, trans-[14C]-phytofluene, and trans-ζ-[14C]-carotene to more unsaturated acyclic, monocyclic and dicyclic carotenes by a cell-free preparation of red tomato fruits. Arch. Biochem. Biophys. 162, 93–107 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Powls, R., Britton, G. The roles of isomers of phytoene, phytofluene and ζ-carotene in carotenoid biosynthesis by a mutant strain of Scenedesmus obliquus . Arch. Microbiol. 115, 175–179 (1977). https://doi.org/10.1007/BF00406372
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00406372