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Mutants of Saccharomyces cerevisiae cell division cycle defective in cytokinesis. Biosynthesis of the cell wall and morphology

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Abstract

The four temperature-sensitive mutants of Saccharomyces cerevisiae in the cell division cycle defective in cytokinesis (cdc, 3, 10, 11 and 12), have been analyzed with respect to the biosynthesis of the cell wall polymers. After 3 hours of incubation at the non-permissive temperature (37°C) these strains stop growing. The synthesis of glucan, mannan and chitin (wall polymers) level off in a similar time, but glucan, mannan and chitin synthases remained active for at least 4 hours.

If the mutants are analyzed by transmission and scanning electron microscopy different pictures emerge. Two of the mutants cdc 10 and cdc 12, after 3 hours of incubation at 37°C present apparently normal cytoplasms and cell wall surfaces with multiple elongated buds. The other two mutants, cdc 3 and cdc 11, present a completely disarranged cytoplasmic content and damage at the level of the plasma membrane is evident.

These and other observations, suggest that between the execution points of cdc 3 (0.27) and cdc 10 (0.58), essential processes in the assembly of cell membrane occur.

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References

  • Bacon, J. S. D., Farmer, V. C., Jones, D. and Taylor, I. F. 1969. The glucan components of the cell wall of baker's yeast (Saccharomyces cerevisiae) considered in relation to its ultrastructure. — Biochem. J. 114: 557–567.

    PubMed  Google Scholar 

  • Byers, B. and Goetsch, L. 1976a. A highly ordered ring of membrane-associated filaments in budding yeast. — J. Cell. Biol. 69: 717–721.

    Article  PubMed  Google Scholar 

  • Byers, B. and Goetsch, L. 1976b. Loss of the filamentous ring in cytokinesis-defective mutants of budding yeast. Abstracts of the First International Congress on Cell Biology. — J. Cell. Biol. 70 (2): 35a.

  • Dominguez, A., Elorza, M. V., Villanueva, J. R. and Sentandreu, R. 1980. Regulation of chitin synthase activity in Saccharomyces cerevisiae: Effect of the inhibition of cell division and of synthesis of RNA and protein. — Curr. Microbiol. 3: 263–266.

    Google Scholar 

  • Dominguez, A., Villanueva, J. R. and Sentandreu, R. 1978. Inositol deficiency in Saccharomyces cerivisiae NCYC 86. — Antonie van Leeuwenhoek 44: 25–34.

    PubMed  Google Scholar 

  • Elorza, M. V., Larriba, G., Villanueva, J. R. and Sentandreu, R. 1977. Biosynthesis of the yeast cell wall: selective assays and regulation of some mannosyl transferase activities. — Antonie van Leeuwenhoek 43: 129–142.

    PubMed  Google Scholar 

  • Elorza, M. V., Lostau, C. M., Villanueva, J. R. and Sentandreu, R. 1976. Cell wall synthesis regulation in Saccharomyces cerevisiae. Effect of RNA and protein inhibition. — Biochim. Biophys. Acta 454: 263–272.

    PubMed  Google Scholar 

  • Gascon, S. and Lampen, J. O. 1968. Purification of internal invertase of yeast. — J. Biol. Chem. 243: 1567–1572.

    PubMed  Google Scholar 

  • Hartwell, L. H. 1971. Genetic control of the cell division cycle in yeast IV. Genes controlling bud emergence and cytokinesis. — Exp. Cell Res. 69: 265–276.

    PubMed  Google Scholar 

  • Hartwell, L. H. 1974. Saccharomyces cerevisiae cell cycle. — Bacteriol. Rev. 38: 164–198.

    PubMed  Google Scholar 

  • Hartwell, L. H., Culotti, J. and Reid, B. 1970. Genetic control of the cell division cycle in yeast I. Detection of mutants. — Proc. Nat. Acad. Sci. USA. 66: 352–359.

    PubMed  Google Scholar 

  • Hartwell, L. H., Mortimer, R. K., Culotti, J. and Culotti, M. 1973. Genetic control of the cell division cycle in yeast: V. Genetic analysis of cdc mutants. — Genetics 74: 267–286.

    Google Scholar 

  • Johnson, B. F. and Gibson, E. J. 1966. Autoradiographic analysis of regional cell wall growth of Yeast III. Saccharomyces cerevisiae. — Exp. Cell Res. 41: 580–591.

    PubMed  Google Scholar 

  • Reynolds, E. S. 1963. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. — J. Cell Biol. 17: 208–212.

    Article  PubMed  Google Scholar 

  • Rodriguez, L., Laborda, F. and Sentandreu, R. 1979. Patterns of wall synthesis in Saccharomyces cerevisiae. — Curr. Microbiol. 2: 293–297.

    Google Scholar 

  • Rodriguez, L., Ruiz, T., Villanueva, J. R. and Sentandreu, R. 1979. Yeast invertase: Subcellular distribution and possible relationships between the isoenzymes. — Curr. Microbiol. 1: 41–44.

    Google Scholar 

  • Sentandreu, R., Elorza, M. V. and Villanueva, J. R. 1975. Synthesis of yeast wall glucan. —J. Gen. Microbiol. 90: 13–20.

    PubMed  Google Scholar 

  • Sierra, J. M., Sentandreu, R. and Villanueva, J. R. 1973. Regulation of wall synthesis during Saccharomyces cerevisiae cell cycle. — FEBS Lett. 34: 285–290.

    Article  PubMed  Google Scholar 

  • Spurr, A. R. 1969. A low viscosity epoxy resin embedding medium for electron microscopy. —J. Ultrastruct. Res. 26: 31–43.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Pharmacy, University of Salamanca, Spain

    A. Dominguez, Rosa M. Varona & J. R. Villanueva

  2. Faculty of Biology, University of Salamanca, Spain

    A. Dominguez, Rosa M. Varona & J. R. Villanueva

  3. Institute of Biochemical Microbiology C.S.I.C., University of Salamanca, Spain

    A. Dominguez, Rosa M. Varona & J. R. Villanueva

  4. Department of Microbiology, Faculty of Pharmacy, University of Valencia, Valencia, Spain

    Rafael Sentandreu

Authors
  1. A. Dominguez
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  2. Rosa M. Varona
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  3. J. R. Villanueva
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  4. Rafael Sentandreu
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Additional information

This work was supported in part by a grant from la Comisión de Investigación Científica y Técnica of the Spanish Ministerio de Educación y Ciencia (Project no. 4593-1980).

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Dominguez, A., Varona, R.M., Villanueva, J.R. et al. Mutants of Saccharomyces cerevisiae cell division cycle defective in cytokinesis. Biosynthesis of the cell wall and morphology. Antonie van Leeuwenhoek 48, 145–157 (1982). https://doi.org/10.1007/BF00405199

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