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Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti NZP2213

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Abstract

Phenol-water extraction of Rhizobium loti NZP2213 cells allowed a simultaneous isolation of two structurally different lipopolysaccharides, from the aqueous (LPS-W) and phenol (LPS-P) phase that differed in their sodium doexycholate-PAGE pattern and composition. LPS-W showed a profile indicating an R-type LPS; LPS-P had a cluster of poorly resolved bands in the high-molecular-weight region. LPS-P contained large amounts of 6-deoxy-l-talose (6dTal), and a small amount of 2-O-methyl-6-deoxy-talose (molar ratio ≈30:1), both of which were completely absent in LPS-W. Methylation analysis gave only one major product, 2,4-di-O-methyl-6dTal, indicating that the O-chain is composed of a homopolymer of 1,3-linked 6dTal, having the methylated 6dTal (2-O-me-6dTal) probably localized at the non-reducing end of the O-chain. This homopolymeric O-chain was additionally O-acetylated, as evidenced by GC-MS and by 13C NMR analysis. The lipid A moieties of both LPS-W and LPS-P showed almost identical composition, with six, different 3-OH fatty acids and with two, so far not described, long-chain 4-oxo-fatty acids, all being amide-linked, and with 27-OH-28:0 as the main ester-linked fatty acid. Lipid A was of the lipid ADAG-type, i.e., having a (phosphorylated) 2,3-diamino-2,3-dideoxy-d-glucose-containing lipid A backbone. Lipid ADAG is widespread among species of the α-2 group of Proteobacteria, but has so far not been encountered in any other rhizobial or agrobacterial species.

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Authors and Affiliations

  1. Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-79108, Freiburg i.Br., Germany

    Hubert Mayer

  2. Department of General Microbiology, M. Curie-Skłodowska University, Akademicka 19, PL-20033, Lublin, Poland

    Ryszard Russa, Teresa Urbanik-Sypniewska & Kristina Lindström

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  1. Ryszard Russa
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  2. Teresa Urbanik-Sypniewska
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  3. Kristina Lindström
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  4. Hubert Mayer
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Russa, R., Urbanik-Sypniewska, T., Lindström, K. et al. Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti NZP2213. Arch. Microbiol. 163, 345–351 (1995). https://doi.org/10.1007/BF00404207

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  • DOI: https://doi.org/10.1007/BF00404207

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