Abstract
During somatic embryogenesis in Papaver orientale tissue cultures a permanent starch accumulation and a transient triacylglycerol accumulation were observed. The degradation of the lipids during plantlet development from embryoids was paralleled by an activity increase of the glyoxylate-cycle enzymes malate synthase (EC 4.1.3.2) and isocitrate lyase (EC 4.1.3.1). Fat accumulation and breakdown was interpreted as a reflection of seed formation and germination during normal development.
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Bajracharya, D., Schopfer, P. (1979) Effect of light on the development of glyoxysomal functions in the cotyledons of mustard (Sinapis alba L.) seedlings. Planta 145, 181–186
Bergfeld, R., Hong, Y.N., Kühnl, T., Schopfer, P. (1978) Formation of oleosomes (storage lipid bodies) during embryogenesis and their breakdown during seedling development in cotyledons in Sinapis alba L. Planta 143, 297–307
Clark, B.R., Rubin, R.T., Arthur, R.G. (1968) A new micro method for determination of cholesterol in serum. Anal. Biochem. 24, 27–33
Dixon, G.H., Kornberg, H.L. (1959) Assay methods for the key enzymes of the glyoxylate cycle. Biochem. J. 72, 3 p
Duncombe, W.G. (1963) The colorimetric micro-determination of long-chain fatty acids. Biochem. J. 88, 7–10
Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158
Gerhardt, B., (1978) Microbodies/Peroxisomen pflanzlicher Zellen. Springer, Berlin Heidelberg New York (Cell biology monographs, vol. 5)
Gerlach, E., Deuticke B. (1963) Eine einfache Methode zur Bestimmung von Phosphat in der Papierchromatographie. Biochem. Z. 337, 477–479
Hock, B., Beevers, H. (1966) Development and decline of the glyoxylate-cycle enzymes in water melon seedlings (Citrullus vulgaris Schrad.). Effects of Dactinomycin and Cycloheximide. Z. Pflanzenphysiol. 55, 405–414
Kleinig, H., Kopp, C. (1978) Lipids, lipid turnover and phospholipase D in plant suspension culture cells (Daucus carota) Planta 139, 61–65
Kleinig, H., Hara, S., Schuchmann, R. (1982) Lipid metabolism in plant tissue culture cells. Acetate incorporation; triacylglycerol accumulation. In: Plant tissue culture 1982, pp. 257–258 Fujiwara, ed. Maruzon, Tokyo
Kudielka, R.A., Theimer, R.R. (1983) Loss of competence for glyoxysome formation during somatic embryogenesis in anise (Pimpinella anisum L.) suspension cultures. Plant Cell Rep. 2, 261–264
Kutchan, T.M., Ayabe, S., Krueger, R.J. (1983) Cytodifferentiation and alkaloid accumulation in cultured cells of Papaver bracteatum. Plant Cell Rep. 2, 281–284
Nessler, C.L., (1982) Somatic embryogenesis in the opium poppy, Papaver somniferum. Physiol. Plant. 55, 453–458
Schacterle, R.G., Pollack (1973) A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal. Biochem. 51, 654–655
Schuchmann, R., Wellmann E (1983) Somatic embryogenesis of tissue cultures of Papaver somniferum and Papaver orientale and its relationship to alkaloid and lipid metabolism. Plant Cell Rep. 2, 88–91
Steward, F., Mapes, M., Mears, K. (1958) Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Am. J. Bot. 45, 705–708
Turnham, E., Northcote, D.H. (1984) The incorporation of [1-14C]acetate into lipids during embryogenesis in oil palm tissue cultures. Phytochemistry 23, 35–39
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Hara, S., Falk, H. & Kleinig, H. Starch and triacylglycerol metabolism related to somatic embryogenesis in Papaver orientale tissue cultures. Planta 164, 303–307 (1985). https://doi.org/10.1007/BF00402941
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DOI: https://doi.org/10.1007/BF00402941