Experimentelle untersuchungen zur resorption der endolymphe im innenohr des meerschweinchens

Summary

Substances rendered radioactive (³H-lysine, ¹⁴C-cysteine, ³H-glucose and biological labeled 3H-guinea-pig-protein) were introduced into the cochlear duct of the ginea-pig by means of a micro-pipette and a special manipulating device. Their distribution and resorption in the inner ear was investigated autoradiographically.

Labeled amino acids spread quickly into the whole of the membranous labyrinth, were they were detected. It is interesting to note that the substances with a low molecular weight have passed into the modiolus and reached the internal acoustic canal. It is concluded that the Corti and perilymphatic spaces communicate with the subarchnoidal space via the perineural space of the cochlear nerve and the modiolus.

³H-glucose could not be transformed into a non-labile compound and hence could not be detected autoradiographically.

A high density of ³H-guinea-pig protein was detected in the tectorial membrane (physico-chemical bound) and in Reissner's membrane (resorption). These results confirm experiments with trypan blue and ferritin by the same author.

Zusammenfassung

Radioaktiv markierte Substanzen (³H-Lysin, ¹⁴C-Cystein, ³H-Glucose and biologisch ³H-markiertes Meerschweinchen-Protein) warden mit Hilfe eines Mikromanipulators durch Mikropipetten in den Ductus cochlearis von Meerschweinchen injiziert und ihre Ausbreitung und Resorption im Innenohr autoradiographisch untersucht.

Die markierten Aminosäuren drangen sehr rasch in die Wände des gesamten häutigen Labyrinths ein and waren dort nachzuweisen. Interessant ist, daß die niedermolekularen Substanzen auch in den Modiolus und von da in den inneren Gehörgang gelangten. Es wird gefolgert, daß Corti- und Perilymphräume über die perincuralen Räume des Nervus cochlearis und des Modiolus mit dem äußeren Liquorraum des Gehirns in Verbindung stehen.