Abstract
When dark-grown maize (Zea mays L.) seedlings were exposed to red light (R), Golgi-localized glucan synthetase activity in the mesocotyl began to decrease within 1 h, and fell by approx. 70% in 12 h. The response required at least 10-2 μmol m-2 R and saturated at 100 μmol m-2. Far-red light (FR) alone inhibited glucan synthetase, and FR reversed the inhibition by R back to the level caused by FR alone. Density gradient fractionation indicated that of the major membrane markers only the Golgi-localized glucan-synthetase activity was affected by R. Golgi-localized latent inosine-diphosphatase activity was unaffected. The kinetics of the response, the photon fluence dependence, and the reversibility by FR all correlated with the inhibition by light of elongation of the mesocotyl, indicating that light inhibits growth and glucan synthetase activity by a similar mechanism.
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Abbreviations
- FR:
-
far-red light
- GS:
-
glucan synthetase
- IAA:
-
indole-3-acetic acid
- R:
-
red light
References
Avery, G.S., Jr., Burkholder, P.R., Creighton, H.B. (1937) Polarized growth and cell studies in the first internode and coleoptile of Avena in relation to light and darkness. Bot. Gaz. 89, 1–39
Blaauw, O.H., Blaauw-Jansen, G., van Leeuwen, W.J. (1968) An irreversible red-light-induced growth response in Avena. Planta 82, 87–104
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254
Erickson, R.O., Goddard, D.R. (1951) An analysis of root growth in cellular and biochemical terms. Growth Symp. 10, 89–116
Gorton, H.L., Briggs, W.R. (1980) Phytochrome responses to end-of-day irradiations in light-grown corn grown in the presence and absence of Sandoz 9789. Plant Physiol. 66, 1024–1026
Hall, M.A., Ordin, L. (1968) Auxin-induced control of cellulose synthetase activity in Avena coleoptile sections. In: Biochemistry and physiology of plant growth substances, pp. 659–671, Wightman, F., Setterfield, G., eds. Runge Press, Ottawa
Inge, F.D., Loomis, W.E. (1937) Growth of the first internode of the epicotyl in maize seedlings. Am. J. Bot. 24, 542–547
Iino, M. (1982) Action of red light on indole-3-acetic-acid status and growth in coleoptiles of etiolated maize seedlings. Planta 156, 21–32
Key, J.L., Hanson, J.B., Lund, H.A., Vatter, A.E. (1961) Changes in cytoplasmic particulates accompanying growth in the mesocotyl of Zea mays. Crop Sci. 1, 5–8
Mandoli, D.F., Briggs, W.R. (1981) Phytochrome control of two low-irradiance responses in etiolated oat seedlings. Plant Physiol. 67, 733–739
Mer, C.L. (1966) The inhibition of cell division in the mesocotyl of etiolated oat plants by light of different frequencies. Ann. Bot. 30, 17–23
Ray, P.M. (1973a) Regulation of β-glucan synthetase activity by auxin in pea stem tissue. I. Kinetic aspects. Plant Physiol. 51, 601–608
Ray, P.M. (1973b) Regulation of β-glucan synthetase activity by auxin in pea stem tissue. II. Metabolic requirements. Plant Physiol. 51, 609–614
Ray, P.M. (1977) Auxin-binding sites of maize coleoptiles are localized on membranes of the endoplasmic reticulum. Plant Physiol. 59, 594–599
Ray, P.M. (1979) Maize coleoptile cellular membranes bearing different types of glucan synthetase activity. In: Plant organelles, pp. 135–146, Reid, E., ed. Halstead Press, New York
Ray, P.M., Shininger, T.L., Ray, M.M. (1969) Isolation of β-glucan synthetase particles from plant cells and identification with Golgi membranes. Proc. Natl. Acad. Sci. USA 64, 605–612
Shore, G., Maclachlan, G.S. (1973) Indoleacetic acid stimulates cellulose deposition and selectively enhances certain β-glucan synthetase activities. Biochim. Biophys. Acta 329, 271–282
Silk, W.K., Erickson, R.O. (1979) Kinematics of plant growth. J. Theor. Biol. 76, 481–501
van Overbeek, J. (1936) Growth hormone and mesocotyl growth. Rec. Trav. Bot. Néerl. 33, 333–340
Vanderhoef, L.N., Quail, P.H., Briggs, W.R. (1979) Red light-inhibited mesocotyl elongation in maize seedlings. II. Kinetic and spectral studies. Plant Physiol. 63, 1062–1067
Van Der Woude, W.J., Lembi, C.A., Morrè, D.J., Kindinger, J.I., Ordin, L. (1974) β-Glucan synthetases of plasma membrane and Golgi apparatus from onion stem. Plant Physiol. 54, 333–340
Walton, J.D., Ray, P.M. (1981) Evidence for receptor function of auxin binding sites in maize. Red light inhibition of mesocotyl elongation and auxin binding. Plant Physiol. 68, 1334–1338
Walton, J.D., Ray, P.M. (1982) Auxin controls Golgi-localized glucan synthetase activity in the maize mesocotyl. Planta 156, 309–313
Went, F.W., Thimann, K.V. (1937) Phytohormones. Macmillan, New York
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Walton, J.D., Ray, P.M. Inhibition by light of growth and Golgi-localized glucan synthetase in the maize mesocotyl. Planta 156, 302–308 (1982). https://doi.org/10.1007/BF00397467
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DOI: https://doi.org/10.1007/BF00397467