Abstract
The kinetics of redistribution of endogenous indole-3-acetic acid, cis-abscisic acid and gibberellic acid (+gibberellin A7) in gravistimulated plant organs were followed by immunoassay, during the latent period and the phase of gravitropic curvature. Whereas in maize coleoptile tips, endogenous indole-3-acetic acid accumulated in the lower half of the organ (ratio 65:35, in favour of the lower half) before bending occurred, it was not possible to detect any significant lateral asymmetry of any of the growth regulators assayed in gravitropically reacting root tips of Zea mays L. and Vicia faba L. nor in hypocotyls of Helianthus annus L. Also, no indication was obtained for an exchange of growth regulators between peripheral and central cell layers of the sunflower hypocotyl. Evidence is presented that changes in the properties of the epidermal or subepidermal cell layers located in the lower half of the horizontally placed sunflower hypocotyl are largely responsible for the gravitropic reaction. An alteration in the subcellular compartmentation of IAA may be involved in this process.
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Abbreviations
- ABA:
-
abscisic acid
- GA:
-
gibberellin
- GA3 :
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- TLC:
-
thin-layer chromatography
References
Audus, L.J. (1969) Geotropism. In: The physiology of plant growth and development, pp. 203–342, Wilkins, M.B., ed. McGraw-Hill, London
Atzorn, R., Weiler, E.W. (1983) The immunoassay of gibberellins. I. Radioimmunoassay for the gibberellins A1, A3, A4, A7, A9 and A20. Planta (in press)
Brauner, L. (1966) Versuche zur Analyse der geotropischen Perzeption. X. Über den Einfluß des Schwerefeldes auf die Auxinempfindlichkeit von Helianthus-Hypokotylen. Planta 69, 299–318
Brauner, L., Hager, A. (1958) Versuche zur gravitropischen Perzeption. Planta 51, 115–147
Chanson, A., Pilet, P.E. (1981) Effect of abscisic acid on maize root gravireaction. Plant Sci. Lett. 22, 1–5
Cholodny, N. (1928) Beiträge zur hormonalen Theorie von Tropismen. Planta 6, 118–134
Copeland, E.B. (1900) Studies on the geotropism of stems. Bot. Gaz. (Chicago) 29, 185–196
Dolk, H.E. (1936) Geotropism and the growth substance. Bot. Trav. Bot. Neerl. 33, 509–585
El-Antably, H.M.M., Larsen, P. (1974) Redistribution of endogenous gibberellins in geotropically stimulated roots. Nature (London) 250, 76–77
Feldman, L.J. (1981) Root cap inhibitor formation in isolated root caps of Zea mays. J. Exp. Bot. 32, 779–788
Firn, R.D., Digby, J. (1977) The role of the peripheral cell layers in the geotropic curvature of sunflower hypocotyls: a new model of shoot geotropism. Aust. J. Plant Physiol. 4, 337–347
Firn, R.D., Digby, J. (1980) The establishment of tropic curvatures in plants. Annu. Rev. Plant Physiol. 31, 131–148
Fliry, M. (1932) Zur Wirkung der Endknospe auf die Hypokotylstreckung des Dikotylenkeimlings. Jahrb. Wiss. Bot. 77, 150–184
Gillespie, B., Briggs, W.R. (1961) Mediation of geotropic response by lateral transport of auxin. Plant Physiol. 36, 364–368
Gillespie, B., Thimann, K.V. (1963) Transport and distribution of auxin during tropistic response. I. The lateral migration of auxin in geotropism. Plant Physiol. 38, 214–225
Goldsmith, M.H.M., Wilkins, M.B. (1964) Movement of auxin in coleoptiles of Zea mays L. during geotropic stimulation. Plant Physiol. 39, 151–162
Greenwood, M.S., Shaw, S., Hillman, J.R., Ritchie, A., Wilkins, M.B. (1972) Identification of auxin from Zea coleoptile tips by mass spectrometry. Planta 108, 179–183
Hartung, W. (1976) Der basipetale [2-14C]Abscisinsäuretransport in Wurzeln intakter Bohnenkeimlinge und seine Bedeutung für den Wurzelgeotropismus. Planta 128, 59–62
Hild, V., Hertel, R. (1972) Initial phases of gravity-induced lateral auxin transport and geotropic curvature in corn coleoptiles. Planta 108, 245–258
Iwami, S., Masuda, Y. (1976) Distribution of labelled auxin in geotropically stimulated stems of cucumber and pea. Plant Cell Physiol. 17, 227–237
Juniper, B.E., Landau-Schachar, B., Audus, L.J. (1966) Root cap and the perception of gravity. Nature (London) 209, 93–94
Mentze, J., Raymond, B., Cohen, J.D., Rayle, D.R. (1977) Auxin-induced H+-secretion in Helianthus and its implications. Plant Physiol. 60, 509–512
Mulkey, T.J., Kuzmanoff, K.M., Evans, M.L. (1981) Correlations between proton-efflux patterns and growth patterns during geotropism and phototropism in maize and sunflower. Planta 152, 239–241
Phillips I.D.J. (1972) Endogenous gibberellin transport and biosynthesis in relation to geotropic induction of excised sunflower shoot-tips. Planta 96, 27–34
Pilet, P.E. (1979) Growth inhibitors in growing and geostimulated maize roots. In: Plant growth regulation, pp. 115–128, Pilet, P.E., ed. Springer, Berlin Heidelberg New York
Pilet, P.E., Rivier, L. (1981) Abscisic acid distribution in horizontal maize root segments. Planta 153, 453–458
Reisener, H.J. (1957) Versuche zum Geotropismus mit radioaktive β-Indolylessigsäure. Naturwissenschaften 44, 120
Schurzmann, M., Hild, V. (1980) Effect of indoleacetic acid, abscisic acid, root tips and coleoptile tips on growth and curvature of maize roots. Planta 150, 32–36
Shaw, S., Gardner, G., Wilkins, M.B. (1973) The lateral transport of IAA in intact coleoptiles of Avena sativa L. and Zea mays L. during geotropic stimulation. Planta 115, 97–111
Sievers, A., Hensel, W. (1982) The nature of graviperception. In: Plant growth substances 1982, pp. 479–506, Wareing, P.F., ed. Academic Press, London New York
Suzuki, T., Kondo, N. Fuji, T. (1979) Distribution of growth regulators in relation to the light-induced geotropic responsiveness in Zea roots. Planta 145, 323–329
Volkmann, D., Sievers, A. (1979) Graviperception in multicellular organs. In: Encyclopedia of plant physiology, N. S., vol. 7: Physiology of movements, pp. 567–600, Haupt, W., Feinleib, M.E., eds. Springer, Berlin Heidelberg New York
Weiler, E.W. (1980) Radioimmunoassays for the differential and direct analysis of free and conjugated abscisic acid in plant extracts. Planta 148, 262–272
Weiler, E.W. (1981) Radioimmunoassay for pmol-quantities of indole-3-acetic acid in plant extracts. Planta 153, 319–325
Weiler, E.W., Jourdan, P.J., Conrad, W. (1981) Levels of indole-3-acetic acid in intact and decapitated coleoptiles as determined by a specific and highly sensitive solid-phase enzyme immunoassay. Planta 153, 561–571
Went, F.W. (1928) Wuchsstoff und Wachstum. Rec. Trav. Bot. Neerl. 25, 1–116
Went, F.W., Thimann, K.V. (1937) Phytohormones. MacMillan. New York
Wilkins, M.B. (1977) Gravity and light-sensing guidance systems in primary roots and shoots. Symp. Soc. Exp. Biol. 31, 275–335
Wilkins, M.B. (1979) Growth-control mechanisms in gravitropism. In: Encyclopedia of plant physiology, N. S., vol. 7: Physiology of movements, pp. 601–626, Haupt, W., Feinleib, M.W., eds. Springer, Berlin Heidelberg New York
Wilkins, M.B., Gibbons, G.S.B., Shaw, S. (1970) The source and transport of growth regulators responsible for the geotropic response of Zea mays roots. In: Plant growth substances 1970, pp. 717–724, Carr, D.J., ed., Springer, Berlin Heidelberg New York
Wright, L.Z., Rayle, D.L. (1982) Inhibition of shoot geotropism by neutral buffers. Plant Physiol. 69, 278–279
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Mertens, R., Weiler, E.W. Kinetic studies on the redistribution of endogenous growth regulators in gravireacting plant organs. Planta 158, 339–348 (1983). https://doi.org/10.1007/BF00397336
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DOI: https://doi.org/10.1007/BF00397336