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Inhibition of auxin-stimulated growth of pea stem segments by a specific nonasaccharide of xyloglucan

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Abstract

Hemicellulose extracted from cell walls of suspension-cultured rose (Rosa “Paul's Scarlet”) cells was digested with cellulase from Trichoderma viride. The quantitatively major oligosaccharide products, a nonasaccharide and a heptasaccharide derived from xyloglucan, were purified by gel permeation chromatography. The nonasaccharide was found to inhibit the 2,4-dichlorophenoxy-acetic-acid-induced elongation of etiolated pea (Pisum sativum) stem segments. This confirms an earlier report (York et al., 1984, Plant Physiol. 75, 295–297). The inhibition of elongation by the nonasaccharide showed a maximum at around 10-9M with higher and lower concentrations being less effective. The heptasaccharide did not significantly inhibit elongation at 10-7–10-10M and also did not affect the inhibition caused by the nonasaccharide when co-incubated with the latter.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

XG:

xyloglucan

XG7:

xyloglucan heptasaccharide (Glc4·Xyl3)

XG9:

xyloglucan nonasaccharide (Glc4·Xyl3·Gal·Fuc)

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Authors and Affiliations

  1. Department of botany, University of Edinburgh, King's Buildings, Mayfield Road, EH9 3JH, Edinburgh, UK

    Gordon J. McDougall & Stephen c. Fry

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  1. Gordon J. McDougall
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  2. Stephen c. Fry
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McDougall, G.J., Fry, S.c. Inhibition of auxin-stimulated growth of pea stem segments by a specific nonasaccharide of xyloglucan. Planta 175, 412–416 (1988). https://doi.org/10.1007/BF00396348

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  • DOI: https://doi.org/10.1007/BF00396348

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