Abstract
The endosymbiotic Chlorella sp. from Paramecium bursaria excretes maltose both in the light and in the dark. Experiments on photosynthetic 14CO2 fixation and 14CO2 pulse-chase experiments show that maltose is synthesized in the light directly from compounds of the Calvin cycle, whereas in the dark it results from starch degradation.
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References
Bassham, J.A., Calvin, M. (1957) The path of carbon in photosynthesis. Englewood Cliffs, N.J., Prentice Hall, Inc.
Bassham, J.A., Kirk, M. (1960) Dynamics of the photosynthesis of carbon compounds. Biochim. Biophys. Acta 43, 447–464
Bassham, J.A., Kirk, M., Jensen, R.G. (1968) Photosynthesis by isolated chloroplasts. I. The diffusion of labelled photosynthetic intermediates between isolated chloroplasts and suspending medium. Biochim. Biophys. Acta 153, 211–218
Bligh, E.G., Dyer, W.J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917
Brown, J.A., Nielsen, P.J. (1974) Transfer of photosynthetically produced carbohydrate from endosymbiotic chlorellae to Paramecium bursaria. Protozool. 21, 569–570
Cernichiari, E., Muscatine, L., Smith, D.C. (1969) Maltose excretion by the symbiotic algae of Hydra viridis. Proc. R. Soc. (London) Ser. B. 173, 557–576
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F. (1956) Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356
Fitting, C., Doudoroff, M. (1952) Phosphorolysis of maltose by enzyme preparations from Neisseria meningitidis. J. Biol. Chem. 199, 153–163
Fogg, G.E. (1962) Extracellular products. In: Physiology and biochemistry of algae, p. 475–489 Lewin, R.A., ed. Academic Press New York London
Fogg, G.E. (1966) The extracellular products of algae. Oceanogr. Mar. Biol. Annu. Rev. 4, 195–212
Fogg, G.E., Westlake, D.F. (1955) The importance of extracellular products of algae in freshwater. Verh. Intern. Ver. Limnol. 12, 219–232
Heber, U., Hallier, U.W., Hudson, M. (1967) Untersuchungen zur intrazellulären Verteilung von Enzymen und Substraten in der Blattzelle II. Lokalisation von Enzymen des reduktiven und dem oxydativen Pentosephosphat-Zyklus in den Chloroplasten und Permeabilität der Chloroplasten-Membran gegenüber Metaboliten. Z. Naturforsch. 22b, 1200–1215
Heber, U., Walker, D.A. (1979) The chloroplast envelope—barrier or bridge? Trends Biochem. Sci. 4, 252–256
Heldt, H.W., Sauer, F., Rapley, L. (1971) Differentiation of the permeability properties of the two membranes of the chloroplast envelope. In: IInd International Congress on Photosynthesis. Stresa. 1345–1355
Karakashian, S.J. (1963) Growth of Paramecium bursaria as influenced by the presence of algal symbionts. Physiol. Zool. 36, 52–68
Levi, C., Gibbs, M. (1976) Starch degradation in isolated spinach chloroplasts. Plant Physiol. 57, 933–935
Linden, J.C., Tanner, W., Kandler, O. (1974) Properties of glucosyltransferase and glucan transferase from spinach. Plant Physiol. 54, 752–757
Lorenzen, H. (1959) Die photosynthetische Sauerstoffproduktion von Chlorella bei langfristig intermittierender Beleuchtung. Flora 147, 382–404
McKinney, G. (1941) Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–322
Muscatine, L., Karakashian, S.J., Karakashian, M.W. (1967) Soluble extracellular products of algae symbiotic with a ciliate, a sponge and a mutant Hydra. Comp. Biochem. Physiol. 20, 1–12
Reisser, W. (1975) Zur Taxonomie einer auxotrophen Chlorella aus Paramecium bursaria Ehrbg. Arch. Microbiol. 104, 293–295
Reisser, W. (1976) Die stoffwechselphysiologischen Beziehungen zwischen Paramecium bursaria Ehrbg. und Chlorella spec. in der Paramecium bursaria-Symbiose. I. Der Stickstoff- und der Kohlenstoff-Stoffwechsel. Arch. Microbiol. 107, 357–360
Robinson, S.P., Walker, D.A. (1979) The site of sucrose synthesis in isolated leaf protoplasts. FEBS Lett. 107, 295–299
Schilling, N., Kandler, O. (1975) α-Glucose-1-phosphate a precursor in the biosynthesis of maltose in higher plants. Biochem. Soc. Trans. 3, 985–987
Stange, L., Bennett, E.L., Calvin, M. (1960) Short-time 14CO2 incorporation experiments with synchronously growing Chlorella cells. Biochim. Biophys. Acta 37, 92–100
Takeda, H., Hirokawa, T. (1978) Studies on the cell wall of Chlorella. I. Quantitative changes in cell wall polysaccharides during the cell cycle of Chlorella ellipsoidea. Plant Cell Physiol. 19, 591–598
Taylor, D.L. (1973) Algal symbionts of invertebrates. Annu. Rev. Microbiol. 27, 171–187
Trench, R.K. (1979) The cell biology of plant-animal symbiosis. Annu. Rev. Plant Physiol. 30, 485–531
Urbach, W., Hudson, M.A., Ullrich, W., Santarius, K.A., Heber, U. (1965) Verteilung und Wanderung von Phosphoglycerat zwischen den Chloroplasten und dem Cytoplasma während der Photosynthese. Z. Naturforsch. 20b, 890–898
Watt, W.D., Fogg, G.E. (1966) The kinetics of extracellular glycolate production by Chlorella pyrenoidosa. J. Exp. Bot. 17, 117–134
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Ziesenisz, E., Reisser, W. & Wiessner, W. Evidence of de novo synthesis of maltose excreted by the endosymbiotic Chlorella from Paramecium bursaria . Planta 153, 481–485 (1981). https://doi.org/10.1007/BF00394991
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DOI: https://doi.org/10.1007/BF00394991