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Investigation of the biosynthesis of acetyl-CoA and oxaloacetic acid from pyruvic acid and the quantitative evaluation of incorporated 13C-labeled l-alanine in Arthrobacter hyalinus

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Abstract

Studies on the contribution to acetyl-CoA and oxaloacetic acid from the pyruvic acid transformation from l-alanine in Arthrobacter hyalinus were conducted by means of feeding experiments with l-[1-13C]alanine and l-[3-13C]alanine, followed by an analysis of the labeling patterns of coproporphyrinogen III using 13C NMR spectroscopy. The results demonstrated that l-alanine was transformed via pyruvic acid to both acetyl-CoA and oxaloacetic acid. Additionally, the quantitative analysis indicated that pyruvic acid was transformed to acetyl-CoA and oxaloacetic acid in the ratio of 1:0.8.

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Acknowledgment

The author would like to thank Prof. Kajiwara for helpful discussions.

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  1. Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose-shi, Tokyo, 204-8588, Japan

    Katsumi Iida

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  1. Katsumi Iida
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Correspondence to Katsumi Iida.

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Iida, K. Investigation of the biosynthesis of acetyl-CoA and oxaloacetic acid from pyruvic acid and the quantitative evaluation of incorporated 13C-labeled l-alanine in Arthrobacter hyalinus . J Radioanal Nucl Chem 301, 897–904 (2014). https://doi.org/10.1007/s10967-014-3172-4

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  • DOI: https://doi.org/10.1007/s10967-014-3172-4

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