Abstract
A sensitive enzyme-immunoassay was developed to quantify the tulip lectin and used to follow its distribution during the life cycle of tulips cv. Attila.
The tulip lectin is predominantly located in the bulbs. At planting time the absolute lectin concentration is approximately the same in all bulb scales. However, as the shoot grows and the plant turns on to flowering, the lectin concentration rapidly decreases, first in the inner bulb scales but later also in the outer bulb scale. Soon after flowering the lectin rapidly accumulates in the new daughter bulbs.
Lectin levels in leaves, stems and flowers are very low. The lectin in these tissues is already present before the sprout emerges. During the first two weeks after planting, there is a small increase in lectin concentration, followed by a rapid decrease as the plant turns on to flowering. By flowering time all the lectin has disappeared from the aerial parts.
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Abbreviations
- DW:
-
dry weight
- ELISA:
-
enzyme-linked immunosorbent assay
- FW:
-
fresh weight
- PBS:
-
phosphate-buffered saline
- PBSN:
-
phosphate-buffered saline containing 0.02% sodium azide
- PBST:
-
phosphate-buffered saline containing 0.02% sodium azide and 0.05% Tween 20
- TL:
-
tulip lectin
- Tris:
-
2-amino-2-(hydroxymethyl)-1,3-propanediol
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Van Damme, E.J.M., Peumans, W.J. Developmental changes and tissue distribution of lectin in Tulipa . Planta 178, 10–18 (1989). https://doi.org/10.1007/BF00392521
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DOI: https://doi.org/10.1007/BF00392521