Abstract
This work was done to determine whether the inorganic-pyrophosphate (PPi) content of plant tissues changes when the rate of glycolysis is altered. Treatment of excised clubs of the spadix of Arum maculatum L. and root apices of Pisum sativum L. with 2,4-dinitrophenol increased the rates of respiration but had no detectable effects on PPi contents. When the two tissues were subjected to up to 60 min anoxia, no changes in PPi were detected. Anoxia was shown to lead to a fall in ATP and concomitant rises in ADP and AMP in pea roots. It is argued (i) that variation in the rate of glycolysis was not accompanied by detectable changes in PPi content, (ii) that this observation does not favour the view that pyrophosphate fructose 6-phosphate 1-phosphotransferase mediates appreciable entry into glycolysis, and (iii) that PPi content can be maintained when respiratory-chain phosphorylation is inhibited.
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Abbreviations
- FW:
-
fresh weight
- PFK(PPi):
-
pyrophosphate fructose 6-phosphate 1-phosphotransferase
- PPi:
-
inorganic pyrophosphate
References
ap Rees, T. (1980) Assessment of the contributions of metabolic pathways to plant respiration. In: The biochemistry of plants, vol. 2: Metabolism and respiration, pp. 1–29, Davies, D.D., ed. Academic Press, New York
ap Rees, T. (1988) Hexose phosphate metabolism by nonphotosynthetic tissues of higher plants. In: The biochemistry of plants, vol. 14: Carbohydrates, pp. 1–33, Preiss, J., ed. Academic Press, New York
ap Rees, T., Beevers, H. (1960) Pathways of glucose dissimilation in carrot slices. Plant Physiol. 35, 830–838
ap Rees, T., Dancer, J.E. (1987) Fructose-2,6-bisphosphate and plant respiration. In: Plant mitochondria: structural, functional and physiological aspects, pp. 341–350, Moore, A.L., Beechey, R.B., eds. Plenum Press, New York
ap Rees, T., Fuller, W.A., Wright, B.W. (1976) Pathways of carbohydrate oxidation during thermogenesis by the spadix of Arum maculatum. Biochim. Biophys. Acta 347, 22–35
ap Rees, T., Green, J. H., Wilson, P.M. (1985) Pyrophosphate: fructose 6-phosphate 1-phosphotransferase and glycolysis in nonphotosynthetic tissues of higher plants. Biochem. J. 227, 299–304
ap Rees, T., Jenkin, L.E.T., Smith, A.M., Wilson, P.M. (1987) The metabolism of flood-tolerant plants In: Plant life in aquatic and amphibious habitats. pp. 227–238, Crawford, R.M.M., ed. Blackwell, Oxford
Beevers, H. (1953) 2,4-Dinitrophenol and plant respiration. Am. J. Bot. 40, 90–96
Black, C.C., Mustardy, L., Sung, S.S., Kormanik, P.P., Xu, D.-P., Paz, N. (1987) Regulation and roles for alternative pathways of hexose metabolism in plants. Physiol. Plant. 69, 387–394
Dancer, J.E., ap Rees, T. (1989) Phosphoribosyl pyrophosphate and the measurement of inorganic pyrophosphate in plant tissues. Planta 177, 261–264
Fowler, M.W., ap Rees, T. (1970) Carbohydrate oxidation during differentiation in roots of Pisum sativum. Biochim. Biophys. Acta 201, 33–44
Hargreaves, J.A., ap Rees, T. (1988) Sucrose and hexose metabolism by clubs of Typhanim giraldii and roots of Pisum sativum. Phytochemistry 27, 1621–1625
Hedrich, R., Rasche, K., Stitt, M. (1985) A role for fructose 2,6-bisphosphate in regulating carbohydrate metabolism in guard cells. Plant Physicl. 79, 977–982
Jaworek, D., Welsch, J. (1974) Adenosine 5′-diphosphate and adenosine 5′-monophosphate. In: Methods of enzymatic analysis, vol. 7, pp. 365–370, Bergmeyer, H.U., ed. Verlag-Chemie, Weinheim
Michal, G. (1974) D-glucose 6-phosphate and D-fructose 6-phosphate. In: Methods of enzymatic analysis, vol. VI, pp. 191–198, Bergmeyer, H.U., ed. Verlag Chemie, Weinheim
Michal, G. (1974b) D-fructose 1,6-bisphosphate, dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate. In: Methods of enzymatic analysis, vol VI, pp. 342–350. Bergmeyer, H.U., ed. Verlag Chemie, Weinheim
Smith, A.M., ap Rees, T. (1979) Effects of anaerobiosis on carbohydrate oxidation by roots of Pisum sativum. Phytochemistry 18, 1453–1458
Trautschold, I., Lamprecht, W., Schweitzer, G. (1974) UV-method with hexokinase and glucose-6-phosphate dehydrogenase. In: Methods of enzymatic analysis. vol. VII, pp. 346–357, Bergmeyer, H.-U., ed. Verlag-Chemie, Weinheim
Van Schaftingen, E., Hers, H.-G. (1983) Fructose 2,6-bisphosphate in relation with the resumption of metabolic activity in slices of Jerusalem artichoke tubers. FEBS Lett. 164, 195–200
Weiner, H., Stitt, M., Heldt, H.W. (1987) Subcellular compartmentation of pyrophosphate and alkaline pyrophosphatase in leaves. Biochim. Biophys. Acta 893, 13–21
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Dancer, J.E., Rees, T.a. Effects of 2,4-dinitrophenol and anoxia on the inorganic-pyrophosphate content of the spadix of Arum maculatum and the root apices of Pisum sativum . Planta 178, 421–424 (1989). https://doi.org/10.1007/BF00391871
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DOI: https://doi.org/10.1007/BF00391871