Summary
A cDNA sequence homologous to the Brassica self-incompatibility locus specific glycoprotein (SLSG) sequence was isolated from stigmas of B. oleracea plants homozygous for the S5 allele. The nucleotide sequence of this cDNA was obtained and compared with the S6 allelic form of the SLSG. Evidence is presented which indicates that this sequence does not specify the self-incompatibility response of pollen.
Similar content being viewed by others
Abbreviations
- SDS:
-
sodium dodecyl sulphate
- PVP:
-
polyvinylpyrrolidone
- BSA:
-
bovine serum albumin
- SLSG:
-
self-incompatibility locus specific glycoprotein
References
Anderson MA, Cornish EC, Mau S-L, Williams EG, Hoggart R, Atkinson A, Bonig I, Grego B, Simpson R, Roche PJ, Haley JD, Penscho JD, Niall HG, Tregear GW, Coghlan JP, Crawford RJ, Clarke AE (1986) Cloning of a cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata. Nature 321:38–44
Bennet MD, Smith JB (1976) Nuclear DNA amounts in Angiosperms. Philos Trans R Soc Lond 274:227–274
Croy RRD, Lycett GW, Gatehouse JA, Yarwood JN, Boulter D (1982) Cloning and analysis of cDNAs encoding plant storage protein precursors. Nature 295:76–79
De Nettancourt D (1977) Incompatibility in Angiosperms. Springer, Berlin
Evans IM, Croy RRD, Hutchinson P, Boulter D, Payne P, Gordon ME (1979) Cell free synthesis of some storage protein subunits by polyribosomes and RNA isolated from developing seeds of pea (Pisum sativum L.). Planta 144:455–462
Feinberg A, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Graham DE (1978) The isolation of high molecular weight DNA from whole organisms or large tissue masses. Anal Biochem 85:609–613
Heslop-Harrison J (1968) Ribosome site and S-gene action. Nature 218:90–91
Isogai A, Takayama S, Shiozawa H, Tsukamoto C, Kanbara T, Hinata K, Okazaki K, Suzuki A (1988) Existance of a common glycoprotein homologous to S-glycoproteins in two self-incompatible homozygotes of Brassica campestris. Plant Cell Physiol 29:1331–1336
Lalonde BA, Nasrallah ME, Dwyer KG, Chen C-H, Barlow B, Nasrallah JB (1989) A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene. Plant Cell 1:249–258
Logemann J, Schell J, Willmitzer L (1987) Improved method for the isolation of RNA from plant tissues. Anal Biochem 163:16–20
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbour Laboratory, New York
Nasrallah JB, Kao T-H, Goldberg ML, Nasrallah ME (1985) A cDNA clone encoding an S-locus specific glycoprotein from Brassica oleracea. Nature 318:263–267
Nasrallah JB, Kao T-H, Chen C-H, Goldberg ML, Nasrallah ME (1987) Amino-acid sequence of glycoproteins encoded by three alleles of the S-locus of Brassica oleracea. Nature 326:617–619
Nasrallah JB, Yu S-M, Nasrallah ME (1988) Self-incompatibility genes of Brassica oleracea: Expression, isolation and structure. Proc Natl Acad Sci USA 85:5551–5555
Sanger F, Coulson AR, Barrel BG, Smith AJH, Rose B (1980) Cloning in single stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143:161–178
Takayama S, Isogai A, Tsukamoto C, Ueda Y, Hinata K, Okasaki K, Susuki A (1987) Sequences of S-glycoproteins, products of the Brassica campestris self-incompatibility locus. Nature 326:102–105
Thomas PS (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77:5202–5205
Trick M, Flavell RB (1989) A homozygous S genotype of Brassica oleracea expresses two S-like genes. Mol Gen Genet 218:112–117
Whitehouse HLK (1950) Multiple-allelomorph incompatibility of pollen and style in the evolution of the angiosperms. Ann Bot 14:198–216
Woods D (1984) Oligonucleotide screening of cDNA libraries. Focus 6(3):1–3
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
Rights and permissions
About this article
Cite this article
Scutt, C.P., Gates, P.J., Gatehouse, J.A. et al. A cDNA encoding an S-locus specific glycoprotein from Brassica oleracea plants containing the S5 self-incompatibility allele. Mol Gen Genet 220, 409–413 (1990). https://doi.org/10.1007/BF00391746
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00391746