Abstract
Spinach (Spinacia oleracea L.) and barley (Hordeum vulgare L.) were grown in hydroponic culture with varying levels of orthophosphate (Pi). When leaves were fed with 20 mmol·l−1 Pi at low CO2 concentrations, a temporary increase of CO2 uptake was observed in Pi-deficient leaves but not in those from plants grown at 1 mmol·l−1 Pi. At high concentrations of CO2 (at 21% or 2% O2) the Pi-induced stimulation of CO2 uptake was pronounced in the Pi-deficient leaves. The contents of phosphorylated metabolites in the leaves decreased as a result of Pi deficiency but were restored by Pi feeding. These results demonstrate that there is an appreciable capacity for rapid Pi uptake by leaf mesophyll cells and show that the effects of long-term phosphate deficiency on photosynthesis may be reversed (at least temporarily) within minutes by feeding with Pi.
Similar content being viewed by others
Abbreviations
- Pi:
-
orthophosphate
References
Arnon, D.I. (1949) Copper enzyme in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15
Bielski, R.L. (1973) Phosphate pools, phosphate transport, and phosphate availability. Annu. Rev. Plant Physiol. 24, 225–252
Bottrill, D.E., Possingham, J.V., Kriedemann, P.E. (1970) The effect of nutrient deficiencies on photosynthesis and respiration in spinach. Plant Soil 32, 424–438
Bouma, D. (1975) Effects of metabolic phosphorus compounds on rates of photosynthesis of detached phosphorus-deficient subterranean clover leaves. J. Exp. Bot. 26, 52–59
Cockburn, W., Baldry, C.W., Walker, D.A. (1967a) Oxygen evolution by isolated chloroplasts with carbon dioxide as the hydrogen acceptor. A requirement for orthophosphate or pyrophosphate. Biochim. Biophys. Acta 131, 594–596
Cockburn, W., Baldry, C.W., Walker, D.A. (1967b) Some effects of inorganic phosphate on O2 evolution by isolated chloroplasts. Biochim. Biophys. Acta 143, 614–624
Dietz, K.-J., Heber, U. (1984) Rate limiting factors in leaf photosynthesis. I. Carbon fluxes in the calvin cycle. Biochim. Biophys. Acta 767, 432–443
Edwards, G.E., Walker, D.A. (1983) C3, C4 Mechanisms: Cellular and environmental regulation of photosynthesis, pp. 181–183, Blackwell Scientific publications, Oxford, London
Falkner, G., Horner, F., Simonis, W. (1980) The regulation of the energy dependent phosphate uptake by the blue green alga Anacystis nidulans. Planta 149, 138–143
Furbank, R.T., Walker, D.A. (1985) Photosynthetic induction in C4 leaves: An investigation using infra-red gas analysis and chlorophyll a fluorescence. Planta 163, 75–83
Harris, G.C., Cheesbrough, J.K., Walker, D.A. (1983) Measurement of CO2 and H2O vapour exchange in spinach leaf discs: Effect of orthophosphate. Plant Physiol. 71, 102–107
Heber, U., Willenbrink, J. (1964) Sites of synthesis and transport of photosynthetic products within the leaf cell. Biochim. Biophys. Acta 82, 313–324
Heldt, H.W., Chon, C.J., Maronde, D., Herold, A., Stankovic, Z.S., Walker, D.A., Kraminer, A., Kirk, M.R., Heber, U. (1977) The role of orthophosphate and other factors in the regulation of starch formation in leaves and isolated chloroplasts. Plant Physiol. 59, 1146–1155
Herold, A., Walker, D.A. (1979) Transport across chloroplast envelopes — the role of phosphate. In: Membrane transport in biology, vol. 2, pp. 411–439, Giebish, G., Tosterson, D., Ussing, H., eds. Springer, New York
Machler, F., Schnyder, H., Nosberger, J. (1984) Influence of inorganic phosphate on photosynthesis of wheat chloroplasts. I. Photosynthesis and assimilate export at 5° and 25°C. J. Exp. Bot. 35, 481–487
Mengel, K. (1969) Phosphorus. In: Handbuch für Pflanzenernährung, pp. 379–394, Linser, H., ed. Springer, New York
Michal, G., Beutler, H.-O. (1974) d-fructose-1,6-diphosphate, dihydroxyacetone phosphate and d-glyceraldehyde-3-phosphate. In: Methods of enzymatic analysis vol., pp. 1314–1319, Bergmeyer, H.U., ed. Academic Press, New York London
Natr, L. (1970) Effect of mineral nutrient deficiencies on dry matter production, photosynthetic intensity and N, P, and K quantities in plants. Physiol. Vég. 8, 573–583
Pirson, A., Tichy, C., Wilhelmi, G. (1952) Stoffwechsel und Mineralsalzernährung einzelliger Grimalgen. 1. Vergleichende Untersuchungen an Mangelkulturen von Ankistrodesmus. Planta 40, 199–253
Racker, E. (1974) D-ribulose-1,5-diphosphate. In: Methods of enzymatic analysis, vol. 3, pp. 1362–1364, Bergmeyer, H.U., ed. Academic Press, New York London
Rebeille, F., Bligny, R., Douce, R. (1982) Regulation of Pi uptake by Acer pseudoplatanus cells. Arch. Biochem. Biophys. 219, 371–378
Rebeille, F., Bligny, R., Martin, J.-B., Douce, R. (1983) Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch. Biochem. Biophys. 225, 143–148
Taussky, H.H., Shorr, E. (1953) A microcolorimetric method for the determination of inorganic phosphorus. J. Biol. Chem. 202, 675–685
Terry, N., Ulrich, A. (1973) Effects of phosphorus deficiency on the photosynthesis and respiration of leaves of sugar beet. Plant Physiol. 51, 43–47
Ullrich-Eberius, C.I., Novacky, A., van Bel, A.J.E. (1984) Phosphate uptake in Lemna gibba GI: energetics and kinetics. Planta 161, 46–52
Walker, D.A. (1980) Regulation of starch synthesis in leaves — the role of orthophosphate. In: Physiological aspects of crop productivity. Proc. 15th Colloq. Int. Potash Inst., Bern, pp. 195–207
Woodrow, I.E., Ellis, J.R., Jellings, A., Foyer, C.H. (1984) Compartmentation and fluxes of inorganic phosphate in photosynthetic cells. Planta 161, 525–530
Zalitis, J., Uram, M., Bowser, A.M., Feingold, D.S. (1972) UDP-glucose dehydrogenase from beef liver. Methods Enzymol. 28, 430–435
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dietz, KJ., Foyer, C. The relationship between phosphate status and photosynthesis in leaves. Planta 167, 376–381 (1986). https://doi.org/10.1007/BF00391342
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00391342