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The comparative ultrastructure and possible function of eyespots: Euglena granulata and Chlamydomonas eugametos

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Summary

The structure of Euglena granulata and Chlamydomonas eugametos has been studied using polarization and electron microscopy, cinematography, and chemical extraction procedures, with the main focus on the structure of the eyespot.

The 50–60 granules which form the extrachloroplastic eyespot of E. granulata are large bodies, up to 1200 mμ in diameter. They are found in the cytoplasm near the base of the reservoir and are associated with the parabasal body which contains a large crystal. The eyespot granules are contained within membranes having a unit membrane structure; 2 or 3 are usually present in a single eyespot packet; microtubules are also contained within the packet. The eyespot granules have the structure of a positive spherite and clearly exhibit birefringence; this structure is modified by fixation.

The granules of the chloroplastic eyespot of C. eugametos are about 75 mμ in diameter and are contained within the chloroplast in an ordered array. Occasionally, the eyespot contains elongate or helical bodies mixed with the granules. Extraction with organic solvents caused the removal of materials which formed the eyespot granules as well as that of the osmophilic globules in the chloroplasts.

Several hypotheses which concern the function of the eyespots in these and other species are discussed in the light of our results. the possible origin and demise of the eyespot granules are also discussed.

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References

  • Arnott, H.J., and R.M. Brown, Jr.: Ultrastructure of the eyespot and its possible significance in phototaxis of Tetracystis excentrica. J. Protozool., (in press) (1967).

  • —, and P.L. Walne: Ultrastructural investigations of Euglena granulata. (Abstr.). Amer. J. Bot. 53, 603 (1966).

    Google Scholar 

  • Batra, P., and G. Tollin: Phototaxis in Euglena. I. Isolation of the eye-spot granules and identification of the eye-spot pigments. Biochim. biophys. Acta (Amst.) 79, 371–378 (1964).

    Google Scholar 

  • Cobb, H.D.: An in vivo absorption spectrum of the eyespot of Euglena mesnili. Tex. J. Sci. 60, 231–235 (1963).

    Google Scholar 

  • DeRobertis, E.: Electron microscope observations on the submicroscopic organization of the retinal rods. J. biophys. biochem. Cytol. 2, 319–330 (1956).

    Google Scholar 

  • Engelmann, T.W.: Über Licht- und Farbenperception niederster Organismen. Arch. ges. Physiol. 29, 387–400 (1882).

    Google Scholar 

  • Fauré-Fremiet, E. et C. Rouiller: Le flagelle interne d'une Chrysomonadale: Chromulina psammobia. C.R. Acad. Sci. (Paris) 244, 2655–2657 (1957).

    Google Scholar 

  • Gojdics, M.: The genus Euglena. Madison: Univ. Wisc. Press 1953.

    Google Scholar 

  • Halldal, P.: Action spectra of phototaxis and related problems in Volvocales, Ulva gametes, and Dinophyceae. Physiol. Plant. (Kobenhaven) 11, 118–153 (1958).

    Google Scholar 

  • — Phototaxis in protozoa. In: Biochemistry and physiology of protozoa (S. Hutner, ed.), vol. 3, p. 277–296. New York: Academic Press 1964.

    Google Scholar 

  • Hartshorne, J.N.: The function of the eyespot in Chlamydomonas. New Phytologist 52, 292–297 (1953).

    Google Scholar 

  • Hess, W.M.: Fixation and staining of fungus hyphae and host plant root tissues for electron microscopy. Stain Techol. 41, 27–35 (1966).

    Google Scholar 

  • Jacks, T.J., L.Y. Tatsu, and A.M. Altschul: Isolation and characterization of peanut spherosomes. Plant Physiol. 42, 585–597 (1967).

    Google Scholar 

  • Lang, N.J.: Electron microscopy of the Volvocaceae and Astrephomenaceae. Amer. J. Bot. 50, 280–300 (1963).

    Google Scholar 

  • Lasansky, A., and E. De Robertis: Submicroscopic analysis of the genetic dystrophy of visual cells in C3H mice. J. biophys. biochem. Cytol. 7, 679–684 (1960).

    PubMed  Google Scholar 

  • Ledbetter, M.C., and K.R. Porter: Morphology of microtubules of plant cells. Science 144, 872–874 (1964).

    Google Scholar 

  • Leedale, G.F.: Euglenoid flagellates. Englewood Cliffs, N.J.: Prentice Hall 1967.

    Google Scholar 

  • —, B.J.D. Meeuse, and E.G. Pringsheim: Structure of Euglena spirogyra. I and II. Arch. Mikrobiol. 50, 68–102 (1965).

    Google Scholar 

  • Lembi, C.A., and N.J. Lang: Electron microscopy of Carteria and Chlamydomonas. Amer. J. Bot. 52, 464–477 (1965).

    Google Scholar 

  • Mann, I.: The development of the human eye. London: Cambridge University Press 1928.

    Google Scholar 

  • Manton, I., and B. Clarke: Observations with the electron microscope on the internal structure of the spermatoid of Fucus. J. expt. Bot. 7, 416–432 (1956).

    Google Scholar 

  • Mast, S.O.: Structure and function of the eyespot in unicellular and colonial organisms. Arch. Protistenk. 60, 197–330 (1927).

    Google Scholar 

  • Mignot, J.P.: Structure et ultrastructure de quesques Euglénomonadines. Protistologica 2, 51–117 (1966).

    Google Scholar 

  • Mollenhauer, H.H.: Plastic embedding mixtures for use in electron microscopy. Stain Technol. 39, 111–114 (1964).

    PubMed  Google Scholar 

  • Ringo, D.L.: Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas. J. Cell Biol. 33, 543–571 (1967).

    Article  PubMed  Google Scholar 

  • Rosso, S.W.: Ultrastructural observations on the chromoplasts of red tomatoes. J. Ultrastruct. Res., (in press).

  • Sager, R., and G.E. Palade: Structure and development of the chloroplast in Chlamydomonas. I. The normal green cell. J. biophys. biochem. Cytol. 3, 463–488 (1957).

    PubMed  Google Scholar 

  • Sitte, P.: Hexagonale Anordnung der Globuli in Moos-Chloroplasten. Protoplasma (Wien) 56, 197–201 (1963).

    Google Scholar 

  • Strasburger, E.: Wirkung des Lichtes und der Wärme auf Schwärmsporen. Jena. Z. bed. Naturw. 12, 551 (1878).

    Google Scholar 

  • Straus, W.: Studies on the chromoplasts of carrots. Protoplasma (Wien) 53, 405–421 (1961).

    Google Scholar 

  • Trabucchi, B.: Ricerche al microscopio eletronico sullo sviluppo e sulla struttura dei chromoplasti di carota. Ann. Fac. Agrar. U.C.S.C. 4, 135–147 (1964).

    Google Scholar 

  • Walne, P.L.: The effects of colchicine on cellular organization in Chlamydomonas. I. Light microscopy and cytochemistry. Amer. J. Bot. 53, 908–916 (1966).

    Google Scholar 

  • — The effects of colchicine on cellular organization in Chlamydomonas. II. Ultrastructure. Amer. J. Bot. 54, 564–577 (1967).

    Google Scholar 

  • —, and H.J. Arnott: Ultrastructure of stigmata in Chlamydomonas eugametos and Euglena granulata. (Abstr.) J. Physol 2, 5 (1966).

    Google Scholar 

  • Willmer, E.N.: The physiology of vision. Ann. Rev. Physiol. 17, 339–366 (1955).

    Article  Google Scholar 

  • Wolken, J.J.: Euglena. New Brunswick, N.J.: Rutgers University Press 1967.

    Google Scholar 

  • —, and E. Shin: Photomotion in Euglena gracilis. I. Photokinesis, II. Phototaxis. J. Protozool. 5, 39–46 (1958).

    Google Scholar 

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Authors and Affiliations

  1. The Department of Botany, The University of Tennessee, Knoxville

    Patricia L. Walne & Howard J. Arnott

  2. The Cell Research Institute, The University of Texas, Austin

    Patricia L. Walne & Howard J. Arnott

Authors
  1. Patricia L. Walne
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  2. Howard J. Arnott
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Additional information

Supported in part by NSF Grant GB-313. We thank Dr. Harold C. Bold and Dr. W. Gordon Whaley for their support and encouragement, Dr. R.M. Brown, jr. and Dr. Tom Kantz for aid in cinephotography, Dr. Peter Sitte for his help with polarization microscopy, and Mrs. Virginia Stork for her excellent technical assistance.

Contribution No. 286 from the Department of Botany, The University of Tennessee, Knoxville.

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Walne, P.L., Arnott, H.J. The comparative ultrastructure and possible function of eyespots: Euglena granulata and Chlamydomonas eugametos . Planta 77, 325–353 (1967). https://doi.org/10.1007/BF00389319

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