Summary
Phloem proteins of the sieve tube exudate from Cucurbita maxima Duch. and Cucurbita pepo L. were investigated as to their filament forming ability in vitro. From the two main proteins (116000 dalton, 30000 dalton) only the 116000 dalton protein was found to form reversibly distinct filaments of 6–7 nm diameter upon removal of SH-protecting agents from the buffer, whereas the 30000 dalton protein was precipitated as amorphous material under these conditions. The protein filaments were similar to the filaments ocurring within the sieve tube cells in vivo.
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Abbreviations
- SDS:
-
sodium dodecyl sulfate
- TCA:
-
trichloroacetic acid
References
Beyenbach, J., Weber, C., Kleinig, H.: Sieve-tube proteins from Cucurbita maxima. Planta (Berl.) 119, 113–124 (1974)
Cronshaw, J.: P-porteins. In: Pholoem transport (S. Aronoff, S.J. Dainty, L. Srivastava, and C.A. Swanson, eds.). New York: Plenum, 1975 (in press)
Cronshaw, J., Esau, K.: Tubular and fibrillar components of mature and differentiating sieve elements. J. Cell Biol. 34, 801–815 (1967)
Cronshaw, J., Gilder, J., Stone, D.: Fine structural studies of P-proteins in Cucurbita, Cucumis, and Nicotiana. J. Ultrastruct. Res. 45, 192–205 (1973)
Eschrich, W., Evert, R. F., Heyser, W.: Proteins of the sieve tube exudate of Cucurbita maxima. Planta (Berl.) 100, 208–221 (1971)
Hepler, P. K., Palevitz, B. A.: Microtubules and microfilaments. Ann. Rev. Plant Physiol. 25, 309–362 (1974)
Kleinig, H.: Biochemistry of phloem proteins. In: Phloem transport (S. Aronoff, S.J. Dainty, L. Srivastava, and C.A. Swanson, eds.). New York: Plenum 1975 (in press)
Kleinig, H., Dörr, I., Weber, C., Kollmann, R.: Filamentous proteins from plant sieve tubes. Nature (Lond.) New Biol. 229, 152–153 (1971a)
Kleinig, H., Dörr, I., Kollmann, R.: Vinblastine-induced precipitation of phloem proteins in vitro. Protoplasma (Wien) 73, 293–302 (1971b)
Kollmann, R., Dörr, I., Kleinig, H.: Portein filaments—structural components of the phloem exudate. I. Observations with Cucurbita and Nicotiana. Plata (Berl.) 95, 86–94 (1970)
Kollmann, R.: Sieve element structure in relation to function. In: Phloem transport (S. Aronoff, S.J. Dainty, L. Srivastava, and C.A. Swanson, eds.). New York: Plenum 1975 (in press)
Laemmli, N.: Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (Lond.) 227, 680–684 (1970)
Lowry, D. H., Rosebrough, N. J., Farr, N. L., Randall, R. L.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Mollenhauer, H. H.: Plastic embedding mictures for use in electron microscopy. Stain Technol. 39, 111–114 (1964)
Walker, T. S.: The purification and some properties of a protein causing gelling in phloem sieve tube exudate from Cucurbita pepo. Biochim. biophys. Acta (Amst.) 257, 433–444 (1972)
Walker, T. S., Thaine, R.: Proteins and fine structural components in exudate from sieve tubes in Cucurbita pepo stems. Ann. Bot. 35, 773–790 (1971)
Weber, C., Franke, W. W., Kartenbeck, J.: Structure and biochemistry of phloem-proteins isolated from Cucurbita maxima. Exp. Cell Res. 87, 79–106 (1974)
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Kleinig, H., Thönes, J., Dörr, I. et al. Filament formation in vitro of a sieve tube protein from Cucurbita maxima and Cucurbita pepo . Planta 127, 163–170 (1975). https://doi.org/10.1007/BF00388377
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DOI: https://doi.org/10.1007/BF00388377