Abstract
The gibberellins (GAs) of both vegetative (leaves and stems) and reproductive (pods and seeds) tissue of the G2 strain of peas Pisum sativum L. were characterized in purified extracts by a combination of sequential silicic-acid partition column chromatography, and gas chromatography-mass spectrometry. Gibberellins A19, A20, A29 and an A29 catabolite were identified in both types of tissue. Gibberellins A9, A17 and A44 were also found in pods and seeds.
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Abbreviations
- FID:
-
Ilame ionization detector
- GA(s):
-
gibberellin(s)
- GC:
-
gas chromatograph(y)
- HPLC:
-
high performance liquid chromatograph(y)
- LD:
-
long day
- MS:
-
mass spectrum(a) or mass spectrometer(ry)
- SD:
-
short day
References
Bearder, J.R. (1980) Plant hormones and other growth substances-their background structure and occurrence. In: Encyclopedia of plant physiology, N.S., vol. 9: Hormonal regulation of development. I. Molecular aspects of plant hormones, pp. 9–112, MacMillan, J., ed. Springer, Berlin Heidelberg New York
Binks, R., MacMillan, J., Pryce, R.J. (1969) Plant hormones. VIII. Combined gas chromatography-mass spectrometry of the methy esters of gibberellin A1 to A24 and their trimethyl silyl ethers. Phytochemistry 8, 271–284
Crozier, A., Kuo, C.C., Durley, R.C., Pharis, R.P. (1970) The biological activities of 26 gibberellins in nine plant bioassays. Can. J. Bot. 48, 867–877
Durley, R.C., Sassa, T., Pharis, R.P. (1979) Metabolism of tritiated gibberellin A20 in immature seeds of dwarf pea, ev.. Meteor. Plant Physiol. 64, 214–219
Frankland, B., Wareing, P.F. (1960) Effect of gibberellic acid on hypocotyl growth of lettuce seedlings. Nature (London) 185, 255–256
Frydman, V.M., MacMillan, J. (1973) Identification of gibberellins A20 and A29 in seed of Pisum sativum cv. Progress No. 9 by combined gas chromatography-mass spectrometry. Planta 115, 11–15
Frydman, V., Gaskin, P., MacMillan, J. (1974) Qualitative and quantitative analyses of gibberellins throughout seed maturation in Pisum sativum cv. Progress No. 9. Planta 118, 123–132
Gaskin, P., Kirkwood, P.S., MacMillan, J. (1981) Partial synthesis of ent-13-hydroxy-2-oxo-20-norgibberella-1(10), 16-diene-7, 19-dioic acid, a catabolite of gibberellin A29, and of related compounds. J. Chem. Soc. Perkin Trans. I, 1083–1091
Hedden, P., MacMillan, J., Phinney, B.O. (1978) The metabolism of the gibberellins. Annu. Rev. Plant. Physiol 29, 149–192
Heupel, R., Phinney, B.O., Hedden, P. (1981) Gibberellin metabolism in Zea mays L. (Abstr.). Plant Physiol. 67, Suppl., 102
Ingram, T.J., Browning, G. (1979) Influence of photoperiod on seed development in the genetic line of peas G2 and its relation to changes in endogenous gibberellins measured by combined gas chromatography-mass spectrometry. Planta 146, 423–432
Jones, M.G., Zeevaart, J.A.D. (1980a) Gibberellins and the photoperiodic control of stem elongation in the long day plant Agrostemma githago L. Planta 149, 269–273
Jones, M.G. and Zeevaart, J.A.D. (1980b) The effect of photoperiod on the level of seven endogenous gibberellins in the long day plant Agrostemma githago L. Planta 149, 274–279
Kamisaka, S. (1979) Catecholamine stimulation of the gibberellin action that induces lettuce hypocotyl elongation. Plant Cell Physiol. 20, 1199–1207
Kurogochi, S., Murofushi, N., Ota, Y., Takahashi, N. (1979) Identification of gibberellins in the rice plant and quantitative changes of gibberellin A19 throughout its life cycle. Planta 146, 185–191
Metzger, J.D., Zeevaart, J.A.D. (1980a) Identification of six endogenous gibberellins in spinach shoots. Plant Physiol. 65, 623–626
Metzger, J.D., Zeevaart, J.A.D. (1980b) Effect of photoperiod on the levels of endogenous gibberellin in spinach as measured by combined gas chromatography-selected ion current monitoring. Plant Physiol. 66, 844–846
Murakami, Y. (1968) A new bioassay, for gibberellins, “microdrop method”, and its use for testing extracts of rice and morning glory. Bot. Mag. (Tokyo) 81, 33–43
Proebsting, W.M., Davies, P.J., Marx, G.A. (1978) Photoperiodic control of apical senescence in a genetic line of peas. Plant Physiol. 58, 800–802
Proebsting, W.M., Davies, P.J., Marx, G.A. (1978) Photoperiodinduced changes in gibberellin metabolism in relation to apical growth and senescence in genetic lines of peas (Pisum sativum) L.). Planta 141, 231–238
Reeve, D.A., Crozier, A. (1975) Gibberellin bioassays. In: Gibberellins and plant growth, pp. 35–64, Krishmamoorthy, H.N., ed. Wiley Eastern, New Delhi
Ropers, H.J., Graebe, J.E., Gaskin, P., MacMillan, J. (1978) Gibberellin biosynthesis in a cell free system from immature seeds of Pisum sativum. Biochem. Biophys. Res. Commun. 80, 690–697
Sponsel, V.M. (1980a) Metabolism of gibberellins in immature seeds of Pisum sativum. In: Plant growth substances 1979, pp. 174–179, Skoog, F., ed. Springer, Berlin Heidelberg New York
Sponsel, V.M. (1980b) Gibberellin metabolism in legume seeds. In: Gibberellins chemistry, physiology and use. British Plant Growth Regulator Group Monogr. No. 5, pp. 49–62, Lenton, J.R., ed. BPGRG, Wantage, U.K.
Sponsel, V.M., MacMillan, J. (1977) Further studies on the metabolism of gibberellins (GAs) A9, A20 and A29 in immature seeds of Pisum sativum cv. Progress No. 9. Planta 135, 129–136
Sponsel, V.M., MacMillan, J. (1978) Metabolism of gibberellin A29 in seeds of Pisum sativum cv. Progress No. 9; use of [2H] and [3H] GAs, and the identification of a new GA catabolite. Planta 144, 69–78
Sponsel, V.M., MacMillan, J. (1980) Metabolism of [13C1] gibberellin A29 to [13C1] gibberellin catabolite in maturing seeds of Pisum sativum cv. Progress No. 9. Planta 150, 46–52
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Davies, P.J., Emshwiller, E., Gianfagna, T.J. et al. The endogenous gibberellins of vegetative and reproductive tissue of G2 peas. Planta 154, 266–272 (1982). https://doi.org/10.1007/BF00387873
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DOI: https://doi.org/10.1007/BF00387873