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Cell wall regeneration by protoplasts of Chlamydomonas

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Abstract

Protoplasts from Chlamydomonas smithii prepared by the action of C. reinhardii gamete autolysine have been studied with respect to cell wall regeneration. “Natural” protoplasts within sporangia were also investigated for purposes of comparison. In both cases a new cell wall is completed within 2–3 h of the onset of regeneration. The first visible stages of wall regeneration are to be seen after 40–60 min as a fine fringe outside of the plasmalemma. The development of the typical “central triplet” follows within the next 1 h. Cell wall regeneration is reversibly inhibited by cycloheximide (10μg ml-1) and reversibly disturbed by concanavalin A (50 μg ml-1). Actinomycin D at concentration over 100μg ml-1 also inhibit but the inhibition is irreversible and peculiar membrane effects are observed. Chelators (ethylenediamine tetraacetic acid; ethyleneglycol-bis-aminoethyl ether) and 2-deoxyglucose slightly retard or have no effect on cell wall regeneration.

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Abbreviations

EDTA:

ethylenediamine tetraacetic acid

EGTA:

ethyleneglycol-bis(aminoethyl ether)

N,N′:

tetraacetic acid

References

  • Burgess, J., Fleming, E.N.: Ultrastructural observations of cell wall regeneration around isolated tobacco protoplasts. J. Cell Sci. 14, 439–449 (1974)

    PubMed  Google Scholar 

  • Burgess, J., Linstead, P.J.: Ultrastructural studies of the binding of Concanavalin A to the plasmalemma of higher plant protoplasts. Planta 130, 73–79 (1976)

    Google Scholar 

  • Burgess, J., Linstead, P.J.: Coumarin inhibition of microfibril formation at the surface of cultured protoplasts. Planta 133, 267–273 (1977)

    Google Scholar 

  • Catt, J.W., Hills, G.J., Roberts, K.: A structural glycoprotein, containing hydroxyproline, isolated from the cell wall of Chlamydomonas reinhardii. Planta 131, 165–171 (1976)

    Google Scholar 

  • Claes, H.: Non-specific stimulation of the autolytic system in gametes from Chlamydomonas reinhardii. Exp. Cell Res. 108, 221–229 (1977)

    PubMed  Google Scholar 

  • Cocking, E.C.: Plant cell protoplasts-isolation and development. Ann. Rev. Plant Physiol. 23, 29–50 (1972)

    Google Scholar 

  • Davies, D.R.: Electrophoretic analyses of wall glycoproteins in normal and mutant cells. Exp. Cell Res. 73, 512–516 (1972)

    PubMed  Google Scholar 

  • Elorza, M.V., Sentandreu, R.: Effect of cycloheximide on yeast cell wall synthesis. Biochim. Biophys. Acta. 36, 741–747 (1969)

    Google Scholar 

  • Eriksson, T., Jonasson, K.: Nuclear division in isolated protoplasts from cells of higher plant grown in vitro. Planta 89, 85–89 (1969)

    Google Scholar 

  • Farkas, V., Svoboda, A., Bauer, S.: Inhibitory effect of 2-deoxy-D-glucose on the formation of the cell wall in yeast protoplasts. J. Bacteriol. 98, 744–748 (1969)

    PubMed  Google Scholar 

  • Farkas, V., Svoboda, A., Bauer, S.: Secretion of cell wall compounds by yeast protoplasts. Effects of 2-deoxy-D-glucose and cycloheximide. Biochem. J. 118, 755–758 (1970)

    PubMed  Google Scholar 

  • Hanke, D.E., Northoote, D.H.: Cell wall formation by soybean callus protoplasts. J. Cell Sci. 14, 29–50 (1974)

    PubMed  Google Scholar 

  • Hills, G.J.: Cell wall assembly in vitro from Chlamydomonas reinhardii. Planta 115, 17–23 (1973)

    Google Scholar 

  • Hills, G.J., Gurney-Smith, M., Roberts, K.: Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardii. II. Electron microscopy and optical diffraction analysis. J. Ultr. Res. 43, 179–192 (1973)

    Google Scholar 

  • Hills, G.J., Philips, J.M., Gay, M.R., Roberts, K.: Self-assembly of a plant cell wall in vitro. J. Mol. Biol. 96, 431–441 (1975)

    PubMed  Google Scholar 

  • Hyams, J., Davies, D.R.: The induction and characterization of cell wall mutants of Chlamydomonas reinhardii. Mutation Res. 14, 381–389 (1972)

    Google Scholar 

  • Kauss, H.: Plant Lectins. Progr. Bot. 38, 58–70 (1976)

    Google Scholar 

  • Kojak, L.P., Bretthauer, R.K.: Synthesis of exocellular phosphomannan by protoplasts of Hansenula holsti. Arch. Biochem. Biophys. 126, 764–770 (1968)

    PubMed  Google Scholar 

  • Lang, W., Chrispeels, M.J.: Biosynthesis and release of cell wall-like glycoproteins during the vegetative cell cycle of Chlamydomonas reinhardii. Planta 129, 183–189 (1976)

    Google Scholar 

  • Meyer, Y., Abel, W.O.: Importance of the wall for cell division and in the activity of the cytoplasm in cultured tobacco protoplasts. Planta 123, 33–40 (1975)

    Google Scholar 

  • Meyer, Y., Abel, W.O.: Budding and cleavage division of tobacco mexophyll protoplasts in relation to pseudowall and wall formation. Planta 125, 1–13 (1975)

    Google Scholar 

  • Minami, S.A., Goodenough, U.W.: Novel glycoprotein synthesis during differentiation in Chlamydomonas. J. Cell Biol 70, 264a (1976)

  • Nečas, O.: Cell wall synthesis in yeast protoplasts. Bact. Revs. 33, 149–170 (1971)

    Google Scholar 

  • Nečas, O., Svoboda, A., Kopecka, M.: The effect of cycloheximide (actidione) on cell wall synthesis in yeast protoplasts. Exp. Cell Res. 53, 291–293 (1968)

    PubMed  Google Scholar 

  • Nečas, O., Kopecka, M., Brichta, J.: Interpretation of surface structure in frozen-etched protoplasts of yeasts. Exp. Cell Res. 58, 411–419 (1969)

    PubMed  Google Scholar 

  • Nicolson, G.L.: The interaction of lectins with animal cell surfaces. Int. Rev. Cytol. 39, 89–190 (1974)

    PubMed  Google Scholar 

  • Pringsheim, E.G., Pringsheim, O.: Die Ernährung koloniebildender Volvocales. Biol. Zbl. 78, 937–971 (1959)

    Google Scholar 

  • Quader, H., Cherniak, J., Filner, P.: Participation of calcium in flagellar shortening and regeneration in Chlamydomonas reinhardtii. Exp. Cell Res. (in press)

  • Roberts, K.: Crystalline glycoprotein cell waslls of algae: their structure, composition and assembly. Phil. Trans. R. Soc. Lond. 268, 129–146 (1974)

    Google Scholar 

  • Roberts, K., Hills, G.J.: The crystalline glycoprotein cell wall of the green alga Chlorogonium elongatum: a structural analysis. J. Cell Sci. 21, 59–71 (1976)

    PubMed  Google Scholar 

  • Roberts, K., Gurney-Smith, M., Hills, G.J.: Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardii. I. Ultrastructure and preliminary chemical analysis. J. Ultr. Res. 40, 599–613 (1972)

    Google Scholar 

  • Robinson, D.G., Ray, P.M.: The reversible cyanide inhibition of Golgi secretion in pea cells. Cytobiologie 15, 65–77 (1977)

    Google Scholar 

  • Roland, J.-C., Prat, R.: Les protoplastes et quelques problémes concernant le rôle et l'elaboration des parois. Coll. int. C.N.R.S. 212, 243–271 (1973)

    Google Scholar 

  • Schilde-Rentschler, L.: Role of the cell wall in the ability of tobacco protoplasts to form callus. Planta 135, 177–181 (1977)

    Google Scholar 

  • Schlösser, U.G.: Entwicklungsstadien- und sippenspezifische Zellwand-Autolysine bei der Freisetzung von Fortpflanzungszellen in der Gattung Chlamydomonas. Ber. Deutsch. Bot. Ges. 89, 1–56 (1976)

    Google Scholar 

  • Schlösser, U.G., Sachs, H., Robinson, D.G.: Isolation of protoplasts by means of a “species-specific” Autolysine in Chlamydomonas. Protoplasma 88, 51–64 (1976)

    PubMed  Google Scholar 

  • Sentandreu, R., Northcote, D.H.: Yeast cell wall synthesis. Biochem. J. 115, 231–240 (1969)

    PubMed  Google Scholar 

  • Sietsma, J.H., Child, J.J., Nesbitt, L.R., Haskins, R.H.: Ultrastructural aspects of wall regeneration by Pythium protoplasts. Ant. van Leeuwenhoek 41, 17–32 (1975)

    Google Scholar 

  • van der Valk, P., Wessels, J.G.H.: Ultrastructure and localization of wall polymers during regeneration and reversion of protoplasts of Schizophyllum commune. Protoplasma 90, 65–87 (1976)

    Google Scholar 

  • Williamson, F.A., Fowke, L.C., Weber, G., Constabel, F., Gamborg, O.: Microfibril deposition on cultured protoplasts of Vicia chajastana. Protoplasma 91, 213–220 (1977)

    Google Scholar 

  • Willison, J.H.M.: Fine structural changes occuring during the culture of isolated tomato fruit protoplasts. Coll. int. C.N.R.S. 212, 215–241 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-3400, Göttingen, Federal Republic of Germany

    David G. Robinson & Uwe G. Schlösser

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  1. David G. Robinson
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  2. Uwe G. Schlösser
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Robinson, D.G., Schlösser, U.G. Cell wall regeneration by protoplasts of Chlamydomonas . Planta 141, 83–92 (1978). https://doi.org/10.1007/BF00387749

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  • DOI: https://doi.org/10.1007/BF00387749

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