Abstract
In order to examine the effects of artificial diets at the tissue level, plaice (Pleuronectes platessa L.) were fed either high (50%) or low (20%) protein diets for several weeks and the total quantities of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), protein and phospholipid in their livers were measured. Alanine amino-transferase, aspartate aminotransferase, glutamate dehydrogenase and glucose-6-phosphatase activities in the livers were also measured. Similar measurements were made on the livers of wild plaice. The total amount of DNA in the livers of both cultured and wild plaice, of standard weight, did not differ significantly, irrespective of dietary history. In fish of standard weight, total hepatic RNA, protein and phospholipid levels were highest in the cultured fish fed high protein diet and lowest in the wild fish. These differences were related to dietary protein supply and environmental temperature. The total organ levels of the liver enzymes in plaice of equal weight were not significantly different in the cultured fish fed high and low protein diets. Wild-fish livers contained a smaller total amount of alanine aminotransferase, aspartate aminotransferase and glucose-6-phosphatase activity than did those of cultured fish. Total liver glutamate dehydrogenase activity was not significantly different in any of the fish examined. It is suggested that kinetic and allosteric factors may be more important in controlling protein metabolism and amino acid cat abolism in fish than total enzyme level.
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Literature Cited
Allen, R.J.L.: The estimation of phosphorous. Biochem. J. 34, 858–865 (1940)
Allison, J.B., R.W. Wannemacher, W.L. Banks, W.H. Wunner and R.A. Gomez-Brenes: Dietary proteins correlated with ribonuclease, ribonucleic acid and tissue proteins. J. Nutr. 78, 333–337 (1962)
Bartlett, G.R.: Phosphorous assay in column chromatography. J. biol. Chem. 234, 406–408 (1958)
Brett, J.R. and D.A. Higgs: Effect of temperature on the rate of gastric digestion in fingerling sockeye salmon, Oncorhynchus nerka. J. Fish. Res. Bd Can. 27, 1767–1779 (1970)
Bulow, F.J.: RNA-DNA ratios as indicators of recent growth rates of a fish. J. Fish. Res. Bd Can. 27, 2343–2349 (1970)
Ceriotti, G.: A microchemical determination of desoxyribonucleic acid. J. biol. Chem. 198, 297–303 (1952)
—: Determination of nucleic acids in animal tissues. J. biol. Chem. 214, 59–70 (1955)
Cowey, C.B., J.W. Adron, A. Blair and A. Shanks: Studies on the nutrition of marine flatfish. Utilization of various dietary proteins by plaice (Pleuronectes platessa). Br. J. Nutr. 31, 297–306 (1974)
—, J.A. Pope, J.W. Adron and A. Blair: Studies on the nutrition of marine flatfish. The protein requirement of plaice (Pleuronectes platessa). Br. J. Nutr. 28, 447–456 (1972)
Folch, J., M. Lees and G.H. Sloane-Stanley: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226, 497–509 (1957)
Freedland, R.A. and B. Szepesi: Control of enzyme activity: nutritional factors. In: Enzyme synthesis and degradation in mammalian systems, pp 103–140. Ed. by M. Rechcigl. Basel: S. Karger 1971
Haines, T.A.: Evaluation of RNA-DNA ratio as a measure of long term growth in fish populations. J. Fish. Res. Bd Can. 30, 195–199 (1973)
Krebs, H.A.: Some aspects of the regulation of fuel supply in omnivorous animals. Adv. Enzyme Regul. 10, 387–419 (1972)
—, D.A.H. Bennett, P. de Gasquet, T. Gascoyne and T. Yoshida: Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat kidney cortex slices. Biochem. J. 86, 22–27 (1963)
Munro, H.N.: Evolution of protein metabolism in mammals. In: Mammalian protein metabolism, Vol. 3. pp 133–182. Ed. by H.N. Munro. New York: Academic Press 1969
— and C.M. Clarke: The nutritional regulation of ribonucleic acid metabolism in the liver cell. Proc. Nutr. Soc. 19, 55–60 (1960)
— and E.D. Downie: Relationship of liver composition to intensity of protein metabolism in different mammals. Nature, Lond. 203, 603–604 (1964)
— and A. Fleck: Analysis of tissues and body fluids for nitrogenous constituents. In: Mammalian protein metabolism, Vol. 3. pp 423–525. Ed. by H.N. Munro. New York: Academic Press 1969
Nordlie, R.C. and W.J. Arion: Glucose-6-phosphatase. In: Methods in enzymology, Vol. 9. pp 619–625. Ed. by S.P. Colowick and N.O. Kaplan. New York: Academic Press 1966
Schimke, R.T.: Adaptive characteristics of urea cycle enzymes in the rat. J. biol. Chem. 237, 459–468 (1962)
Schmidt, G. and S.J. Thannhauser: A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues. J. biol. Chem. 161, 83–89 (1945)
Venugopalan, V.K.: Effect of growth hormone injection on the level of nucleic acids in the liver of intact fish (Ophicephalus striatus) Gen. Comp. Endocr. 8, 332–336 (1967)
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Communicated by J.H.S. Blaxter, Oban
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Cowey, C.B., Brown, D.A., Adron, J.W. et al. Studies on the nutrition of marine flatfish. The effect of dietary protein content on certain cell components and enzymes in the liver of Pleuronectes platessa . Mar. Biol. 28, 207–213 (1974). https://doi.org/10.1007/BF00387299
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DOI: https://doi.org/10.1007/BF00387299