Summary
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1.
In green leaves of the Angiospermae investigated here illumination causes a reversible increase in activity of NADP+-dependent glycerinaldehyde-3-phosphatedehydrogenase compared with the dark values.
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2.
Illumination does not show this effect on the NAD+-dependent enzyme.
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3.
Changing from dark to light and vice versa the new level of activity (by the present conditions) is attained in about 20 minutes.
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4.
The light-induced increase of activity also takes place in CO2-free atmosphere as well as after application of actinomycin C.
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5.
Chloramphenicol blocks the effect of light.
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6.
It is concluded that illumination causes an increased synthesis of the NADP+-dependent GPD and it is supposed that this is caused by the increased supply of cofactors.
Zusammenfassung
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1.
Belichtung bewirkt in den grünen Blättern der untersuchten Angiospermen eine reversible Aktivitätssteigerung der NADP+-spezifischen Glycerinaldehyd-3-phosphat-Dehydrogenase gegenüber den Dunkelwerten.
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2.
Das NAD+-spezifische Enzym zeigt diesen Effekt nicht.
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3.
Beim Übergan vom Dunkeln zum Hellen und umgekehrt wird (bei den gewählten Bedingungen) das neue Aktivitätsniveau in etwa 20 min erreicht.
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4.
Die lichtinduzierte Aktivitätssteigerung erfolgt auch in CO2-freier Atmosphäre und bei Zufuhr von Actinomycin C.
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5.
Chloramphenicol hemmt den Lichteffekt.
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6.
Es wird geschlossen, daß bei Belichtung eine verstärkte Synthese der NADP+-spezifischen GPD erfolgt, und vermutet, daß dies durch die vermehrte Anlieferung von Cofaktoren verursacht wird.
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Ziegler, H., Ziegler, I. Der Einfluss der Belichtung auf Die NADP+-Abhängige Glycerinaldehyd-3-Phosphat-Dehydrogenase. Planta 65, 369–380 (1965). https://doi.org/10.1007/BF00385415
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DOI: https://doi.org/10.1007/BF00385415