Summary
The application of 2,3,5-triiodobenzoic acid (TIBA, 10 mg·g-1 in lanolin) to the stem of intact pea seedlings (Pisum sativum L.) inhibited the basipetal transport of 14C from indoleacetic acid-1-14C (IAA-1-14C) applied to the apical bud, but not the transport of 14C in the phloem following the application of IAA-1-14C or sucrose-14C to mature foliage leaves. It was concluded that fundamentally different mechanisms of auxin transport operate in these two pathways.
When TIBA was applied at the same time as, or 3.0 h after, the application of IAA-1-14C to the apical bud, 14C accumulated in the TIBA-treated and higher internodes; when TIBA was applied 24.0 h before the IAA-1-14C, transport in the stem above the TIBA-treated internode was considerably reduced. TIBA treatments did not consistently influence the total recovery of 14C, or the conversion of free IAA to indoleaspartic acid (IAAsp). These results are discussed in relation to the possible mechanism by which TIBA inhibits auxin transport,.
Attention is drawn to the need for more detailed studies of the role of the phloem in the transport of endogenous auxin in the intact plant.
Similar content being viewed by others
Abbreviations
- TIBA:
-
2,3,5-triiodobenzoic acid
- IAAsp:
-
indoleaspartic acid
References
Audus, L. J., Thresh, R.: The effects of synthetic growth-regulator treatments on the levels of free endogenous growth-substances in plants. Ann. Bot. 20, 439–459 (1953).
Bonnemain, J.-L.: Transport et distribution des traceurs après application de AIA-2-14C sur les feuilles de Vicia faba. C. R. Acad. Sci, (Paris) (D) 273, 1699–1702 (1971).
Eschrich, W.: Translokation radioaktiv markierter Indolyl-3-Essigsäure in Siebröhren von Vicia faba. Planta (Berl.) 78, 144–157 (1968).
Field, R. J., Peel, A. J.: The movement of growth regulators and herbicides into the sieve elements of willow. New Phytologist 70, 997–1003 (1971).
Goldsmith, M. H. M.: The transport of auxin. Ann. Rev. Plant Physiol. 19, 347–360 (1968).
Goldsmith, M. H. M.: Transport of plant growth regulators. In: Physiology of plant growth and development (M. B. Wilkins, ed.), p. 127–162. London: McGraw-Hill 1969.
Hertel, R., Flory, R.: Auxin movement in corn coleoptiles. Planta (Berl.) 82, 123–144 (1968).
Hertel, R., Thomson, K.-St., Russo, V. E. A.: In vitro auxin binding to particulate cell fractions from corn coleoptiles. Planta (Berl.) 107, 325–340 (1972).
Hoad, G. V., Hillman, S. K., Wareing, P. F.: Studies on the movement of indole auxins in willow (Salix viminalis L.) Planta (Berl.) 99, 73–88 (1971).
Hollis, C. A., Tepper, H. B.: Auxin transport within intact dormant and active white ash shoots. Plant Physiol. 48, 146–149 (1971).
Kuse, G.: Effect of 2,3,5-triiodobenzoic acid on the growth of lateral buds and on tropism of petiole. Mem. Coll. Sci. Univ. Kyoto, Ser. B 20, 207–215 (1953).
Kuse, G.: Bud inhibition and correlative growth of petiole in sweet potato stem. Mem. Coll. Sci. Univ. Kyoto, Ser. B 21, 107–114 (1954).
Lembi, C. A., Morré, D. J., Thomson, K.-St., Hertel, R.: N-1-Naphthylphthalamicacid-binding of a plasma membrane-rich fraction from maize coleoptiles. Planta (Berl.) 99, 37–45 (1971).
Lepp, L. W., Peel, A. J.: Patterns of translocation and metabolism of 14C-labelled IAA in the phloem of willow. Planta (Berl.) 96, 62–73 (1971).
Little, E. C. S., Blackman, G. E.: The movement of growth regulators in plants. III. Comparative studies of transport in Phaseolus vulgaris. New Phytologist 62, 173–197 (1963).
Maxwell, F. G., Painter, R. H.: Auxins in the honeydew of Toxoptera graminum, Therioaphis maculata and Macrosiphum pisi, and their relation to the degree of tolerance in host plants. Bull. entomol. Soc. Amer. 55, 229–233 (1962).
McCready, C. C.: Translocation of growth regulators. Ann. Rev. Plant Physiol. 17, 283–294 (1966).
Morris, D. A., Briant, R. E., Thomson, P. G.: The transport and metabolism of 14C-Iabelled indoleacetic acid in intact pea seedlings. Planta (Berl.) 89, 178–197 (1969).
Morris, D. A., Kadir, G. O.: Pathways of auxin transport in the intact pea seedling (Pisum sativum L.). Planta (Berl.) 107, 171–182 (1972).
Osborne, D. J., Mullins, M. G.: Auxin, ethylene and kinetin in a carrier-protein model system for the polar transport of auxins in petiole segments of Phaseolus vulgaris. New Phytologist 68, 977–991 (1969).
Panigrahi, B. M., Audus, L. J.: Apical dominance in Vicia faba. Ann. Bot. 30, 457–473 (1966).
Pilet, P. E.: In vitro and in vivo auxin and cytokinin translocation. In: Biochemistry and physiology of plant growth substances (F. Wightman and G. Setterfield, eds.), p. 993–1004. Ottawa: Runge Press Ltd. 1968.
Rohrbaugh, L. M., Rice, E. L.: Effects of application of sugar on the translocation of sodium 2,4-dichlorophenoxyacetate by bean leaves in the dark. Bot. Gaz. 111, 85–89 (1949).
Vardar, Y.: A study on the apical bud inhibition upon lateral branches. Rev. Fac. Sci. Univ. Istanbul, Ser. B 20, 245–256 (1955).
Veen, H., Tammes, P. M. L.: Studies on phloem exudation from Yucca flaccida Haw. X. Translocation of indole-3-acetic acid. Acta Bot. Neerl. 20, 356–366 (1971).
Weintraub, R. L., Brown, J. W.: Translocation of endogenous growth regulators in the bean seedling. Plant Physiol. 25, 140–149 (1950).
Whitehouse, R. L., Zalik, S.: Translocation of indole-3-acetic acid-1′-14C and tryptophan-1-14C in seedlings of Phaseolus coccineus L. and Zea mays L. Plant Physiol. 42, 1363–1372 (1967).
Winter, A.: The promotion of the immobilization of auxin in Avena coleoptiles by triiodobenzoic acid. Physiol. Plantarum (Cph.) 20, 330–336 (1967).
Winter, A.: 2,3,5-Triiodobenzoic acid and the transport of 3-indoleacetic acid. In: Biochemistry and physiology of plant growth substances (F. Wightman and G. Setterfield, eds.), p. 1063–1076. Ottawa: Runge Press Ltd. 1968.
Winter, A., Thimann, K. V.: Bound indoleacetic acid in Avena coleoptiles. Plant Physiol. 41, 335–342 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morris, D.A., Kadir, G.O. & Barry, A.J. Auxin transport in intact pea seedlings (Pisum sativum L.): The inhibition of transport by 2,3,5-triiodobenzoic acid. Planta 110, 173–182 (1973). https://doi.org/10.1007/BF00384840
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00384840