Summary
The submandibular organ (a sort of apocrine sweat glands) of the rabbit was observed with the electron microscope. The cell structure of glandular tubules varies depending upon the secretory activity; there are three functional stages. The secretory cells at the resting stage are characterized by low height, absence of secretory substance, and presence of small and slender mitochondria.
In the synthesizing stage, enlargement and peculiar deformation of mitochondria are observed. Secretory substance always occurs near the deformed mitochondria. The part of a mitochondrion closely abutting on the secretion mass is extremely thin, and contains longitudinally oriented cristae. Sometimes a direct continuity is observed between the thinned portion of the deformed mitochondria and the mass of secretory substance. It is presumed that the secretion is initially produced in the mitochondria and then discharged from them. The Golgi apparatus and the rough surfaced endoplasmic reticulum may be involved indirectly. Smooth surfaced vesicles, probably related to the transport of raw material, are extremely abundant in the cells of this stage.
The development of a generally homogeneous projection into the gland lumen is characteristic of the stage of secretion discharge. The mitochondria are again small and slender, and the secretion is liquefied. At the base of the full-grown projection, cytoplasm is condensed to form a demarcation zone from which the projection may become detached. This mechanism of release of secretory product is quite the same as the so-called apocrine secretory process long postulated by light microscopists.
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References
Arakawa, K.: Electron microscopy of the liver, a review. [Japanese.] Sôgô Igaku (Tokyo) 18, 4–24 (1961).
Bargmann, W., K. Fleischhauer u. A. Knoop: Über die Morphologie der Milchsekretion. II. Zugleich eine Kritik am Schema der Sekretionsmorphologie. Z. Zellforsch. 53, 545–568 (1961).
—, u. A. Knoop: Über die Morphologie der Milchsekretion. Licht- und elektronenmikroskopische Studien an der Milchdrüse der Ratte. Z. Zellforsch. 49, 344–388 (1959).
Braunsteiner, H., K. Fellinger and F. Pakesch: Electron microscopic observations on the thyroid. Endocrinology 53, 123–133 (1953).
Caulfield, J. B.: Effects of varying the vehicle for OsO4 in tissue fixation. J. biophys. biochem. Cytol. 3, 827–830 (1957).
Haguenau, F.: The ergastoplasm, its history, ultrastructure, and biochemistry. Int. Rev. Cytol. 7, 425–483 (1958).
Hirsch, G. C.: Form- und Stoffwechsel der Golgi-Körper. Protoplasma Monographien, Bd. 18. Berlin: Springer 1939.
Iijima, T.: Electron microscope studies on the eccrine sweat gland of human axillary skin. [Japanese.] Acta anat. Nippon. 34, 649–672 (1959).
Irie, M.: Electron microscopic observation on the various mammalian thyroid glands. [Japanese.] Arch. histol. jap. 19, 39–74 (1960).
Junqueira, L. C. U., and G. C. Hirsch: Cell secretion, a study of pancreas and salivary glands. Int. Rev. Cytol. 5, 323–364 (1956).
Kitamura, T.: Electron microscope studies on the carpal organ of the pig. [Japanese.] Arch. histol. jap. 14, 575–610 (1958).
Kurosumi, K.: Electron microscopic analysis of the secretion mechanism. Int. Rev. Cytol. 11, 1–124 (1961).
Kurosumi, K., T. Iijima and T. Kitamura: Electron microscopy of the human eccrine sweat gland with special reference to the folding of plasma membrane. IV. Internat. Kongr. Elektr.-mikr., Berlin 1958b, Bd. 2, S. 361–365. Berlin: Springer 1960.
—, T. Kitamura and T. Iijima: Electron microscope studies on the human axillary apocrine sweat glands. Arch. histol. jap. 16, 523–566 (1959).
—, and K. Kano: Electron microscopy of the human sebaceous gland. Arch. histol. jap. 20, 235–246 (1960).
—, S. Shibasaki, G. Uchida and Y. Tanaka: Electron microscope studies on the gastric mucosa of normal rats. Arch. histol. jap. 15, 587–624 (1958a).
Lever, J. D.: Electron microscopic observations on the adrenal cortex. Amer. J. Anat. 97, 409–420 (1955).
—: The fine structure of brown adipose tissue in the rat with observations on the cytological changes following starvation and adrenalectomy. Anat. Rec. 128, 361–372 (1957).
Minamitani, K.: Zytologische und histologische Untersuchungen der Schweißdrüsen in der menschlichen Achselhaut. Okajimas Folia anat. jap. 21, 61–94 (1941).
Nakanishi, T.: On the ultrastructure of the submaxillary gland of the guinea pig. [Japanese.] J. Chiba med. Soc. (Chiba) 35, 474–492 (1959).
Napolitano, L., and D. W. Fawcett: The fine structure of brown adipose tissue in the newborn mouse and rat. J. biophys. biochem. Cytol. 4, 685–692 (1958).
Nicolas, J., C. Regaud et M. Favré: Sur la fine structure des glandes sudoripares de l'homme, particulièrement en cas que concerne les mitochondries et les phénomènes de sécrétion. 17th Intern. Congr. Med., Sect. 13, Dermatol. Syphilis, 1914, pp. 105–109.
Palade, G. E., and G. Schidlowsky: Functional association of mitochondria and lipide inclusions. Anat. Rec. 130, 352–353 (1958).
Palay, S. L.: The morphology of secretion. In: Frontiers in Cytology (S. L. Palay ed.), pp. 305–342. New Haven: Yale Univ. Press 1958.
Robertis, E. De, and D. Sabatini: Mitochondrial changes in the adrenal cortex of normal hamsters. J. biophys. biochem. Cytol. 4, 667–670 (1958).
Rogers, G. E.: Electron microscope observations on the structure of sebaceous gland. Exp. Cell Res. 13, 517–520 (1957).
Schaffer, J.: Die Hautdrüsenorgane der Säugetiere. Berlin: Urban & Schwarzenberg 1940.
Scott, B. L., and D. C. Pease: Electron microscopy of the salivary and lacrimal glands of the rat. Amer. J. Anat. 104, 115–161 (1959).
Suzuki, Y.: An electron microscopic study on fat drop formation in the liver cell cytoplasm. J. Electronmicr. (Tokyo) 9, 24–36 (1960).
Takaki, F., H. Sekiguchi, Y. Onodera, A. Nakamura and Y. Hongo: Electron microscopic studies on liver of mouse fed with Penicillium islandicum Sopp. [Japanese.] Denshi Kembikyo (Tokyo) 8, 154–156 (1959).
Yamamoto, T.: On the relationship between mitochondria and fat droplets in the hepatic cells of the mouse after administration of hydrocortisone. Arch. histol. jap. 15, 625–632 (1958).
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Kurosumi, K., Yamagishi, M. & Sekine, M. Mitochondrial deformation and apocrine secretory mechanism in the rabbit submandibular organ as revealed by electron microscopy. Z.Zellforsch 55, 297–312 (1961). https://doi.org/10.1007/BF00384325
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DOI: https://doi.org/10.1007/BF00384325