Skip to main content
SpringerLink
Log in

Nucleotide sequence of the split tRNA LeuUAA gene from Vicia faba chloroplasts: evidence for structural homologies of the chloroplast tRNALeu intron with the intron from the autosplicable Tetrahymena ribosomal RNA precursor

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The gene encoding the tRNA LeuUAA from broad bean chloroplasts has been located on a 5.1 kbp long BamHI fragment by analysis of the DNA sequence of an XbaI subfragment. This gene is 536 bp long and is split in the anticodon region. The 451 bp long intron shows high sequence homology over about 100 bp from each end with the corresponding regions of the maize chloroplast tRNA LeuUAA intron. These conserved sequences are probably involved in the splicing reaction, for they can be folded into a secondary structure which is very similar to the postulated structure of the intron from the autosplicable ribosomal RNA precursor of Tetrahymena. Very little sequence conservation is found in the 5′-and 3′-flanking regions of the broad bean and maize chloroplast tRNA LeuUAA genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abelson J (1979) RNA processing and the intervening sequence problem. Annu Rev Biochem 48:1035–1069

    Article  Google Scholar 

  • Anziano PQ, Hanson DK, Mahler HR, Perlman PS (1982) Functional domains in introns: trans-acting and cis-acting regions of intron 4 of the cob gene. Cell 30:925–932

    PubMed  Google Scholar 

  • Cech TR, Zaug AJ, Grabowski PJ (1981) In vitro splicing of the ribosomal RNA precursor of Tetrahymena: Involvement of a guanosine nucleotide in the excision of the intervening sequence. Cell 27:487–496

    PubMed  Google Scholar 

  • Church G, Gilbert W (1980) Yeast mitochondrial intron products required in trans for RNA splicing. In: Joseph DR, Schultz J, Scott WA, Werner R (eds) Mobilization and reassembly of genetic information. Academic Press, New York, pp 379–395

    Google Scholar 

  • Clewell DB, Helinski DR (1969) Supercolled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proc Natl Acad Sci USA 62:1159–1166

    PubMed  Google Scholar 

  • Davies RW, Waring RB, Ray JA, Brown TA, Scazzocchio C (1982) Making ends meet: a model for RNA splicing in fungal mitochondria. Nature 300:719–724

    PubMed  Google Scholar 

  • De la Salle H, Jacq C, Slonimski PP (1982) Critical sequences within mitochondrial introns: pleiotropic mRNA maturase and cis-dominant signals of the box intron controlling reductase and oxydase. Cell 28:721–732

    PubMed  Google Scholar 

  • Deno H, Kato A, Shinozaki K, Sugiura M (1982) Nucleotide sequences of tobacco chloroplast genes for elongator tRNAMet and tRNAVal (UAC): the tRNAVal (UAC) gene contains a long intron. Nucl Acids Res 10:7511–7520

    PubMed  Google Scholar 

  • Dujon B (1981) Mitochondrial genetics and functions. In: Molecular biology of the yeast Saccharomyces: Life cycle and inheritance. Cold Spring Harbor Laboratory, New York, pp 505–635

    Google Scholar 

  • Gauss DH, Sprinzl M (1983) Compilation of tRNA sequences. Nucl Acids Res 11:r1-r53; Compilation of sequences of tRNA genes. ibid. r55–r103

    PubMed  Google Scholar 

  • Grunstein M, Hogness DS (1975) Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961–3966

    PubMed  Google Scholar 

  • Herrmann RG, Bohnert HJ, Kowallik KV, Schmitt JM (1975) Size, conformation and purity of chloroplast DNA of some higher plants. Biochim Biophys Acta 378:305–317

    PubMed  Google Scholar 

  • Hollingsworth MJ, Hallick RB (1982) Euglena gracilis chloroplast transfer RNA transcription units: III. Nucleotide sequence analysis of a tRNATyr-tRNAHis-tRNAMet-tRNATrp-tRNAGlu-TRNAGly gene cluster. J Biol Chem 257:12795–12799

    Google Scholar 

  • Hoopes BC, McClure WR (1981) Studies on the selectivity of DNA precipitation by spermine. Nucl Acids Res 9:5493–5504

    PubMed  Google Scholar 

  • Jacq C, Lazowska J, Slonimski PP (1980) Sur un nouveau mécanisme de la régulation de l'expression génétique. CR Acad Sci Paris 290 (série D): pp 89–92

    Google Scholar 

  • Jacquier A, Dujon B (1983) The intron of the mitochondrial 21S rRNA gene: distribution in different yeast species and sequence comparison between Kluyveromyces thermotolerans and Saccharomyces cerevisia. Mol Gen Genet 192:487–499

    PubMed  Google Scholar 

  • Kaine BP, Gupta R, Woese CR (1983) Putative introns in tRNA genes of prokaryotes. Proc Natl Acad Sci USA 80:3309–3312

    PubMed  Google Scholar 

  • Karabin GD, Hallick RB (1983) Euglena gracilis chloroplast transfer RNA transcription units: Nucleotide sequence analysis of a tRNAThr-tRNAGly-tRNAMet-tRNAGln gene cluster. J Biol Chem 258:5512–5518

    PubMed  Google Scholar 

  • Koch W, Edwards K, Kossel H(1981) Sequencing of the 16S–23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes. Cell 25:203–213

    PubMed  Google Scholar 

  • Koller B, Delius H (1980) Vicia faba chloroplast DNA has only one set of ribosomal RNA genes as shown by partial denaturation mapping and R-loop analysis. Mol Gen Genet 178:261–269

    Article  Google Scholar 

  • Labouesse M, Slonimski PP (1983) Construction of novel cytochrome b genes in yeast mitochondria by substraction or addition of introns. The EMBO Journal 2:269–276

    PubMed  Google Scholar 

  • Lazowska J, Jacq C, Slonimski PP (1980) Sequence of introns and flanking exons of wild-type and box 3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron. Cell 22:333–348

    PubMed  Google Scholar 

  • Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. In: Grossman L, Moldave K (eds) Methods in enzymology,vol 65. Academic Press, New York, pp 499–556

    Google Scholar 

  • Michel F, Jacquier A, Dujon B (1982) Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structures. Biochimie 64:867–881

    PubMed  Google Scholar 

  • Michel F, Dujon B (1983) Conservation of RNA secondary structures in two intron families including mitochondrial-,chloroplast-and nuclear-encoded members. The EMBO Journal 2:33–38

    PubMed  Google Scholar 

  • Montandon PE, Stutz E (1983) Nucleotide sequence of a Euglena gracilis chloroplast genome region coding for the elongation factor Tu; evidence for a spliced mRNA. Nucl Acids Res 11:5877–5892

    PubMed  Google Scholar 

  • Mount SM (1982) A catalogue of splice junction sequences. Nucl Acids Res 10:459–472

    PubMed  Google Scholar 

  • Netter P, Jacq C, Carignami G, Slonimski PP (1982) Critical sequences within the mitochondrial introns: cis-dominant mutations of the “cytochrome-b-like” intron of the oxydase gene. Cell 28:733–738

    Article  Google Scholar 

  • Orozco EM, Hallick RB (1982) Euglena gracilis chloroplast transfer RNA transcription units: II. Nucleotide sequence analysis of a tRNAVal-tRNAAsn-tRNAArg-tRNALeu gene cluster. J Biol Chem 257:3265–3275

    PubMed  Google Scholar 

  • Osorio-Almeida ML, Guillemaut P, Keith G, Canaday J, Weil JH (1980) Primary structure of three leucine transfer RNAs from bean chloroplast. Biochem Biophys Res Commun 92:102–108

    PubMed  Google Scholar 

  • Roberts RJ (1974) Staphylococcal transfer ribonucleic acids: II. Sequence analysis of isoaccepting glycine transfer ribonucleic acids IA and IB from Staphylococcus epidermidis Texas 26. J Biol Chem 249:4787–4796

    PubMed  Google Scholar 

  • Rochaix JD, Malnoe P (1978) Anatomy of the chloroplast ribosomal DNA of Chlamydomonas reinhardii. Cell 15:661–670

    Article  PubMed  Google Scholar 

  • Schwarz Z, Jolly SO, Steinmetz AA, Bogorad L (1981) Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNAHis. Proc Natl Acad Sci USA 78:3423–3427

    Google Scholar 

  • Steinmetz A, Gubbins EJ, Bogorad L (1982) The anticodon of the maize chloroplast gene for tRNALeu(UAA) is split by a large intron. Nucl Acids Res 10:3027–3037

    PubMed  Google Scholar 

  • Steinmetz AA, Krebbers ET, Schwarz Z, Gubbins EJ, Bogorad L (1983) Nucleotide sequences of five maize chlorplast transfer RNA genes and their flanking regions. J Biol Chem 258:5503–5511

    PubMed  Google Scholar 

  • Stiegler GL, Matthews HM, Bingham SE, Hallick RB (1982) The gene for the large subunit of ribulose-1,5-bisphosphate carboxylase in Euglena gracilis chloroplast DNA: location, polarity, clonign and evidence for an intervening sequence. Nucl Acids Res 10:3427–3444

    PubMed  Google Scholar 

  • Takaiwa F, Sugiura M (1982) Nucleotide sequence of the 16S–23S spacer region in an rRNA gene cluster from tobacco chloroplast DNA. Nucl Acids Res 10:2665–2676

    PubMed  Google Scholar 

  • Weiss-Brummer B, Holl J, Schweyen RJ, Rodel G, Kaudewitz F (1983) Processing of yeast mitochondrial RNA: Involvement of intramolecular hybrids in splicing of cob intron 4 RNA by mutation and reversion. Cell 33:195–202

    PubMed  Google Scholar 

  • Wienand U, Schwarz Z, Feix G (1979) Electrophoretic elution of nucleic acids from gels adapted for subsequent biological tests. Application for analysis of mRNAs from maize endosperm. FEBS Lett 98:319–323

    Article  PubMed  Google Scholar 

  • Wild MA, Gall JG (1979) An intervening sequence in the gene coding for 25S ribosomal RNA of Tetrahymena pigmentosa. Cell 16:565–573

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, Université Louis Pasteur, 15, rue René Descartes, F-67084, Strasbourg, France

    Géraldine Bonnard, Jacques Henry Weil & André Steinmetz

  2. Centre de Génétique Moléculaire, Laboratoire Propre du Centre National de la Recherche Scientifique, associé à l'Université Pierre et Marie Curie, F-91190, Gif-sur-Yvette, France

    François Michel

Authors
  1. Géraldine Bonnard
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. François Michel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jacques Henry Weil
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. André Steinmetz
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J. Schell

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bonnard, G., Michel, F., Weil, J.H. et al. Nucleotide sequence of the split tRNA LeuUAA gene from Vicia faba chloroplasts: evidence for structural homologies of the chloroplast tRNALeu intron with the intron from the autosplicable Tetrahymena ribosomal RNA precursor. Molec. Gen. Genet. 194, 330–336 (1984). https://doi.org/10.1007/BF00383536

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00383536

Keywords

Search

Navigation