Abstract
A compound that stimulated growth of soybean callus was isolated from spring sap of sycamore (Acer pseudoplatanus L.). Insufficient compound was isolated to permit it to be characterised. A compound with identical properties was isolated from commercial maple syrup, the concentrated spring sap of Acer saccharum L. The compound was identified as 3-(3-methoxy-4-hydroxyphenyl)-propan-1-ol (dihydroconiferyl alcohol, DCA). DCA was also active in the tobacco callus and radish leaf senescence assays, but was inactive in four other tests for cytokinin activity. DCA acted synergistically with kinetin to promote soybean callus growth. It is concluded that DCA has properties distinct from those of purine cytokinins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- DCA:
-
dihydroconiferyl acohol
- GC:
-
gas chromatography
- IAA:
-
indole-3-acetic acid
- iP:
-
isopentenyladenine
- [9R]iP:
-
isopentenyladenosine
- LC:
-
liquid chromatography
- MS:
-
mass spectrometry
- NAA:
-
1-napthylacetic acid
- TLC:
-
thinlayer chromatography
- TMSi:
-
trimethylsilyl
References
Biddington, N.L., Thomas, T.H. (1973) A modified Amaranthus betacyanin bioassay for the rapid determination of cytokinins in plant extracts. Planta 111, 183–186
Brown, R., Rickless, P. (1949) A new method for the study of cell division and cell extension with some preliminary observation on the effect of temperature and of nutrients. Proc. R. Soc (London), Ser. B 136, 110–125
Eight peak index of mass spectra. 2nd edn. Mass Spectrometry Data Centre, Aldermaston
Fletcher, R.A., McCullagh, D. (1971) Cytokinin-induced chlorophyll formation in cucumber cotyledons. Planta 101, 88–90
Green, J.F., Muir, R.M. (1978) The effect of potassium on cotyledon expansion induced by cytokinins. Physiol. Plant. 43, 213–218
Higuchi, R., Aritomi, M., Donnelly, D.M.X. (1977) Monolignol and dilignol glycosides from Pinus contorta leaves. Phytochemistry 16, 1007–1011
Kamisaka, S. (1973) Requirement of cotyledons for gibberellic acid-induced hypocotyl elongation in lettuce seedlings. Isolation of the cotyledon factor active in enhancing the effect of gibberellic acid. Plant Cell Physiol. 14, 747–755
Kefford, N.P., Zwar, J.A., Bruce, M.I. (1968) Antogonism of purine and urea cytokinin activities by derivatives of benzylurea. In: Biochemistry and physiology of plant growth substances, pp 61–69, Wightman, F., Setterfield, D. eds. Runge Press, Ottawa
Letham, D.S. (1971) Regulators of cell division in plant tissues XII. A cytokinin bioassay using excised radish cotyledons. Physiol. Plant. 25, 391–396
Milborrow, B.V., Purse, J.G., Wightman, F. (1975) On the auxin activity of phenylacetic acid. Ann. Bot. 39, 1143–1146
Miller, C.O. (1963) Kinetic and kinetin-like compounds. In: Modern methods of plant analysis, Vol. 6, pp 194–202, Paech, K., Tracey, M.V., eds. Springer, Berlin
Miller, C.O. (1968) Naturally-occurring cytokinins. In: Biochemistry and physiology of plant growth substances, pp 33–45, Wightman, F., Setterfield, G., eds. Runge Press, Ottawa
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497
Phillips, D.A., Cleland, C.F. (1972) Cytokinin activity from the phloem sap of Xanthium strumarium. Planta 102, 173–178
Purse, J.G., Horgan, R., Horgan, J.M., Wareing, P.F. (1976) Cytokinins of sycamore spring sap. Planta 132, 1–8
Riley, R.G., Kolattukudy, P.E. (1975) Evidence for covalently attached p-coumaric acid and ferulic acid in cutins and suberins. Plant Physiol. 56, 650–654
Sakurai, N., Shibata, K., Kamisaka, S. (1975) Stimulation of auxin-induced elongation of cucumber hypocotyl sections by dihydroconiferyl alcohol. Dihydroconiferyl alcohol inhibits indole-3-acetic acid degration in vivo and in vitro. Plant Cell Physiol. 16, 845–855
Shibata, K., Kubota, K., Kamisaka, S. (1974) Isolation and chemical identification of a lettuce cotyledon factor, a synergist of the gibberellin action in inducing lettuce hypocotyl elongation. Plant Cell Physiol. 15, 191–194
Skoog, F., Strong, F.M., Miller, C.O. (1965) Cytokinins. Science 148, 532–533
Underwood, J.C., Filipic, V.J. (1963) J. Assoc. Offic. Anal. Chem. 46, 334–337
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lee, T.S., Purse, J.G., Pryce, R.J. et al. Dihydroconiferyl alcohol — A cell division factor from Acer species. Planta 152, 571–577 (1981). https://doi.org/10.1007/BF00380830
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00380830