Effects of nitrogen and phosphorus on blue grama growth and mycorrhizal infection

Summary

The purpose of this study was to examine the growth response of Bouteloua gracilis, with and without the vescular-arbuscular mycorrhiza (VAM), Glomus fasciculatus, to varying levels of phosphorus and nitrogen (as NH ⁴₊ ) and to determine whether nitrogen and phosphorus levels influence VAM establishment. Bouteloua gracilis was grown in 225 g of soil in a factorial experiment combining four levels of ammonium nitrogen (4, 30, 60, and 126 μg/g), four levels of phosphorus (3, 7, 12, and 22 μg/g), and with VAM spores or no spores. Bouteloua gracilis showed enhanced growth with increased nutrients over the entire range of experimental amendments. Shoot nitrogen concentration for all plants ranged from an average of 0.73% at the low amendment level to 1.61% at the high level, whereas shoot total averages ranged from 2.43 mg at the low amendment to 16.4 mg at the high amendment. Mean shoot phosphorus concentrations ranged from 0.109% at the low amendment level to 0.150% at the high amendment, while totals averaged 5.29 mg at the low amendment and 11.8 mg at the high amendment level. Infected plants were consistently smaller than uninfected plants. This reduction was significant at high nitrogen-low phosphorus, where percent infection was highest (71%). At low nitrogen levels, moderate infection (17%) was established at all phosphorus levels. No infection occurred when both nitrogen and phosphorus levels were high. The lack of a positive nutrient or biomass response to VAM establishment is contrary to most published reports, but is similar to a lack of response shown with certain grasses and other plants. It is possible that the parasitic nature of the response to infection represents the early phase of infection.